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Investigations of the interaction between 3-aminophenylboronic acid and bovine serum albumin using fluorimetry
Wang Hua-Fang; Li Wen-You; He Xi-Wen; Chen Lang-Xing; Zhang Yu-Kui
关键词3-aminophenylboronic Acid Bovine Serum Albumin Fluorescence Quenching Molecularly Imprinting
刊名ACTA CHIMICA SINICA
2007-01-14
65期:1页:43-48
收录类别SCI
文章类型Article
WOS标题词Science & Technology ; Physical Sciences
类目[WOS]Chemistry, Multidisciplinary
研究领域[WOS]Chemistry
关键词[WOS]MOLECULARLY IMPRINTED POLYMERS ; BINDING ; SURFACE
英文摘要In order to understand the chemical mechanism and optimal conditions of the molecular imprinting, the interaction between 3-aminophenylboronic acid (APBA) and bovine serum albumin (BSA) was investigated using fluorescence quenching method with many factors, as the pH value, ionic strength and others, which maybe influence the interaction. The research results indicate that under appropriate ionic strength and pH 6.25, the fluorescence quenching shows that APBA strongly bound with the tryptophan of BSA, and the complex was formed with a molar ratio of 2 : 1. Their apparent binding constant is K-A = 1.0 x 10(11) L-2.mol(-2), so their chemical bindings between donor BSA and acceptor APBA are relatively strong. According to the results, the interaction mechanism between APBA and BSA is predicted. During following research, it will be entirely possible to separate or enrich BSA composition, maybe it could improve imprinting and eluting efficiency greatly.
语种英语
WOS记录号WOS:000243606300008
引用统计
文献类型期刊论文
条目标识符http://cas-ir.dicp.ac.cn/handle/321008/140405
专题中国科学院大连化学物理研究所
作者单位1.Nankai Univ, Dept Chem, Tianjin 300071, Peoples R China
2.Acad Sinica, Dalian Inst Chem Phys, Dalian 116011, Peoples R China
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GB/T 7714
Wang Hua-Fang,Li Wen-You,He Xi-Wen,et al. Investigations of the interaction between 3-aminophenylboronic acid and bovine serum albumin using fluorimetry[J]. ACTA CHIMICA SINICA,2007,65(1):43-48.
APA Wang Hua-Fang,Li Wen-You,He Xi-Wen,Chen Lang-Xing,&Zhang Yu-Kui.(2007).Investigations of the interaction between 3-aminophenylboronic acid and bovine serum albumin using fluorimetry.ACTA CHIMICA SINICA,65(1),43-48.
MLA Wang Hua-Fang,et al."Investigations of the interaction between 3-aminophenylboronic acid and bovine serum albumin using fluorimetry".ACTA CHIMICA SINICA 65.1(2007):43-48.
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