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题名: Self-assembled epoxy-modified polymer coating on a poly(dimethylsiloxane) microchip for EOF inhibition and biopolymers separation
作者: Wu, Dapeng;  Qin, Jianhua;  Lin, Bingcheng
刊名: LAB ON A CHIP
发表日期: 2007
DOI: 10.1039/b708877a
卷: 7, 期:11, 页:1490-1496
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology ;  Life Sciences & Biomedicine ;  Physical Sciences
类目[WOS]: Biochemical Research Methods ;  Chemistry, Multidisciplinary ;  Nanoscience & Nanotechnology
研究领域[WOS]: Biochemistry & Molecular Biology ;  Chemistry ;  Science & Technology - Other Topics
英文摘要: A straightforward approach to generate a stable and protein- resistant poly(dimethylsiloxane) (PDMS) surface using self- assembled hydrophilic polymers is demonstrated in this work. Epoxy-modified polymers were directly adsorbed from aqueous solution onto plasma oxidized PDMS based on H-bond interaction, and epoxies of polymer and silanols on oxidized PDMS surface were crosslinked by heating at 110 degrees C. The coating process could be completed within half hour. Poly(dimethylacrylamide-co-glycidyl methacrylate) (PDMA-co-GMA), poly(vinyl pyrrolidone)g-glycidyl methacrylate (PVP-g-GMA) and poly(vinyl alcohol)-g-glycidyl methacrylate (PVAgGMA) (D. P. Wu, B. X. Zhao, Z. P. Dai, J. H. Qin and B. C. Lin, Lab Chip, 2006, 6, 942) were employed as examples here. Unlike PDMA, PVP, and PVA themselves, these epoxy-modified hydrophilic polymers could be directly used as static surface coatings on oxidized PDMS, and inhibited electroosmotic flow (EOF) within pH 3 - 11. It was also found that hard baking of PDMS at 150 degrees C for 24 hours before surface coating could greatly retard surface hydrophobicity recovery after oxygen plasma exposure, which strengthened epoxy-modified polymer coatings on oxidized PDMS surface, and resulted in EOF less than 0.2 x 10(-4) cm(2) V-1 s(-1) (pH 9.0) within two weeks. On epoxy-modified polymer coated PDMS microchips, basic proteins, peptides and DNA fragments could be separated satisfactorily, in which more than 2610 4 plates per 2 cm and less than 3% RSD (>8 runs) for migration time were obtained for lysozyme.
关键词[WOS]: NONSPECIFIC PROTEIN ADSORPTION ;  CAPILLARY-ZONE-ELECTROPHORESIS ;  FUSED-SILICA CAPILLARIES ;  LARGE-SCALE INTEGRATION ;  MICROFLUIDIC DEVICES ;  SURFACE MODIFICATION ;  GRAFT-POLYMERIZATION ;  MASS-SPECTROMETRY ;  PDMS SURFACE ;  CHIPS
语种: 英语
WOS记录号: WOS:000250428200022
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/140437
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Dalian Inst Chem Phys, Dalian 116023, Peoples R China
2.Chinese Acad Sci, Grad Sch, Dalian, Peoples R China

Recommended Citation:
Wu, Dapeng,Qin, Jianhua,Lin, Bingcheng. Self-assembled epoxy-modified polymer coating on a poly(dimethylsiloxane) microchip for EOF inhibition and biopolymers separation[J]. LAB ON A CHIP,2007,7(11):1490-1496.
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