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题名: Immunoassay of P-glycoprotein on single cell by capillary electrophoresis with laser induced fluorescence detection
作者: Xiao, H;  Li, X;  Zou, HF;  Yang, L;  Yang, YQ;  Wang, YL;  Wang, HL;  Le, XC
关键词: capillary electrophoresis ;  laser induced fluorescence detection ;  mulitidrug resistance ;  beta-glycoprotein ;  resistance factor
刊名: ANALYTICA CHIMICA ACTA
发表日期: 2006-01-25
DOI: 10.1016/j.aca.2005.09.051
卷: 556, 期:2, 页:340-346
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology ;  Physical Sciences
类目[WOS]: Chemistry, Analytical
研究领域[WOS]: Chemistry
英文摘要: The cellular mechanism based on P-glycoprotein (PGP) for its drug pump function has become very important in multidrug resistance (MDR) research. A method has been established to characterize PGP on single K562 cell by coupling capillary electrophoresis with laser induced fluorescence detection. A permeable intact cell after the immunoassay binding with fluorescence labeling antibody was injected into the capillary and directly separated without lysis. It was found that once 5-10 optional cells were detected in batch. the PGP amount oil this cell line could be outlined and calculated clearly. The PGP amount on K562 MDR cell line is 3.88 times higher than that on K562 sensitive cell line. These two cell lines with immunoassay binding were also analyzed by injection of multi-cells in order to improve the throughput, A resistance factor so called multidrug resistance multiple (MRM) was introduced to evaluate the MDR difference between cell lines. The MRM values of the cell line K562 measured by single cell analysis are well correlated with those by flow cytometry, which also prove the validity of our method in single cell analysis for the possibility of cancer diagnosis, pharmacokinetics and drug screening in future. (c) 2005 Elsevier B.V. All rights reserved.
关键词[WOS]: MULTIDRUG-RESISTANCE ;  FLOW-CYTOMETRY ;  EXPRESSION ;  CANCER ;  SENSITIVITY ;  COMBINATION ;  LEUKEMIA ;  BINDING ;  PROTEIN ;  DRUGS
语种: 英语
WOS记录号: WOS:000234729700013
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/140262
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Chinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatogr R&A Ctr, Dalian 116023, Peoples R China
2.Chinese Acad Sci, Dalian Inst Chem Phys, Lab Pharmaceut Resource Discovery, Dalian 116023, Peoples R China
3.Univ Alberta, Fac Med, Dept Publ Hlth Sci, Edmonton, AB T6G 2G3, Canada

Recommended Citation:
Xiao, H,Li, X,Zou, HF,et al. Immunoassay of P-glycoprotein on single cell by capillary electrophoresis with laser induced fluorescence detection[J]. ANALYTICA CHIMICA ACTA,2006,556(2):340-346.
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