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题名: Culturable actinobacteria from the marine sponge Hymeniacidon perleve: isolation and phylogenetic diversity by 16S rRNA gene-RFLP analysis
作者: Zhang, Haitao;  Lee, Yoo Kyung;  Zhang, Wei;  Lee, Hong Kum
关键词: marine sponge ;  actinobacteria ;  RFLP ;  Streptomycetes ;  Nocardiopsis ;  Pseudonocardia ;  Nocardia ;  Micromonospora ;  Rhodococcus ;  Actinoalloteichus
刊名: ANTONIE VAN LEEUWENHOEK INTERNATIONAL JOURNAL OF GENERAL AND MOLECULAR MICROBIOLOGY
发表日期: 2006-08-01
DOI: 10.1007/s10482-006-9070-1
卷: 90, 期:2, 页:159-169
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology ;  Life Sciences & Biomedicine
类目[WOS]: Microbiology
研究领域[WOS]: Microbiology
英文摘要: A total of 106 actinobacteria associated with the marine sponge Hymeniacidon perleve collected from the Yellow Sea, China were isolated using eight different media. The number of species and genera of actinobacteria recovered from the different media varied significantly, underlining the importance of optimizing the isolation conditions. The phylogenetic diversity of the actinobacteria isolates was assessed using 16S rRNA gene amplification-restriction fragment length polymorphism (RFLP) analysis of the 106 strains with different morphologies. The RFLP fingerprinting of selected strains by HhaI-digestion of the 16S rRNA genes resulted in 11 different patterns. The HhaI-RFLP analysis gave good resolution for the identification of the actinobacteria isolates at the genus level. A phylogenetic analysis using 16S rRNA gene sequences revealed that the isolates belonged to seven genera of culturable actinobacteria including Actinoalloteichus, Micromonospora, Nocardia, Nocardiopsis, Pseudonocardia, Rhodococcus, and Streptomyces. The dominant genus was Streptomyces, which represented 74% of the isolates. Three of the strains identified are candidates for new species.
关键词[WOS]: NATURAL-PRODUCTS ;  RHOPALOEIDES ODORABILE ;  APLYSINA-CAVERNICOLA ;  MICROBIAL COMMUNITY ;  SP-NOV. ;  SEDIMENTS ;  BACTERIA ;  METABOLITES ;  WIDESPREAD ;  SEA
语种: 英语
WOS记录号: WOS:000239955700005
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/140287
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Chinese Acad Sci, Dalian Inst Chem Phys, Marine Bioprod Engn Grp, Dalian 116023, Peoples R China
2.KORDI, KOPRI, Polar BioCtr, Seoul 425600, South Korea
3.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China

Recommended Citation:
Zhang, Haitao,Lee, Yoo Kyung,Zhang, Wei,et al. Culturable actinobacteria from the marine sponge Hymeniacidon perleve: isolation and phylogenetic diversity by 16S rRNA gene-RFLP analysis[J]. ANTONIE VAN LEEUWENHOEK INTERNATIONAL JOURNAL OF GENERAL AND MOLECULAR MICROBIOLOGY,2006,90(2):159-169.
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