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题名: Investigations of the interaction between 3-aminophenylboronic acid and bovine serum albumin using fluorimetry
作者: Wang Hua-Fang;  Li Wen-You;  He Xi-Wen;  Chen Lang-Xing;  Zhang Yu-Kui
关键词: 3-aminophenylboronic acid ;  bovine serum albumin ;  fluorescence quenching ;  molecularly imprinting
刊名: ACTA CHIMICA SINICA
发表日期: 2007-01-14
卷: 65, 期:1, 页:43-48
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology ;  Physical Sciences
类目[WOS]: Chemistry, Multidisciplinary
研究领域[WOS]: Chemistry
英文摘要: In order to understand the chemical mechanism and optimal conditions of the molecular imprinting, the interaction between 3-aminophenylboronic acid (APBA) and bovine serum albumin (BSA) was investigated using fluorescence quenching method with many factors, as the pH value, ionic strength and others, which maybe influence the interaction. The research results indicate that under appropriate ionic strength and pH 6.25, the fluorescence quenching shows that APBA strongly bound with the tryptophan of BSA, and the complex was formed with a molar ratio of 2 : 1. Their apparent binding constant is K-A = 1.0 x 10(11) L-2.mol(-2), so their chemical bindings between donor BSA and acceptor APBA are relatively strong. According to the results, the interaction mechanism between APBA and BSA is predicted. During following research, it will be entirely possible to separate or enrich BSA composition, maybe it could improve imprinting and eluting efficiency greatly.
关键词[WOS]: MOLECULARLY IMPRINTED POLYMERS ;  BINDING ;  SURFACE
语种: 英语
WOS记录号: WOS:000243606300008
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/140405
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Nankai Univ, Dept Chem, Tianjin 300071, Peoples R China
2.Acad Sinica, Dalian Inst Chem Phys, Dalian 116011, Peoples R China

Recommended Citation:
Wang Hua-Fang,Li Wen-You,He Xi-Wen,et al. Investigations of the interaction between 3-aminophenylboronic acid and bovine serum albumin using fluorimetry[J]. ACTA CHIMICA SINICA,2007,65(1):43-48.
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