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Studying protein-drug interaction by microfluidic chip affinity capillary electrophoresis with indirect laser-induced fluorescence detection
Liu, Xiaojun; Liu, Xin; Liang, Aiye; Shen, Zheng; Zhang, Yu; Dai, Zhongpeng; Xiong, Bohui; Lin, Bingcheng
关键词Binding Constant Indirect Lif Microfluidic Chip-affinity Ce
刊名ELECTROPHORESIS
2006-08-01
DOI10.1002/elps.200500890
27期:15页:3125-3128
收录类别SCI
文章类型Article
WOS标题词Science & Technology ; Life Sciences & Biomedicine ; Physical Sciences
类目[WOS]Biochemical Research Methods ; Chemistry, Analytical
研究领域[WOS]Biochemistry & Molecular Biology ; Chemistry
关键词[WOS]FIBROBLAST-GROWTH-FACTOR ; FRONTAL ANALYSIS ; HOMOGENEOUS IMMUNOASSAY ; SERUM-ALBUMIN ; BINDING ; HEPARIN ; MICROCHIP ; PLASMA ; TNT
英文摘要We developed a microfluidic chip-affinity CE method based on indirect LIF detection to study protein-drug interactions. The interaction between heparin and BSA was quantitatively studied, as a model system. In our method, sodium fluorescein was chosen as background, and redistilled water as marker to monitor EOF The electrophoretic mobility changes of BSA were measured, with various concentrations of heparin added to the running buffer. Each run was completed within 80 s. The binding constant was determined to be (1.24 +/- 0.05) x 10(3) M-1, which was in good agreement with that reported in the literature.
语种英语
WOS记录号WOS:000239912600015
引用统计
文献类型期刊论文
条目标识符http://cas-ir.dicp.ac.cn/handle/321008/140217
专题中国科学院大连化学物理研究所
作者单位Chinese Acad Sci, Dalian Inst Chem Phys, Dalian 116012, Peoples R China
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GB/T 7714
Liu, Xiaojun,Liu, Xin,Liang, Aiye,et al. Studying protein-drug interaction by microfluidic chip affinity capillary electrophoresis with indirect laser-induced fluorescence detection[J]. ELECTROPHORESIS,2006,27(15):3125-3128.
APA Liu, Xiaojun.,Liu, Xin.,Liang, Aiye.,Shen, Zheng.,Zhang, Yu.,...&Lin, Bingcheng.(2006).Studying protein-drug interaction by microfluidic chip affinity capillary electrophoresis with indirect laser-induced fluorescence detection.ELECTROPHORESIS,27(15),3125-3128.
MLA Liu, Xiaojun,et al."Studying protein-drug interaction by microfluidic chip affinity capillary electrophoresis with indirect laser-induced fluorescence detection".ELECTROPHORESIS 27.15(2006):3125-3128.
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