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题名: Effects of oligochitosans on tobacco cells and role of endogenous nitric oxide burst in the resistance of tobacco to Tobacco mosaic virus
作者: Zhao, X. M.;  She, X. P.;  Yu, W.;  Liang, X. M.;  Du, Y. G.
关键词: oligochitosan ;  Tobacco mosaic virus ;  laser scanning confocal microscopy ;  nitric oxide
刊名: JOURNAL OF PLANT PATHOLOGY
发表日期: 2007-03-01
卷: 89, 期:1, 页:55-65
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology ;  Life Sciences & Biomedicine
类目[WOS]: Plant Sciences
研究领域[WOS]: Plant Sciences
英文摘要: Binding to tobacco cells of oliogochitosan labeled with the fluorophore 2-aminoacidone (2-AMAC) was investigated using laser scanning confocal. microscopy (LSCM). Production of NO in epidermal tobacco cells treated with oligochitosan was investigated by epidermal strip bioassay and LSCM, using the cell-permeable fluorophore diaminofluorescein diacetate (DAF-2D). Binding of the labeled oligochitosan to cell walls and membranes of tobacco cells was directly observed by LSCM. Binding antagonist assays showed that unlabeled oligsaccharides apart from oligochitosn did not inhibit the observed binding of labeled oligochitosan to cell walls and membranes.
关键词[WOS]: HIGH-AFFINITY BINDING ;  N-ACETYLCHITOOLIGOSACCHARIDE ELICITOR ;  CULTURED RICE CELLS ;  DISEASE-RESISTANCE ;  DEFENSE RESPONSES ;  PLASMA-MEMBRANE ;  SALICYLIC-ACID ;  PLANT DEFENSE ;  CHITOSAN ;  INFECTION
语种: 英语
WOS记录号: WOS:000245260200006
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/140436
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Chinese Acad Sci, Dalian Inst Chem Phys, Dalian 116023, Peoples R China
2.Chinese Acad Sci, Grad Sch, Beijing 100049, Peoples R China
3.Shaanxi Normal Univ, Coll Life Sci, Xian 710062, Peoples R China

Recommended Citation:
Zhao, X. M.,She, X. P.,Yu, W.,et al. Effects of oligochitosans on tobacco cells and role of endogenous nitric oxide burst in the resistance of tobacco to Tobacco mosaic virus[J]. JOURNAL OF PLANT PATHOLOGY,2007,89(1):55-65.
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