中国科学院大连化学物理研究所机构知识库
Advanced  
DICP OpenIR  > 中国科学院大连化学物理研究所  > 期刊论文
题名: Effect of pH of chitosan solution on properties of sodium alginate-chitosan microcapsules with cells
作者: Yu, WT;  Liu, XD;  Li, XX;  Qi, WT;  Ren, DW;  Ma, XJ
关键词: chitosan ;  alginate ;  pH ;  microcapsule ;  cell culture
刊名: CHEMICAL JOURNAL OF CHINESE UNIVERSITIES-CHINESE
发表日期: 2006-01-10
卷: 27, 期:1, 页:182-186
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology ;  Physical Sciences
类目[WOS]: Chemistry, Multidisciplinary
研究领域[WOS]: Chemistry
英文摘要: Sodium alginate-chitosan (AC) microcapsules are often used as the immobilization carriers for cell culture. They are composed of porous gel as the core facilitating cell adhesion, and coated with polyelectrolyte complex membrane protecting cells and allowing selective mass transfer. Therefore, the regulation and control of membrane properties can directly affect the growth and metabolism of cells in microcapsules. Among many parameters, pH of chitosan solution is the important one. In this paper, the formation of polyelectrolyte membrane, the degree of complex formation and protein diffusion at different pH values were directly observed in situ by using a confocal laser scanning microscope(CLSM). The effect of pH on the properties of AC microcapsule membrane was discussed. The optimal pH was determined in the preparation of AC microcapsules with different cells(animal and microbial cells). It was demonstrated that the membrane thickness showed "high-low-high" shape, the swelling behavior of microcapsules "low-high-low" shape and the protein diffusion across the membrane "low-high-low "shape as a function of pH, the inflection points all appeared at pH = 4.00 and 5.50. Combining with the sensitivity experiment of different kinds of cells to environment pH, it was suggested that the optimal pH of chitosan solution for AC microencapsulated animal cells was 5.50, AC microencapsulated E. coli cells 5.00, and AC microencapsulated yeast cells such as saccharomyces cerevisiae (S. cere.) 4.50, respectively.
关键词[WOS]: BEHAVIOR ;  BEADS
语种: 英语
WOS记录号: WOS:000234815500047
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/140267
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

Files in This Item:

There are no files associated with this item.


作者单位: 1.Chinese Acad Sci, Dalian Inst Chem Phys, Lab Biomed Mat Engn, Dalian 116023, Peoples R China
2.Sichuan Univ, Coll Mat Sci & Engn, Chengdu 610064, Peoples R China

Recommended Citation:
Yu, WT,Liu, XD,Li, XX,et al. Effect of pH of chitosan solution on properties of sodium alginate-chitosan microcapsules with cells[J]. CHEMICAL JOURNAL OF CHINESE UNIVERSITIES-CHINESE,2006,27(1):182-186.
Service
 Recommend this item
 Sava as my favorate item
 Show this item's statistics
 Export Endnote File
Google Scholar
 Similar articles in Google Scholar
 [Yu, WT]'s Articles
 [Liu, XD]'s Articles
 [Li, XX]'s Articles
CSDL cross search
 Similar articles in CSDL Cross Search
 [Yu, WT]‘s Articles
 [Liu, XD]‘s Articles
 [Li, XX]‘s Articles
Related Copyright Policies
Null
Social Bookmarking
  Add to CiteULike  Add to Connotea  Add to Del.icio.us  Add to Digg  Add to Reddit 
所有评论 (0)
暂无评论
 
评注功能仅针对注册用户开放,请您登录
您对该条目有什么异议,请填写以下表单,管理员会尽快联系您。
内 容:
Email:  *
单位:
验证码:   刷新
您在IR的使用过程中有什么好的想法或者建议可以反馈给我们。
标 题:
 *
内 容:
Email:  *
验证码:   刷新

Items in IR are protected by copyright, with all rights reserved, unless otherwise indicated.

 

 

Valid XHTML 1.0!
Powered by CSpace