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Preparation of affinity matrices for endotoxin removal
Tang, SW; Kong, L; Ou, JJ; Zou, HF
关键词Sepharose Endotoxin Polymyxin b Affinity Matrix Removal Efficiency
刊名CHINESE JOURNAL OF ANALYTICAL CHEMISTRY
2006-04-01
34期:4页:455-458
收录类别SCI
文章类型Article
WOS标题词Science & Technology ; Physical Sciences
类目[WOS]Chemistry, Analytical
研究领域[WOS]Chemistry
关键词[WOS]PYROGEN ADSORPTION ; LIPOPOLYSACCHARIDE ; ADSORBENTS
英文摘要Three affinity matrices for adsorption of endooxin from aqueous solution were studied by covalently bonding polymyxin B varying hydrocarbon lengths to Sepharose CL-4B. The content of ligand and adsorption efficiency increased with increasing length of spacers. The effect of salt, pH, temperature and flow rates on endotoxin adsorption for affinity matrix 3 was investigated. It showed that in the range of NaCl concentration of 0.05-0.5 mol/L, pH 6-10, temperature 25-55 degrees C and flow rate of 0.20-0.80 mL/min, the adsorption efficiency was over 90%.
语种英语
WOS记录号WOS:000237325400005
引用统计
被引频次:5[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://cas-ir.dicp.ac.cn/handle/321008/140247
专题中国科学院大连化学物理研究所
作者单位Chinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatog Res & Anal Ctr, Dalian 116023, Peoples R China
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GB/T 7714
Tang, SW,Kong, L,Ou, JJ,et al. Preparation of affinity matrices for endotoxin removal[J]. CHINESE JOURNAL OF ANALYTICAL CHEMISTRY,2006,34(4):455-458.
APA Tang, SW,Kong, L,Ou, JJ,&Zou, HF.(2006).Preparation of affinity matrices for endotoxin removal.CHINESE JOURNAL OF ANALYTICAL CHEMISTRY,34(4),455-458.
MLA Tang, SW,et al."Preparation of affinity matrices for endotoxin removal".CHINESE JOURNAL OF ANALYTICAL CHEMISTRY 34.4(2006):455-458.
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