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题名: Capillary hydrophilic interaction chromatography/mass spectrometry for simultaneous determination of multiple neurotransmitters in primate cerebral cortex
作者: Zhang, Xiaozhe;  Rauch, Alexander;  Lee, Han;  Xiao, Hongbin;  Rainer, Gregor;  Logothetis, Nikos K.
刊名: RAPID COMMUNICATIONS IN MASS SPECTROMETRY
发表日期: 2007
DOI: 10.1002/rcm.3251
卷: 21, 期:22, 页:3621-3628
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology ;  Physical Sciences ;  Technology
类目[WOS]: Chemistry, Analytical ;  Spectroscopy
研究领域[WOS]: Chemistry ;  Spectroscopy
英文摘要: A diverse array of neurotransmitters and neuromodulators control and affect brain function. A profound understanding of the signaling pathways and the neural circuits underlying behavior is therefore likely to require the tracking of concentration changes of active neurochemicals. In the present study, we demonstrate the feasibility of a method allowing the simultaneous determination of the concentrations of six neurotransmitters: acetylcholine, serotonin, dopamine, gamma-aminobutyric acid (GABA), glutamate and aspartate, in the extracellular brain fluid (EBF). We used hydrophilic interaction chromatography (HILIC) coupled to tandem mass spectrometry (MS/MS) to analyze the EBF from the monkey brain. A push-pull sampling method was used to collect EBF from the prefrontal cortex (PFC) of conscious monkeys at flow rates in the range of low nL/min. The detection limits of acetylcholine, serotonin, dopamine, GABA, glutamate and aspartate were 0.015,0.15,0.3,1.2, 6 and 15 femtomoles, respectively, allowing us to quantitatively determine the concentrations of these six neurotransmitters simultaneously from 500 nL in vivo samples. We conclude that HILIC/MS/MS combined with the push-pull sampling method represents a sensitive technique for simultaneous monitoring of neurotransmitters from EBF samples. Copyright (C) 2007 John Wiley & Sons, Ltd.
关键词[WOS]: AMINO-ACID NEUROTRANSMITTERS ;  IONIZATION-MASS-SPECTROMETRY ;  GAMMA-AMINOBUTYRIC-ACID ;  IN-VIVO MICRODIALYSIS ;  PUSH-PULL PERFUSION ;  ELECTROSPRAY-IONIZATION ;  LIQUID-CHROMATOGRAPHY ;  POLAR COMPOUNDS ;  ACETYLCHOLINE ;  ONLINE
语种: 英语
WOS记录号: WOS:000250993100011
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/140425
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Max Planck Inst Biol Cybernet, D-72076 Tubingen, Germany
2.Chinese Acad Sci, Dalian Inst Chem Phys, Dalian 116023, Peoples R China

Recommended Citation:
Zhang, Xiaozhe,Rauch, Alexander,Lee, Han,et al. Capillary hydrophilic interaction chromatography/mass spectrometry for simultaneous determination of multiple neurotransmitters in primate cerebral cortex[J]. RAPID COMMUNICATIONS IN MASS SPECTROMETRY,2007,21(22):3621-3628.
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