DICP OpenIR
On-line parallel reversed phase two-dimensional liquid chromatography for high-throughput analysis of complex proteomic samples
Wang Zhicong; Zhang Qinghe; Li Tong; Zhao Zhongyi; Zhang Weibing
KeywordTwo-dimensional Liquid Chromatography Interface Trapping Column Proteomics Globin
Source PublicationSCIENCE IN CHINA SERIES B-CHEMISTRY
2006-12-01
DOI10.1007/s11426-006-2024-7
Volume49Issue:6Pages:527-533
Indexed BySCI
SubtypeArticle
WOS HeadingsScience & Technology ; Physical Sciences
WOS SubjectChemistry, Multidisciplinary
WOS Research AreaChemistry
WOS KeywordTANDEM MASS-SPECTROMETRY ; PROTEIN IDENTIFICATION TECHNOLOGY ; YEAST PROTEOME ; SEPARATIONS
AbstractA comprehensive two-dimensional liquid chromatographic system (2D SCX/RP) is constructed with a 10-port-2-way valve using strong cation exchange chromatography (Hypersil SCX, 100 mm x 4.6 mm I.D.) followed by reversed phase chromatography (Hypersil BIDS C18, 15 mm x 4.6 mm I.D.) to separate the complex peptides from globin peptic hydrolysate. After the sample was loaded on the SCX column, the phosphate buffer (pH 4.0) was used to elute the peptides. Then, elutes flowed through the interface and the peptides focused on the head of the trapping columns (Hypersil BIDS C18, 15 mm x 4.6 mm I.D.) but salt passed into the waste. After the valve was switched, the samples were flushed with a backward flow into the RP analytical column. The peptides on the SCX were eluted With 12 discontinuous steps linearly increasing salt concentrations. The peptides enriched on the trapping column were desalted and separated by the RIP columns. The resolution and the resolved peaks of the 2D SCX/RP system were greatly increased and the total peak capacity reached as high as 2280.
Language英语
WOS IDWOS:000244104200007
Citation statistics
Cited Times:2[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://cas-ir.dicp.ac.cn/handle/321008/140352
Collection中国科学院大连化学物理研究所
Affiliation1.Chinese Acad Sci, Dalian Inst Chem Phys, Dalian 116011, Peoples R China
2.Dalian Elite Analyt Instrument Co Ltd, Dalian 116011, Peoples R China
3.China Univ Geosci, Fac Mat Sci & Chem Engn, Wuhan 430074, Peoples R China
Recommended Citation
GB/T 7714
Wang Zhicong,Zhang Qinghe,Li Tong,et al. On-line parallel reversed phase two-dimensional liquid chromatography for high-throughput analysis of complex proteomic samples[J]. SCIENCE IN CHINA SERIES B-CHEMISTRY,2006,49(6):527-533.
APA Wang Zhicong,Zhang Qinghe,Li Tong,Zhao Zhongyi,&Zhang Weibing.(2006).On-line parallel reversed phase two-dimensional liquid chromatography for high-throughput analysis of complex proteomic samples.SCIENCE IN CHINA SERIES B-CHEMISTRY,49(6),527-533.
MLA Wang Zhicong,et al."On-line parallel reversed phase two-dimensional liquid chromatography for high-throughput analysis of complex proteomic samples".SCIENCE IN CHINA SERIES B-CHEMISTRY 49.6(2006):527-533.
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