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题名: Integrated isotachophoretic reconcentration with zone electrophoresis separation on a quartz microchip for UV detection of flavonoids
作者: Ma, Bo;  Zhou, Xiaomian;  Wang, Gang;  Huang, Huaiqing;  Dai, Zhongpeng;  Qin, Jianhua;  Lin, Bingcheng
关键词: flavonoid ;  ITP-ZE ;  on-line preconcentration ;  quartz microchip ;  UV detection
刊名: ELECTROPHORESIS
发表日期: 2006-12-01
DOI: 10.1002/elps.200600392
卷: 27, 期:24, 页:4904-4909
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology ;  Life Sciences & Biomedicine ;  Physical Sciences
类目[WOS]: Biochemical Research Methods ;  Chemistry, Analytical
研究领域[WOS]: Biochemistry & Molecular Biology ;  Chemistry
英文摘要: A quartz microchip integrated isotachophoretic (ITP) preconcentration with zone electrophoresis (ZE) separation was fabricated using a novel multi-point pressure method featured in normal temperature and lower pressure during bonding process. ITP followed by subsequential ZE of two flavonoids, quercetin and isorhamnetin on the microchip was performed consecutively on the homemade microfluidic workstation with UV detection, resulting in a decreased detectable concentration of 32-fold, compared to the ZE mode only, and their detection limits decreased down to 0.2 mu g/mL and 1.2 mu g/mL, respectively
关键词[WOS]: CAPILLARY ISOTACHOPHORESIS ;  MICROFLUIDIC DEVICES ;  DNA FRAGMENTS ;  ABSORBENCY DETECTION ;  PHENOLIC-COMPOUNDS ;  CHIP ;  PRECONCENTRATION ;  SENSITIVITY ;  PERFORMANCE ;  PROTEINS
语种: 英语
WOS记录号: WOS:000243304100005
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/140348
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Acad Sinica, Dalian Inst Chem Phys, Grad Sch, Dalian 116023, Peoples R China
2.Guangzhou Municipal Peoples Hosp 1, Dept Clin Lab, Guangzhou, Peoples R China

Recommended Citation:
Ma, Bo,Zhou, Xiaomian,Wang, Gang,et al. Integrated isotachophoretic reconcentration with zone electrophoresis separation on a quartz microchip for UV detection of flavonoids[J]. ELECTROPHORESIS,2006,27(24):4904-4909.
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