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题名: Preparation of affinity matrices for endotoxin removal
作者: Tang, SW;  Kong, L;  Ou, JJ;  Zou, HF
关键词: Sepharose ;  endotoxin ;  polymyxin B ;  affinity matrix ;  removal efficiency
刊名: CHINESE JOURNAL OF ANALYTICAL CHEMISTRY
发表日期: 2006-04-01
卷: 34, 期:4, 页:455-458
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology ;  Physical Sciences
类目[WOS]: Chemistry, Analytical
研究领域[WOS]: Chemistry
英文摘要: Three affinity matrices for adsorption of endooxin from aqueous solution were studied by covalently bonding polymyxin B varying hydrocarbon lengths to Sepharose CL-4B. The content of ligand and adsorption efficiency increased with increasing length of spacers. The effect of salt, pH, temperature and flow rates on endotoxin adsorption for affinity matrix 3 was investigated. It showed that in the range of NaCl concentration of 0.05-0.5 mol/L, pH 6-10, temperature 25-55 degrees C and flow rate of 0.20-0.80 mL/min, the adsorption efficiency was over 90%.
关键词[WOS]: PYROGEN ADSORPTION ;  LIPOPOLYSACCHARIDE ;  ADSORBENTS
语种: 英语
WOS记录号: WOS:000237325400005
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/140247
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Chinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatog Res & Anal Ctr, Dalian 116023, Peoples R China

Recommended Citation:
Tang, SW,Kong, L,Ou, JJ,et al. Preparation of affinity matrices for endotoxin removal[J]. CHINESE JOURNAL OF ANALYTICAL CHEMISTRY,2006,34(4):455-458.
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