中国科学院大连化学物理研究所机构知识库
Advanced  
DICP OpenIR  > 中国科学院大连化学物理研究所  > 期刊论文
题名: Rapid protein digestion and identification using monolithic enzymatic microreactor coupled with nano-liquid chromatography-electro spray ionization mass spectrometry
作者: Duan, JC;  Sun, LL;  Liang, Z;  Zhang, J;  Wang, H;  Zhang, LH;  Zhang, WB;  Zhang, YK
关键词: monolithic column ;  enzymatic reactor ;  protein identification ;  nano-liquid chromatography-tandem mass spectrometry
刊名: JOURNAL OF CHROMATOGRAPHY A
发表日期: 2006-02-17
DOI: 10.1016/j.chroma.2005.11.102
卷: 1106, 期:1-2, 页:165-174
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology ;  Life Sciences & Biomedicine ;  Physical Sciences
类目[WOS]: Biochemical Research Methods ;  Chemistry, Analytical
研究领域[WOS]: Biochemistry & Molecular Biology ;  Chemistry
英文摘要: A novel monolithic enzymatic microreactor was prepared in the fused-silica capillary by in situ polymerization of acrylamide (AA), Nacryloxysuccinimide (NAS) and ethylene dimethacrylate (EDMA) in the presence of a binary porogenic mixture of dodecanol and cyclohexanol, which could offer very low back pressure, enabling the fast digestion of proteins. The performance of the monolithic microreactor was demonstrated by digesting cytochrome c at high flow rate, and the comparisons between the in-solution digestion and on-column reaction were made by a nano-high performance liquid chromatography-mass spectrometry (nano-fIPLC-MS) system. The performance of the monolithic microreactor was demonostrated with the digestion of cytochrome c at the fast flow rate of 1 mu L-min, which afforded a residence time of 7 s, yielding a sequence coverage of 54.81% using strict multiple database searching thresholds. Furture more, a mixture of four standard proteins was digested and analyzed using the on-line digestion and nano-HPLC-MS system. The results showed the promising of such a system in the analysis of protein mixture. (c) 2005 Elsevier B.V. All rights reserved.
关键词[WOS]: POROUS POLYMER MONOLITHS ;  CAPILLARY-ELECTROPHORESIS ;  PROTEOLYTIC DIGESTION ;  TRYPSIN MICROREACTOR ;  PEPTIDE SEPARATION ;  STATIONARY PHASES ;  SOL-GEL ;  ONLINE ;  PROTEOMICS ;  CHIP
语种: 英语
WOS记录号: WOS:000235256800028
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/140278
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

Files in This Item:

There are no files associated with this item.


作者单位: 1.Chinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatog Res & Anal Ctr, Dalian 116023, Peoples R China

Recommended Citation:
Duan, JC,Sun, LL,Liang, Z,et al. Rapid protein digestion and identification using monolithic enzymatic microreactor coupled with nano-liquid chromatography-electro spray ionization mass spectrometry[J]. JOURNAL OF CHROMATOGRAPHY A,2006,1106(1-2):165-174.
Service
 Recommend this item
 Sava as my favorate item
 Show this item's statistics
 Export Endnote File
Google Scholar
 Similar articles in Google Scholar
 [Duan, JC]'s Articles
 [Sun, LL]'s Articles
 [Liang, Z]'s Articles
CSDL cross search
 Similar articles in CSDL Cross Search
 [Duan, JC]‘s Articles
 [Sun, LL]‘s Articles
 [Liang, Z]‘s Articles
Related Copyright Policies
Null
Social Bookmarking
  Add to CiteULike  Add to Connotea  Add to Del.icio.us  Add to Digg  Add to Reddit 
所有评论 (0)
暂无评论
 
评注功能仅针对注册用户开放,请您登录
您对该条目有什么异议,请填写以下表单,管理员会尽快联系您。
内 容:
Email:  *
单位:
验证码:   刷新
您在IR的使用过程中有什么好的想法或者建议可以反馈给我们。
标 题:
 *
内 容:
Email:  *
验证码:   刷新

Items in IR are protected by copyright, with all rights reserved, unless otherwise indicated.

 

 

Valid XHTML 1.0!
Powered by CSpace