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题名: Studying protein-drug interaction by microfluidic chip affinity capillary electrophoresis with indirect laser-induced fluorescence detection
作者: Liu, Xiaojun;  Liu, Xin;  Liang, Aiye;  Shen, Zheng;  Zhang, Yu;  Dai, Zhongpeng;  Xiong, Bohui;  Lin, Bingcheng
关键词: binding constant ;  indirect LIF ;  microfluidic chip-affinity CE
刊名: ELECTROPHORESIS
发表日期: 2006-08-01
DOI: 10.1002/elps.200500890
卷: 27, 期:15, 页:3125-3128
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology ;  Life Sciences & Biomedicine ;  Physical Sciences
类目[WOS]: Biochemical Research Methods ;  Chemistry, Analytical
研究领域[WOS]: Biochemistry & Molecular Biology ;  Chemistry
英文摘要: We developed a microfluidic chip-affinity CE method based on indirect LIF detection to study protein-drug interactions. The interaction between heparin and BSA was quantitatively studied, as a model system. In our method, sodium fluorescein was chosen as background, and redistilled water as marker to monitor EOF The electrophoretic mobility changes of BSA were measured, with various concentrations of heparin added to the running buffer. Each run was completed within 80 s. The binding constant was determined to be (1.24 +/- 0.05) x 10(3) M-1, which was in good agreement with that reported in the literature.
关键词[WOS]: FIBROBLAST-GROWTH-FACTOR ;  FRONTAL ANALYSIS ;  HOMOGENEOUS IMMUNOASSAY ;  SERUM-ALBUMIN ;  BINDING ;  HEPARIN ;  MICROCHIP ;  PLASMA ;  TNT
语种: 英语
WOS记录号: WOS:000239912600015
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/140217
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Chinese Acad Sci, Dalian Inst Chem Phys, Dalian 116012, Peoples R China

Recommended Citation:
Liu, Xiaojun,Liu, Xin,Liang, Aiye,et al. Studying protein-drug interaction by microfluidic chip affinity capillary electrophoresis with indirect laser-induced fluorescence detection[J]. ELECTROPHORESIS,2006,27(15):3125-3128.
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