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Proliferation, viability, and metabolism of human tumor and normal cells cultured in microcapsule
Zhang, Xulang; Wang, Wei; Xie, Yubing; Zhang, Ying; Wang, Xiuli; Guo, Xin; Ma, Xiaojun
关键词Alginate Microcapsule Cell Encapsulation Poly-l-lysine Tumor Cell
刊名APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY
2006-07-01
134期:1页:61-76
收录类别SCI
文章类型Article
WOS标题词Science & Technology ; Life Sciences & Biomedicine
类目[WOS]Biochemistry & Molecular Biology ; Biotechnology & Applied Microbiology
研究领域[WOS]Biochemistry & Molecular Biology ; Biotechnology & Applied Microbiology
关键词[WOS]IN-VITRO ; MICROENCAPSULATION ; THERAPY ; ISLETS
英文摘要In this study, we investigated the effect of the microenvironment provided by alginate-poly-L-lysine-alginate (APA) microcapsule with liquefied or gelled core on the proliferation, viability, and metabolism of human cells, including anchorage-dependent MCF-7 breast cancer cells and primary fibroblasts, and anchorage-independent K-562 leukemia cells; cells in conventional culture were used as control. The growth pattern of cells in microcapsule was examined by phase-contrast micrography. The cell viability, proliferation, organization, and gene expression were evaluated by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide assay, hematoxylin and eosin staining, live/dead staining, 5-bromo-20-deoxyuridine labeling, and immunohistochemistry, respectively. Cell metabolism was determined by measuring glucose and lactate concentrations in medium. The results demonstrate that APA microcapsule with liquefied core provides a microenvironment for both anchorage-dependent and anchorage-independent cells to grow into a large cell aggregate and maintain cell viability at a constant level for a period of time. In conclusion, cells in APA microcapsule are alive and have proliferation potential with lower metabolism rate. APA microcapsule may be a useful tool for in vitro tumor cell modeling and anticancer drug screening as well as for cancer gene therapy. In addition, it lays a solid foundation for the use of microencapsulation in cell culture in vitro and cell implantation in vivo.
语种英语
WOS记录号WOS:000239041300006
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被引频次:14[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://cas-ir.dicp.ac.cn/handle/321008/140321
专题中国科学院大连化学物理研究所
作者单位1.Chinese Acad Sci, Dalian Inst Chem Phys, Lab Biomed Mat Engn, Dalian 116023, Peoples R China
2.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China
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Zhang, Xulang,Wang, Wei,Xie, Yubing,et al. Proliferation, viability, and metabolism of human tumor and normal cells cultured in microcapsule[J]. APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY,2006,134(1):61-76.
APA Zhang, Xulang.,Wang, Wei.,Xie, Yubing.,Zhang, Ying.,Wang, Xiuli.,...&Ma, Xiaojun.(2006).Proliferation, viability, and metabolism of human tumor and normal cells cultured in microcapsule.APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY,134(1),61-76.
MLA Zhang, Xulang,et al."Proliferation, viability, and metabolism of human tumor and normal cells cultured in microcapsule".APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY 134.1(2006):61-76.
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