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Capillary electrochromatography with zwitterionic stationary phase on the lysine-bonded poly(glycidyl methacrylate-co-ethylene dimethacrylate) monolithic capillary column
Dong, Xiaoli; Dong, Jing; Ou, Junjie; Zhu, Yan; Zou, Hanfa
关键词Aromatic Acids Basic Pharmaceuticals Capillary Electrochromatography Lysine-boncled Zwitterion Methacrylate-based Monolith
刊名ELECTROPHORESIS
2006-06-01
DOI10.1002/elps.200500865
27期:12页:2518-2525
收录类别SCI
文章类型Article
WOS标题词Science & Technology ; Life Sciences & Biomedicine ; Physical Sciences
类目[WOS]Biochemical Research Methods ; Chemistry, Analytical
研究领域[WOS]Biochemistry & Molecular Biology ; Chemistry
关键词[WOS]LIQUID-CHROMATOGRAPHY ; SILICA COLUMN ; AMINO-ACIDS ; SEPARATION ; EXCHANGE ; ELECTROPHORESIS ; CONSTANTS ; DRIVEN ; FLOW
英文摘要A polymer-based neutral monolithic capillary column was prepared by radical polymerization of glycidyl methacrylate and ethylene dimethacrylate in a 100 lam id fused-silica capillary, and the prepared monolithic column was subsequently modified based on a ring opening reaction of epoxide groups with 1 M lysine in solution (pH 8.0) at 75 degrees C for 10 h to produce a lysine chemically bonded stationary phases in capillary column. The ring opening reaction conditions were optimized so that the column could generate substantial EOF Due to the zwitterionic functional groups of the lysine covalently bonded on the polymer monolithic rod, the prepared column can generate cathodic and anodic EOF by varying the pH values of running buffer during CEC separation. EOF reached the maximum of -2.0 x 10(-8) m(2)v(-1)s(-1) and 2.6 x 10(-8) m(2)v(-1)s(-1) with pH of the running buffer of 2.25 and 10, respectively. As a consequence, neutral compounds, ionic solutes such as phenols, aromatic acids, anilines, and basic pharmaceuticals were all successfully separated on the column by CEC. Hydrophobic interaction is responsible for separation of neutral analytes. In addition, the electrostatic and hydrophobic interaction and the electrophoretic migration play a significant role in separation of the ionic or ionizable analytes.
语种英语
WOS记录号WOS:000238899400025
引用统计
被引频次:44[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://cas-ir.dicp.ac.cn/handle/321008/140280
专题中国科学院大连化学物理研究所
作者单位1.Chinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatog Res & Anal Ctr, Dalian 116023, Peoples R China
2.Zhejiang Univ, Dept Chem, Hangzhou 310027, Peoples R China
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GB/T 7714
Dong, Xiaoli,Dong, Jing,Ou, Junjie,et al. Capillary electrochromatography with zwitterionic stationary phase on the lysine-bonded poly(glycidyl methacrylate-co-ethylene dimethacrylate) monolithic capillary column[J]. ELECTROPHORESIS,2006,27(12):2518-2525.
APA Dong, Xiaoli,Dong, Jing,Ou, Junjie,Zhu, Yan,&Zou, Hanfa.(2006).Capillary electrochromatography with zwitterionic stationary phase on the lysine-bonded poly(glycidyl methacrylate-co-ethylene dimethacrylate) monolithic capillary column.ELECTROPHORESIS,27(12),2518-2525.
MLA Dong, Xiaoli,et al."Capillary electrochromatography with zwitterionic stationary phase on the lysine-bonded poly(glycidyl methacrylate-co-ethylene dimethacrylate) monolithic capillary column".ELECTROPHORESIS 27.12(2006):2518-2525.
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