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题名: Control of electroosmotic flow by a cation additive to enhance the separation of amino acids by micellar electrokinetic chromatography
作者: Qu, Qishu;  Tang, Xiaoqing;  Mangelings, Debby;  Wang, Chengyin;  Yang, Gongjun;  Hu, Xiaoya;  Yan, Chao
关键词: micellar electrokinetic chromatography ;  amino acids ;  electroosmotic flow ;  cation additive
刊名: JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
发表日期: 2007-06-15
DOI: 10.1016/j.jchromb.2007.02.061
卷: 853, 期:1-2, 页:31-37
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology ;  Life Sciences & Biomedicine ;  Physical Sciences
类目[WOS]: Biochemical Research Methods ;  Chemistry, Analytical
研究领域[WOS]: Biochemistry & Molecular Biology ;  Chemistry
英文摘要: The effect of a divalent cation (Mg2+) and 3 monovalent cations (Na+, Li+, and K+) as buffer additives on the electroosmotic flow (EOF) was investigated in order to improve the separation performance of p-acetamidobenzenesulfonyl fluoride (PAABS-F) derivatives of 20 standard amino acids by micellar electrokinetic chromatography (MEKC). The EOF can be decreased with increasing concentration of cations with the order of cations as Mg2+ > K+ > Na+ > Li+. However, it was found that the resolution cannot be improved easily using a buffer cation which is more capable of decreasing the EOF. Taking the migration time, resolution, and peak shape into account, Na+ is the best buffer additive, although the EOF decreased most using Mg2+. Using 20 mmol/L borate at pH 9.3 containing 140 mmol/L SDS and 20 mmol/L Na+ as electrolyte, 20 standard amino acids were successfully separated within 14 min. (c) 2007 Elsevier B.V. All rights reserved.
关键词[WOS]: CAPILLARY-ZONE-ELECTROPHORESIS ;  INDUCED FLUORESCENCE DETECTION ;  PRECOLUMN DERIVATIZATION ;  LIQUID-CHROMATOGRAPHY ;  ALIPHATIC-AMINES ;  BUFFER CATION ;  PROTEINS ;  REAGENT ;  PHENYLISOTHIOCYANATE ;  DERIVATIVES
语种: 英语
WOS记录号: WOS:000247704200004
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/140472
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Yangzhou Univ, Coll Chem & Chem Engn, Jiangsu Key Lab Environm Mat & Environm Engn, Yangzhou 250002, Peoples R China
2.Chinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatog Ctr, Dalian 116023, Peoples R China
3.Free Univ Brussels, Dept Analyt Chem & Pharmaceut Technol, B-1090 Brussels, Belgium

Recommended Citation:
Qu, Qishu,Tang, Xiaoqing,Mangelings, Debby,et al. Control of electroosmotic flow by a cation additive to enhance the separation of amino acids by micellar electrokinetic chromatography[J]. JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES,2007,853(1-2):31-37.
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