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题名: Rapid and simultaneous multiple genotyping of human Y chromosome from a human blood sample by on-chip enzymatic digestion and microchannel array electrophoresis coupled with blood sample pretreatment, and microscale PCR
作者: Zhang, Lihua;  Shinka, Toshikatsu;  Nakahori, Yutaka;  Kaji, Noritada;  Tokeshi, Manabu;  Baba, Yoshinobu
关键词: gene analysis ;  microchip ;  haplotyping ;  human Y chromosome
刊名: SENSORS AND ACTUATORS B-CHEMICAL
发表日期: 2007-01-30
DOI: 10.1016/j.snb.2006.09.020
卷: 121, 期:1, 页:124-128
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology ;  Physical Sciences ;  Technology
类目[WOS]: Chemistry, Analytical ;  Electrochemistry ;  Instruments & Instrumentation
研究领域[WOS]: Chemistry ;  Electrochemistry ;  Instruments & Instrumentation
英文摘要: In this paper, with distinct six DNA markers, 47z, SRY, YAP, IM, TB4Y and UTY, as the judgement standard, a combined technique for the rapid haplotyping (multiple genotyping) of human Y chromosome was developed by a DNA analysis system. By Generation capture disk, 5 min were enough to extract and purify DNA from five samples with only 4.5 L blood for each one. Consequently, LightCycler was chosen for the amplification of the six fragments of each sample. Under the optimized conditions, the simutaneous amplification of DNA extracted from five samples could be finished within 25 min. Among the six genomic fragments of each sample, the polymorphisms of YAP and IM could be distinguished directly from PCR products. The other four were single nucleotide polymorphisms. Therefore, microchip-based restriction fragment length polymorphism analysis was carried out. With a 12-channel microchip, in 8 min, two blood samples could be analyzed at the same time through on-chip digestion and the subsequent separation of DNA fragments in the channel. In summary, with the above-mentioned technique, microscale haplotyping of human Y chromosome could be finished at the average speed of 10 min/sample with only 4.5 mu L blood. (c) 2006 Elsevier B.V. All rights reserved.
关键词[WOS]: HAPLOTYPES ;  AMPLIFICATION ;  DEVICE ;  POPULATION
语种: 英语
WOS记录号: WOS:000244326400014
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/140492
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Nagoya Univ, Dept Appl Chem, Chikusa Ku, Grad Sch Engn, Nagoya, Aichi 4648603, Japan
2.Chinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatog R&A Ctr, Dalian 116023, Peoples R China
3.Univ Tokushima, CREST, Japan Sci & Technol Corp JST, Grad Sch Pharmaceut Sci,Dept Mol & Pharmaceut Bio, Tokushima 7708505, Japan
4.Furuno Elect Co LTD, Nishinomiya, Hyogo 6628580, Japan
5.Univ Tokushima, Sch Med, Dept Publ Hlth, Tokushima 7708505, Japan
6.Natl Inst Adv Ind Sci & Technol, Hlth Technol Res Ctr, Takamatsu, Kagawa 7610395, Japan

Recommended Citation:
Zhang, Lihua,Shinka, Toshikatsu,Nakahori, Yutaka,et al. Rapid and simultaneous multiple genotyping of human Y chromosome from a human blood sample by on-chip enzymatic digestion and microchannel array electrophoresis coupled with blood sample pretreatment, and microscale PCR[J]. SENSORS AND ACTUATORS B-CHEMICAL,2007,121(1):124-128.
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