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题名: Capillary electrochromatography with zwitterionic stationary phase on the lysine-bonded poly(glycidyl methacrylate-co-ethylene dimethacrylate) monolithic capillary column
作者: Dong, Xiaoli;  Dong, Jing;  Ou, Junjie;  Zhu, Yan;  Zou, Hanfa
关键词: aromatic acids ;  basic pharmaceuticals ;  capillary electrochromatography ;  lysine-boncled zwitterion ;  methacrylate-based monolith
刊名: ELECTROPHORESIS
发表日期: 2006-06-01
DOI: 10.1002/elps.200500865
卷: 27, 期:12, 页:2518-2525
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology ;  Life Sciences & Biomedicine ;  Physical Sciences
类目[WOS]: Biochemical Research Methods ;  Chemistry, Analytical
研究领域[WOS]: Biochemistry & Molecular Biology ;  Chemistry
英文摘要: A polymer-based neutral monolithic capillary column was prepared by radical polymerization of glycidyl methacrylate and ethylene dimethacrylate in a 100 lam id fused-silica capillary, and the prepared monolithic column was subsequently modified based on a ring opening reaction of epoxide groups with 1 M lysine in solution (pH 8.0) at 75 degrees C for 10 h to produce a lysine chemically bonded stationary phases in capillary column. The ring opening reaction conditions were optimized so that the column could generate substantial EOF Due to the zwitterionic functional groups of the lysine covalently bonded on the polymer monolithic rod, the prepared column can generate cathodic and anodic EOF by varying the pH values of running buffer during CEC separation. EOF reached the maximum of -2.0 x 10(-8) m(2)v(-1)s(-1) and 2.6 x 10(-8) m(2)v(-1)s(-1) with pH of the running buffer of 2.25 and 10, respectively. As a consequence, neutral compounds, ionic solutes such as phenols, aromatic acids, anilines, and basic pharmaceuticals were all successfully separated on the column by CEC. Hydrophobic interaction is responsible for separation of neutral analytes. In addition, the electrostatic and hydrophobic interaction and the electrophoretic migration play a significant role in separation of the ionic or ionizable analytes.
关键词[WOS]: LIQUID-CHROMATOGRAPHY ;  SILICA COLUMN ;  AMINO-ACIDS ;  SEPARATION ;  EXCHANGE ;  ELECTROPHORESIS ;  CONSTANTS ;  DRIVEN ;  FLOW
语种: 英语
WOS记录号: WOS:000238899400025
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/140280
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Chinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatog Res & Anal Ctr, Dalian 116023, Peoples R China
2.Zhejiang Univ, Dept Chem, Hangzhou 310027, Peoples R China

Recommended Citation:
Dong, Xiaoli,Dong, Jing,Ou, Junjie,et al. Capillary electrochromatography with zwitterionic stationary phase on the lysine-bonded poly(glycidyl methacrylate-co-ethylene dimethacrylate) monolithic capillary column[J]. ELECTROPHORESIS,2006,27(12):2518-2525.
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