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Determination of organic acids in 1,3-propanediol fermentation broth by reversed phase high performance liquid chromatography
Mou Ying; Wang Yuanhao; Xiu Zhilong; Wang Longxing; Xiao Hongbin
关键词Reversed Phase High Performance Liquid Chromatography Organic Acids Fermentation Broth 1 3-propanediol
刊名CHINESE JOURNAL OF ANALYTICAL CHEMISTRY
2006-09-01
34页:S183-S186
收录类别SCI
文章类型Article
WOS标题词Science & Technology ; Physical Sciences
类目[WOS]Chemistry, Analytical
研究领域[WOS]Chemistry
英文摘要A reversed phase high performance liquid chromatographic method on a column of Kromasil C-18 (5 mu m, 250 mm x 4. 6 mm i. d.) with an eluant containing 0.2% (V/V) phosphoric acid and acetonitrile (96. 5:3. 5, V/V) was used for the analysis of organic acids in 1,3-propanediol fermentation broth. The solution of samples was pretreated with chloroform. The formic acid, lactic acid, acetic acid, citric acid, succinic acid and fumaric acid were completely separated and determined in 8 minutes. The flow rate of mobile phase was 1.0 mL/min and the column temperature was 20 degrees C. The detection wavelength was 214 rim. The regression equation, the linear range, the correlation coefficient and detection limit of the standard curves were determined. The relative standard deviation (RSD, n = 5) was in the range of 1.0% - 2.4%, and the average recoveries of the method ranged from 97.7% to 100.5%. The method was proved to be a rapid, effective way to determine the organic acids in 1, 3-propanediol fermentation broth.
语种英语
WOS记录号WOS:000244539600045
引用统计
被引频次:4[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://cas-ir.dicp.ac.cn/handle/321008/140225
专题中国科学院大连化学物理研究所
作者单位1.Dalian Univ Technol, Dept Biosci & Biotechnol, Sch Environm & Biol Sci & Technol, Dalian 116024, Peoples R China
2.Chinese Acad Sci, Dalian Inst Chem Phys, Dalian 116023, Peoples R China
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GB/T 7714
Mou Ying,Wang Yuanhao,Xiu Zhilong,et al. Determination of organic acids in 1,3-propanediol fermentation broth by reversed phase high performance liquid chromatography[J]. CHINESE JOURNAL OF ANALYTICAL CHEMISTRY,2006,34:S183-S186.
APA Mou Ying,Wang Yuanhao,Xiu Zhilong,Wang Longxing,&Xiao Hongbin.(2006).Determination of organic acids in 1,3-propanediol fermentation broth by reversed phase high performance liquid chromatography.CHINESE JOURNAL OF ANALYTICAL CHEMISTRY,34,S183-S186.
MLA Mou Ying,et al."Determination of organic acids in 1,3-propanediol fermentation broth by reversed phase high performance liquid chromatography".CHINESE JOURNAL OF ANALYTICAL CHEMISTRY 34(2006):S183-S186.
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