中国科学院大连化学物理研究所机构知识库
Advanced  
DICP OpenIR  > 中国科学院大连化学物理研究所  > 期刊论文
题名: Optimization of recombinant cytochrome p450 2C9 protein production in Escherichia coli DH5 alpha by statistically-based experimental design
作者: Hao, Da C.;  Zhu, Pan H.;  Yang, Sheng L.;  Yang, Ling
关键词: cytochrome P4502C9 ;  heterologous expression ;  optimization ;  Taguchi orthogonal design ;  desirability function ;  central composite design
刊名: WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY
发表日期: 2006-11-01
DOI: 10.1007/s11274-006-9158-9
卷: 22, 期:11, 页:1169-1176
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology ;  Life Sciences & Biomedicine
类目[WOS]: Biotechnology & Applied Microbiology
研究领域[WOS]: Biotechnology & Applied Microbiology
英文摘要: Cytochrome P450 (CYP) 2C9 is of major importance in drug metabolism. However, the low yield of recombinant CYP2C9 protein in E. coli strains prevents its extensive use in the study of in vitro drug metabolism. In the present study, Taguchi design and desirability function were first used to investigate the effect of medium components (glycerol, delta-ALA, IPTG, ampicillin, chloramphenicol, inoculum density, peptone, thiamine, trace elements, NH4Cl, and MgSO4) on recombinant human CYP2C9 production by E. coli DH5 alpha. An L-12 (2(11)) orthogonal array was used to design the experiments to screen out the most influential factors. The CYP concentration and the specific content of CYP were considered as two product quality variables. A desirability function was applied to combine these two qualities as a single objective function. Optimization via central composite design (CCD) was then undertaken to yield the best performance. The confirmation experiments indicated that the expression performance under the optimized conditions was better than those obtained under other conditions. A compromise between conflicting goals, such as achievement of good yield of recombinant CYP2C9 and facility of the following purification, was found by means of the desirability function D. This is the first report that combined Taguchi design and CCD, and performed experiments in a multiresponse framework to optimize the production of human CYP in a recombinant E. coli strain.
关键词[WOS]: RESPONSE-SURFACE METHODOLOGY ;  FERMENTATION CONDITIONS ;  DESIRABILITY FUNCTION ;  MEDIUM CONSTITUENTS ;  ETHANOL ;  RESOLUTION ;  EXPRESSION ;  CYP2C9
语种: 英语
WOS记录号: WOS:000241905000009
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/140272
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

Files in This Item:

There are no files associated with this item.


作者单位: 1.Chinese Acad Sci, Dalian Inst Chem Phys, Lab Pharmaceut Resource Discovery, Dalian 116023, Peoples R China
2.Chinese Acad Sci, Shanghai Res Ctr Biotechnol, Shanghai, Peoples R China
3.Chinese Acad Sci, Grad Sch, Beijing, Peoples R China

Recommended Citation:
Hao, Da C.,Zhu, Pan H.,Yang, Sheng L.,et al. Optimization of recombinant cytochrome p450 2C9 protein production in Escherichia coli DH5 alpha by statistically-based experimental design[J]. WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY,2006,22(11):1169-1176.
Service
 Recommend this item
 Sava as my favorate item
 Show this item's statistics
 Export Endnote File
Google Scholar
 Similar articles in Google Scholar
 [Hao, Da C.]'s Articles
 [Zhu, Pan H.]'s Articles
 [Yang, Sheng L.]'s Articles
CSDL cross search
 Similar articles in CSDL Cross Search
 [Hao, Da C.]‘s Articles
 [Zhu, Pan H.]‘s Articles
 [Yang, Sheng L.]‘s Articles
Related Copyright Policies
Null
Social Bookmarking
  Add to CiteULike  Add to Connotea  Add to Del.icio.us  Add to Digg  Add to Reddit 
所有评论 (0)
暂无评论
 
评注功能仅针对注册用户开放,请您登录
您对该条目有什么异议,请填写以下表单,管理员会尽快联系您。
内 容:
Email:  *
单位:
验证码:   刷新
您在IR的使用过程中有什么好的想法或者建议可以反馈给我们。
标 题:
 *
内 容:
Email:  *
验证码:   刷新

Items in IR are protected by copyright, with all rights reserved, unless otherwise indicated.

 

 

Valid XHTML 1.0!
Powered by CSpace