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Capillary electrochromatography with zwitterionic stationary phase on the lysine-bonded poly(glycidyl methacrylate-co-ethylene dimethacrylate) monolithic capillary column
Dong, Xiaoli; Dong, Jing; Ou, Junjie; Zhu, Yan; Zou, Hanfa
KeywordAromatic Acids Basic Pharmaceuticals Capillary Electrochromatography Lysine-boncled Zwitterion Methacrylate-based Monolith
Source PublicationELECTROPHORESIS
2006-06-01
DOI10.1002/elps.200500865
Volume27Issue:12Pages:2518-2525
Indexed BySCI
SubtypeArticle
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine ; Physical Sciences
WOS SubjectBiochemical Research Methods ; Chemistry, Analytical
WOS Research AreaBiochemistry & Molecular Biology ; Chemistry
WOS KeywordLIQUID-CHROMATOGRAPHY ; SILICA COLUMN ; AMINO-ACIDS ; SEPARATION ; EXCHANGE ; ELECTROPHORESIS ; CONSTANTS ; DRIVEN ; FLOW
AbstractA polymer-based neutral monolithic capillary column was prepared by radical polymerization of glycidyl methacrylate and ethylene dimethacrylate in a 100 lam id fused-silica capillary, and the prepared monolithic column was subsequently modified based on a ring opening reaction of epoxide groups with 1 M lysine in solution (pH 8.0) at 75 degrees C for 10 h to produce a lysine chemically bonded stationary phases in capillary column. The ring opening reaction conditions were optimized so that the column could generate substantial EOF Due to the zwitterionic functional groups of the lysine covalently bonded on the polymer monolithic rod, the prepared column can generate cathodic and anodic EOF by varying the pH values of running buffer during CEC separation. EOF reached the maximum of -2.0 x 10(-8) m(2)v(-1)s(-1) and 2.6 x 10(-8) m(2)v(-1)s(-1) with pH of the running buffer of 2.25 and 10, respectively. As a consequence, neutral compounds, ionic solutes such as phenols, aromatic acids, anilines, and basic pharmaceuticals were all successfully separated on the column by CEC. Hydrophobic interaction is responsible for separation of neutral analytes. In addition, the electrostatic and hydrophobic interaction and the electrophoretic migration play a significant role in separation of the ionic or ionizable analytes.
Language英语
WOS IDWOS:000238899400025
Citation statistics
Cited Times:45[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://cas-ir.dicp.ac.cn/handle/321008/140280
Collection中国科学院大连化学物理研究所
Affiliation1.Chinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatog Res & Anal Ctr, Dalian 116023, Peoples R China
2.Zhejiang Univ, Dept Chem, Hangzhou 310027, Peoples R China
Recommended Citation
GB/T 7714
Dong, Xiaoli,Dong, Jing,Ou, Junjie,et al. Capillary electrochromatography with zwitterionic stationary phase on the lysine-bonded poly(glycidyl methacrylate-co-ethylene dimethacrylate) monolithic capillary column[J]. ELECTROPHORESIS,2006,27(12):2518-2525.
APA Dong, Xiaoli,Dong, Jing,Ou, Junjie,Zhu, Yan,&Zou, Hanfa.(2006).Capillary electrochromatography with zwitterionic stationary phase on the lysine-bonded poly(glycidyl methacrylate-co-ethylene dimethacrylate) monolithic capillary column.ELECTROPHORESIS,27(12),2518-2525.
MLA Dong, Xiaoli,et al."Capillary electrochromatography with zwitterionic stationary phase on the lysine-bonded poly(glycidyl methacrylate-co-ethylene dimethacrylate) monolithic capillary column".ELECTROPHORESIS 27.12(2006):2518-2525.
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