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题名: On-line parallel reversed phase two-dimensional liquid chromatography for high-throughput analysis of complex proteomic samples
作者: Wang Zhicong;  Zhang Qinghe;  Li Tong;  Zhao Zhongyi;  Zhang Weibing
关键词: two-dimensional liquid chromatography ;  interface ;  trapping column ;  proteomics ;  globin
刊名: SCIENCE IN CHINA SERIES B-CHEMISTRY
发表日期: 2006-12-01
DOI: 10.1007/s11426-006-2024-7
卷: 49, 期:6, 页:527-533
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology ;  Physical Sciences
类目[WOS]: Chemistry, Multidisciplinary
研究领域[WOS]: Chemistry
英文摘要: A comprehensive two-dimensional liquid chromatographic system (2D SCX/RP) is constructed with a 10-port-2-way valve using strong cation exchange chromatography (Hypersil SCX, 100 mm x 4.6 mm I.D.) followed by reversed phase chromatography (Hypersil BIDS C18, 15 mm x 4.6 mm I.D.) to separate the complex peptides from globin peptic hydrolysate. After the sample was loaded on the SCX column, the phosphate buffer (pH 4.0) was used to elute the peptides. Then, elutes flowed through the interface and the peptides focused on the head of the trapping columns (Hypersil BIDS C18, 15 mm x 4.6 mm I.D.) but salt passed into the waste. After the valve was switched, the samples were flushed with a backward flow into the RP analytical column. The peptides on the SCX were eluted With 12 discontinuous steps linearly increasing salt concentrations. The peptides enriched on the trapping column were desalted and separated by the RIP columns. The resolution and the resolved peaks of the 2D SCX/RP system were greatly increased and the total peak capacity reached as high as 2280.
关键词[WOS]: TANDEM MASS-SPECTROMETRY ;  PROTEIN IDENTIFICATION TECHNOLOGY ;  YEAST PROTEOME ;  SEPARATIONS
语种: 英语
WOS记录号: WOS:000244104200007
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/140352
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Chinese Acad Sci, Dalian Inst Chem Phys, Dalian 116011, Peoples R China
2.Dalian Elite Analyt Instrument Co Ltd, Dalian 116011, Peoples R China
3.China Univ Geosci, Fac Mat Sci & Chem Engn, Wuhan 430074, Peoples R China

Recommended Citation:
Wang Zhicong,Zhang Qinghe,Li Tong,et al. On-line parallel reversed phase two-dimensional liquid chromatography for high-throughput analysis of complex proteomic samples[J]. SCIENCE IN CHINA SERIES B-CHEMISTRY,2006,49(6):527-533.
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