中国科学院大连化学物理研究所机构知识库
Advanced  
DICP OpenIR  > 中国科学院大连化学物理研究所  > 期刊论文
题名: Preparation of protein-loaded microspheres with size <= 10 mu m by electrostatic droplet generation technology
作者: Xue, WM;  Liu, XD;  Yu, WT;  Ma, XJ
关键词: electrostatic droplet generation technology ;  insulin ;  hemoglobin ;  alginate ;  microsphere ;  size ;  encapsulation efficiency
刊名: CHINESE SCIENCE BULLETIN
发表日期: 2006-02-01
DOI: 10.1007/s11434-006-0279-1
卷: 51, 期:3, 页:279-286
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Multidisciplinary Sciences
研究领域[WOS]: Science & Technology - Other Topics
英文摘要: The development of non-injection route for protein drugs, especially oral administration, has been the main focus of controlled release of drugs. To overcome obstacles unsolved such as enzyme degradation and penetration barrier of intestinal epithelium, technologies using microspheres as carrier of protein drugs have been proven potential to realize oral administration. It has been demonstrated that microspheres can not only protect proteins, but also facilitate the penetration and absorption through Peyer's patches when the size is smaller than 10 mu m. Therefore, the objective of this paper is to prepare protein-loaded microspheres with size <= 10 mu m. Electrostatic droplet generation technology was used with insulin and hemoglobin as drug models and sodium alginate as microsphere material. By decreasing the surface tension of feed solution by adding surfactant, and improving electric field distribution by changing the shape of container and electrode for gelation solution, protein-loaded microspheres with mean size less than 10 mu m were successfully produced through needle with diameter of 400 pm. The microspheres showed good sphericity and narrow size distribution. The mean standard variance of size distribution was 1.61. The encapsulation efficiency of proteins was over 70%. Moreover, the significance analysis of factors influencing the size of protein loaded microspheres was carried out through orthogonal experiments, which showed that output voltage (U), needle diameter (D) and the distance between needle tips to the surface of gelation solution (delta) influenced significantly the size of micro-spheres. Finally, the statistic analysis showed that when confidence level was alpha=0.05, and alpha=0.1, confidence interval of microsphere size can be (6.2545, 10.1735) and (6.6022, 9.8258) correspondingly, suggesting that there is good repeatability and reliability for improving electrostatic droplet generation technology to prepare protein-loaded microspheres with size <= 10 mu m.
关键词[WOS]: ORAL DELIVERY ;  MICROBEADS ;  PEPTIDE ;  DRUGS
语种: 英语
WOS记录号: WOS:000236651200004
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/140290
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

Files in This Item:

There are no files associated with this item.


作者单位: 1.NW Univ Xian, Coll Chem Engn, Xian 710069, Peoples R China
2.Chinese Acad Sci, Dalian Inst Chem Phys, Dept Biotechnol, Dalian 116023, Peoples R China

Recommended Citation:
Xue, WM,Liu, XD,Yu, WT,et al. Preparation of protein-loaded microspheres with size <= 10 mu m by electrostatic droplet generation technology[J]. CHINESE SCIENCE BULLETIN,2006,51(3):279-286.
Service
 Recommend this item
 Sava as my favorate item
 Show this item's statistics
 Export Endnote File
Google Scholar
 Similar articles in Google Scholar
 [Xue, WM]'s Articles
 [Liu, XD]'s Articles
 [Yu, WT]'s Articles
CSDL cross search
 Similar articles in CSDL Cross Search
 [Xue, WM]‘s Articles
 [Liu, XD]‘s Articles
 [Yu, WT]‘s Articles
Related Copyright Policies
Null
Social Bookmarking
  Add to CiteULike  Add to Connotea  Add to Del.icio.us  Add to Digg  Add to Reddit 
所有评论 (0)
暂无评论
 
评注功能仅针对注册用户开放,请您登录
您对该条目有什么异议,请填写以下表单,管理员会尽快联系您。
内 容:
Email:  *
单位:
验证码:   刷新
您在IR的使用过程中有什么好的想法或者建议可以反馈给我们。
标 题:
 *
内 容:
Email:  *
验证码:   刷新

Items in IR are protected by copyright, with all rights reserved, unless otherwise indicated.

 

 

Valid XHTML 1.0!
Powered by CSpace