DICP OpenIR
Determination of organic acids in 1,3-propanediol fermentation broth by reversed phase high performance liquid chromatography
Mou Ying; Wang Yuanhao; Xiu Zhilong; Wang Longxing; Xiao Hongbin
KeywordReversed Phase High Performance Liquid Chromatography Organic Acids Fermentation Broth 1 3-propanediol
Source PublicationCHINESE JOURNAL OF ANALYTICAL CHEMISTRY
2006-09-01
Volume34Pages:S183-S186
Indexed BySCI
SubtypeArticle
WOS HeadingsScience & Technology ; Physical Sciences
WOS SubjectChemistry, Analytical
WOS Research AreaChemistry
AbstractA reversed phase high performance liquid chromatographic method on a column of Kromasil C-18 (5 mu m, 250 mm x 4. 6 mm i. d.) with an eluant containing 0.2% (V/V) phosphoric acid and acetonitrile (96. 5:3. 5, V/V) was used for the analysis of organic acids in 1,3-propanediol fermentation broth. The solution of samples was pretreated with chloroform. The formic acid, lactic acid, acetic acid, citric acid, succinic acid and fumaric acid were completely separated and determined in 8 minutes. The flow rate of mobile phase was 1.0 mL/min and the column temperature was 20 degrees C. The detection wavelength was 214 rim. The regression equation, the linear range, the correlation coefficient and detection limit of the standard curves were determined. The relative standard deviation (RSD, n = 5) was in the range of 1.0% - 2.4%, and the average recoveries of the method ranged from 97.7% to 100.5%. The method was proved to be a rapid, effective way to determine the organic acids in 1, 3-propanediol fermentation broth.
Language英语
WOS IDWOS:000244539600045
Citation statistics
Cited Times:4[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://cas-ir.dicp.ac.cn/handle/321008/140225
Collection中国科学院大连化学物理研究所
Affiliation1.Dalian Univ Technol, Dept Biosci & Biotechnol, Sch Environm & Biol Sci & Technol, Dalian 116024, Peoples R China
2.Chinese Acad Sci, Dalian Inst Chem Phys, Dalian 116023, Peoples R China
Recommended Citation
GB/T 7714
Mou Ying,Wang Yuanhao,Xiu Zhilong,et al. Determination of organic acids in 1,3-propanediol fermentation broth by reversed phase high performance liquid chromatography[J]. CHINESE JOURNAL OF ANALYTICAL CHEMISTRY,2006,34:S183-S186.
APA Mou Ying,Wang Yuanhao,Xiu Zhilong,Wang Longxing,&Xiao Hongbin.(2006).Determination of organic acids in 1,3-propanediol fermentation broth by reversed phase high performance liquid chromatography.CHINESE JOURNAL OF ANALYTICAL CHEMISTRY,34,S183-S186.
MLA Mou Ying,et al."Determination of organic acids in 1,3-propanediol fermentation broth by reversed phase high performance liquid chromatography".CHINESE JOURNAL OF ANALYTICAL CHEMISTRY 34(2006):S183-S186.
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