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题名: Proliferation, viability, and metabolism of human tumor and normal cells cultured in microcapsule
作者: Zhang, Xulang;  Wang, Wei;  Xie, Yubing;  Zhang, Ying;  Wang, Xiuli;  Guo, Xin;  Ma, Xiaojun
关键词: alginate ;  microcapsule ;  cell encapsulation ;  poly-L-lysine ;  tumor cell
刊名: APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY
发表日期: 2006-07-01
卷: 134, 期:1, 页:61-76
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology ;  Life Sciences & Biomedicine
类目[WOS]: Biochemistry & Molecular Biology ;  Biotechnology & Applied Microbiology
研究领域[WOS]: Biochemistry & Molecular Biology ;  Biotechnology & Applied Microbiology
英文摘要: In this study, we investigated the effect of the microenvironment provided by alginate-poly-L-lysine-alginate (APA) microcapsule with liquefied or gelled core on the proliferation, viability, and metabolism of human cells, including anchorage-dependent MCF-7 breast cancer cells and primary fibroblasts, and anchorage-independent K-562 leukemia cells; cells in conventional culture were used as control. The growth pattern of cells in microcapsule was examined by phase-contrast micrography. The cell viability, proliferation, organization, and gene expression were evaluated by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide assay, hematoxylin and eosin staining, live/dead staining, 5-bromo-20-deoxyuridine labeling, and immunohistochemistry, respectively. Cell metabolism was determined by measuring glucose and lactate concentrations in medium. The results demonstrate that APA microcapsule with liquefied core provides a microenvironment for both anchorage-dependent and anchorage-independent cells to grow into a large cell aggregate and maintain cell viability at a constant level for a period of time. In conclusion, cells in APA microcapsule are alive and have proliferation potential with lower metabolism rate. APA microcapsule may be a useful tool for in vitro tumor cell modeling and anticancer drug screening as well as for cancer gene therapy. In addition, it lays a solid foundation for the use of microencapsulation in cell culture in vitro and cell implantation in vivo.
关键词[WOS]: IN-VITRO ;  MICROENCAPSULATION ;  THERAPY ;  ISLETS
语种: 英语
WOS记录号: WOS:000239041300006
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/140321
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Chinese Acad Sci, Dalian Inst Chem Phys, Lab Biomed Mat Engn, Dalian 116023, Peoples R China
2.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China

Recommended Citation:
Zhang, Xulang,Wang, Wei,Xie, Yubing,et al. Proliferation, viability, and metabolism of human tumor and normal cells cultured in microcapsule[J]. APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY,2006,134(1):61-76.
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