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题名: Simultaneous quantification of three isoflavonoid glycosies in rabbit plasma after oral administration of Astraogalus mongholicus extract by high-performance liquid chromatography coupled with electrospray ionization tandem mass spectrometry
作者: Zhang, X.;  Sun, Y. G.;  Cheng, M. C.;  Wang, Y. Q.;  Xiao, H. B.;  Liang, X. M.
关键词: isoflavonoid glycoside ;  Astragalus mongholicus ;  liquid chromatography ;  tandem mass spectrometry ;  plasma
刊名: ANALYTICA CHIMICA ACTA
发表日期: 2007-10-29
DOI: 10.1016/j.aca.2007.09.033
卷: 602, 期:2, 页:252-258
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology ;  Physical Sciences
类目[WOS]: Chemistry, Analytical
研究领域[WOS]: Chemistry
英文摘要: A selective and sensitive liquid chromatography coupled with triple stage quadruple tandem mass spectrometry (HPLC/TSQ-MS/MS) was developed and validated for simultaneous quantification of calycosin-7-O-beta-D-glycoside (CCSG), formononetin-7-O-beta-D-glycoside (Ononin) and (6R,10R)-9,10-dimethoxypterocarpan-3-O-beta-D-glycoside (DPG) in rabbit plasma. Plasma samples were extracted with solid-phase extraction (SPE), separated on an Inertsil ODS-3 column and detected by tandem mass spectrometry with electrospray ionization (ESI) interface in positive selective reaction monitoring (SRM) mode. 3,7,8-Trimethoxy-xanthone-1-O-primaverose was used as internal standard (IS) for quantitative measurement. For each analyte, one major product ion was chosen and used for screening of it. Calibration curves were generated over the range of 2-1000 ng mL(-1) with the correlation coefficients greater than 0.99 by using a weighted (1/chi) least squares linear regression. The method had the lower limit quantification of 0.15, 0.21 and 0.19 for CCSG, Ononin and DPG, respectively, with precision less than 20%. The intra- and inter-day precisions ranged from 2.48 to 6.38% and 4.81 to 11.78% (R.S.D.%), respectively. This assay is suitable for determining the above three trace glycosides in rabbit plasma simultaneously and thus investigating the pharmacokinetics of glycosides from Astragalus mongholicus extract in rabbits. (C) 2007 Elsevier B.V. All rights reserved.
关键词[WOS]: ASTRAGALUS-MONGHOLICUS ;  RADIX-ASTRAGALI
语种: 英语
WOS记录号: WOS:000250629800013
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/140396
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Chinese Acad Sci, Dalian Inst Chem Phys, Dalian 116023, Peoples R China

Recommended Citation:
Zhang, X.,Sun, Y. G.,Cheng, M. C.,et al. Simultaneous quantification of three isoflavonoid glycosies in rabbit plasma after oral administration of Astraogalus mongholicus extract by high-performance liquid chromatography coupled with electrospray ionization tandem mass spectrometry[J]. ANALYTICA CHIMICA ACTA,2007,602(2):252-258.
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