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题名: Comparison of spiculogenesis in in vitro ADCP-primmorph and explants culture of marine sponge Hymeniacidon perleve with 3-TMOSPU supplementation
作者: Zhang, Wei;  Cao, Xupeng;  Zhang, Wei
刊名: JOURNAL OF BIOTECHNOLOGY
发表日期: 2007-09-01
DOI: 10.1016/j.jbiotec.2007.07.944
卷: 131, 期:2, 页:S31-S31
收录类别: ISTP ;  SCI
文章类型: Meeting Abstract
WOS标题词: Science & Technology ;  Life Sciences & Biomedicine
类目[WOS]: Biotechnology & Applied Microbiology
研究领域[WOS]: Biotechnology & Applied Microbiology
英文摘要: This study aims to test the feasibility of introducing functional chemical groups into biogenic silica spicules by examining the effect of supplementing a silican coupler [3-( trimethoxysilyl)propyl] urea ( 3-TMOSPU) as silica source in the cultures of archaeocytes-dominant-cell-population (ADCP) primmorphs and explants of the marine sponge Hymeniacidon perleve. Analysis by Fourier Transform Infrared Spectroscopy ( FT-IR) confirmed that the organic group in 3-TMOSPU was introduced into silica spicules. By comparing ADCP-primmorph cultures when supplemented with Na2SiO3, 3-TMOSPU supplementation showed no notable effect on the primmorphs development and cell locomotion behaviors. A decline in silicatein expression quantified by real-time RT-PCR was, however, observed during spiculogenesis. The decline was slower for the 3-TMOSPU group whereas significantly fewer spicules were formed. When sponge papillae explants were cultured, 3-TMOSPU supplementation had no negative effect on sponge growth but inhibited the growth biofouling of the diatom Nitzschia closterium. By monitoring the detectable Si concentration, it seemed that 3-TMOSPU was converted by the sponge and its conversion was related to spiculogenesis. Analysis of spicule dimensional changes indicated that the inhibition of spiculogenesis by 3- TMOSPU supplementation was less in ADCP-primmorphs culture due to lower 3-TMOSPU/detectable Si ratio in the media.
关键词[WOS]: CORAL-REEF SPONGES ;  SUBERITES-DOMUNCULA ;  SILICATEIN-ALPHA ;  HALICHONDRIA-PANICEA ;  ENZYMATIC-SYNTHESIS ;  CELL-PROLIFERATION ;  CATALYTIC-ACTIVITY ;  LOW-TEMPERATURE ;  GROWTH-RATES ;  NEUTRAL PH
语种: 英语
WOS记录号: WOS:000249687100048
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/140494
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Chinese Acad Sci, Dalain Inst Chem Phys, Marine Bioprod Engn Grp, Dalian, Liaoning, Peoples R China
2.Flinders Univ S Australia, Sch Med, Dept Med Biotechnol, Mol Bioproc & Bioprod Lab, Adelaide, SA 5001, Australia

Recommended Citation:
Zhang, Wei,Cao, Xupeng,Zhang, Wei. Comparison of spiculogenesis in in vitro ADCP-primmorph and explants culture of marine sponge Hymeniacidon perleve with 3-TMOSPU supplementation[J]. JOURNAL OF BIOTECHNOLOGY,2007,131(2):S31-S31.
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