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题名: Liquid chromatography-mass spectrometric analysis of puerarin and its metabolite in human urine
作者: Tian, Hongzhe;  Yang, Bingcheng;  Xu, Jing;  Wang, Hua;  Guan, Yafeng
关键词: capillary LC-MS ;  daidzein ;  isoflavonoid ;  pueraria radix ;  puerarin
刊名: JOURNAL OF SEPARATION SCIENCE
发表日期: 2006-11-01
DOI: 10.1002/jssc.200600033
卷: 29, 期:17, 页:2692-2696
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology ;  Physical Sciences
类目[WOS]: Chemistry, Analytical
研究领域[WOS]: Chemistry
英文摘要: A specific LG-MS method was developed that allowed simultaneous determination of puerarin (PU) and its major metabolite, daidzein (DA), in human urine samples. PU and DA were separated on a packed capillary ODS column with on column concentration. Identification and quantification of the analytes were performed with ESI-Q-TOF mass spectroscopy in negative ionization mode. The method was validated, yielding calibration curves with correlation coefficients greater than 0.998. The LOQ for PU and DA from human urine samples was 0.1 and 0.05 nmol/mL, respectively. Assay accuracy and precision of quality control samples were within +/- 15%. Recoveries of PU and DA in spiked samples were in the range of 79.6-90.4 and 82.3-92.4%, respectively.
关键词[WOS]: TIME-RESOLVED FLUOROIMMUNOASSAY ;  ELECTROCHEMICAL DETECTION ;  CAPILLARY-ELECTROPHORESIS ;  QUANTITATIVE-ANALYSIS ;  DAIDZEIN ;  GENISTEIN ;  ISOFLAVONES ;  PERFORMANCE ;  PLASMA ;  SOY
语种: 英语
WOS记录号: WOS:000243338600017
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/140270
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Chinese Acad Sci, Dalian Inst Chem Phys, Dept Instrumentat & Analyt Chem, Dalian 116023, Peoples R China

Recommended Citation:
Tian, Hongzhe,Yang, Bingcheng,Xu, Jing,et al. Liquid chromatography-mass spectrometric analysis of puerarin and its metabolite in human urine[J]. JOURNAL OF SEPARATION SCIENCE,2006,29(17):2692-2696.
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