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Synthesis and application of a nitroboronic acid-substituated silica for affinity chromatography
Li Fanglou; Zhao Xinjie; Xu Guowang
关键词Affinity Chromatography Boronic Acid Affinity Chromatography Pre-column Multidimensional High Performance Liquid Chromatography Direct Injection
刊名CHINESE JOURNAL OF ANALYTICAL CHEMISTRY
2006-10-01
34期:10页:1366-1370
收录类别SCI
文章类型Article
WOS标题词Science & Technology ; Physical Sciences
类目[WOS]Chemistry, Analytical
研究领域[WOS]Chemistry
英文摘要A boronic acid-substituted silica was synthesized by using a gamma-glycidoxypropyltrimethoxysilane as the spacer arm and using a 3-amino-4-nitrobenzeneboronic acid as the ligand. The 3-amino-4-nitrobenzeneboronic acid was modified from m-aminobenzeneboronic acid. Then, boronic acid-substituted silica was packed in a stainless-steel column (35 mm x 4.6 mm i. d.) under the pressure of 2.07 MPa as an affinity pre-column. A coupled-column liquid chromatographic method for the direct analysis of 11 urinary nucleosides is described. Efficient on-line clean-up and concentration of 11 nucleosides from urine samples were obtained by the affinity pre-column as the first column (extraction column) and an ODS column (250 mm x 4.6 nun i. d.) as the second column (analytical column). The mobile phases applied consisted of 0.25 mol/L ammonium acetate (pH 7.95) on extraction column, and of 25 mmol/L potassium dihydrogen phosphate (pH 4.5) on analytical column, respectively. Determination of urinary nucleosides was performed on analytical column by using a linear gradient elution comprising 25 mmol/L potassium dihydrogen phosphate (pH 4.5) and methanol-water (60:40, V/V) with UV detection at 260 nm. Calibration plots of 11 standard nucleosides show good RSD, recovery and excellent linearity (r > 0. 995).
语种英语
WOS记录号WOS:000243502600002
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被引频次:5[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://cas-ir.dicp.ac.cn/handle/321008/140211
专题中国科学院大连化学物理研究所
作者单位Chinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatog Res & Anal Ctr, Dalian 116023, Peoples R China
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GB/T 7714
Li Fanglou,Zhao Xinjie,Xu Guowang. Synthesis and application of a nitroboronic acid-substituated silica for affinity chromatography[J]. CHINESE JOURNAL OF ANALYTICAL CHEMISTRY,2006,34(10):1366-1370.
APA Li Fanglou,Zhao Xinjie,&Xu Guowang.(2006).Synthesis and application of a nitroboronic acid-substituated silica for affinity chromatography.CHINESE JOURNAL OF ANALYTICAL CHEMISTRY,34(10),1366-1370.
MLA Li Fanglou,et al."Synthesis and application of a nitroboronic acid-substituated silica for affinity chromatography".CHINESE JOURNAL OF ANALYTICAL CHEMISTRY 34.10(2006):1366-1370.
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