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题名: Characterization of enantio selective binding of racemic natural tetrahydropalmatine to DNA by chromatographic methods
作者: Su, Xingye;  Qin, Feng;  Kong, Liang;  Ou, Junjie;  Xie, Chuanhui;  Zou, Hanfa
关键词: tetrahydropalmatine ;  enantioselectivity ;  DNA binding ;  natural products ;  chromatographic methods
刊名: JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
发表日期: 2007
DOI: 10.1016/j.jchromb.2006.07.050
卷: 845, 期:1, 页:174-179
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology ;  Life Sciences & Biomedicine ;  Physical Sciences
类目[WOS]: Biochemical Research Methods ;  Chemistry, Analytical
研究领域[WOS]: Biochemistry & Molecular Biology ;  Chemistry
英文摘要: A racemate from natural product, tetrahydropalmatine (THP), was characterized on its enantioselective binding to DNA by the chromatographic methods including microdialysis/HPLC, centrifugal ultrifiltration/HPLC and immobilized DNA affinity chromatography. It was found that its (+)-enantiomer was preferential to binding on B-form duplex DNA including calf thymus DNA, AT and GC sequence oligo DNA, as well as triplex oligo DNA. The binding constants of the THP enantiomers to ct-DNA were determined with the methods of microdialysis/HPLC and frontal affinity chromatography. In addition, the DNA structural preference of either enantiomer was evaluated with the chromatographic methods. (c) 2006 Elsevier B.V. All rights reserved.
关键词[WOS]: PERFORMANCE LIQUID-CHROMATOGRAPHY ;  AFFINITY-CHROMATOGRAPHY ;  PROTEIN INTERACTIONS ;  METAL-COMPLEXES ;  TOPOISOMERASE-I ;  MICRODIALYSIS ;  ALKALOIDS ;  LIGANDS ;  PHASES ;  HPLC
语种: 英语
WOS记录号: WOS:000243704200021
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/140426
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Chinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatog R&A Ctr, Dalian 116023, Peoples R China

Recommended Citation:
Su, Xingye,Qin, Feng,Kong, Liang,et al. Characterization of enantio selective binding of racemic natural tetrahydropalmatine to DNA by chromatographic methods[J]. JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES,2007,845(1):174-179.
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