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题名: Determination of organic acids in 1,3-propanediol fermentation broth by reversed phase high performance liquid chromatography
作者: Mou Ying;  Wang Yuanhao;  Xiu Zhilong;  Wang Longxing;  Xiao Hongbin
关键词: reversed phase high performance liquid chromatography ;  organic acids ;  1,3-propanediol ;  fermentation broth
刊名: CHINESE JOURNAL OF ANALYTICAL CHEMISTRY
发表日期: 2006-09-01
卷: 34, 页:S183-S186
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology ;  Physical Sciences
类目[WOS]: Chemistry, Analytical
研究领域[WOS]: Chemistry
英文摘要: A reversed phase high performance liquid chromatographic method on a column of Kromasil C-18 (5 mu m, 250 mm x 4. 6 mm i. d.) with an eluant containing 0.2% (V/V) phosphoric acid and acetonitrile (96. 5:3. 5, V/V) was used for the analysis of organic acids in 1,3-propanediol fermentation broth. The solution of samples was pretreated with chloroform. The formic acid, lactic acid, acetic acid, citric acid, succinic acid and fumaric acid were completely separated and determined in 8 minutes. The flow rate of mobile phase was 1.0 mL/min and the column temperature was 20 degrees C. The detection wavelength was 214 rim. The regression equation, the linear range, the correlation coefficient and detection limit of the standard curves were determined. The relative standard deviation (RSD, n = 5) was in the range of 1.0% - 2.4%, and the average recoveries of the method ranged from 97.7% to 100.5%. The method was proved to be a rapid, effective way to determine the organic acids in 1, 3-propanediol fermentation broth.
语种: 英语
WOS记录号: WOS:000244539600045
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/140225
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Dalian Univ Technol, Dept Biosci & Biotechnol, Sch Environm & Biol Sci & Technol, Dalian 116024, Peoples R China
2.Chinese Acad Sci, Dalian Inst Chem Phys, Dalian 116023, Peoples R China

Recommended Citation:
Mou Ying,Wang Yuanhao,Xiu Zhilong,et al. Determination of organic acids in 1,3-propanediol fermentation broth by reversed phase high performance liquid chromatography[J]. CHINESE JOURNAL OF ANALYTICAL CHEMISTRY,2006,34:S183-S186.
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