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Comparison of spiculogenesis in in vitro ADCP-primmorph and explants culture of marine sponge Hymeniacidon perleve with 3-TMOSPU supplementation
Zhang, Wei; Cao, Xupeng; Zhang, Wei
Source PublicationJOURNAL OF BIOTECHNOLOGY
2007-09-01
DOI10.1016/j.jbiotec.2007.07.944
Volume131Issue:2Pages:S31-S31
Indexed ByISTP ; SCI
SubtypeMeeting Abstract
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
WOS SubjectBiotechnology & Applied Microbiology
WOS Research AreaBiotechnology & Applied Microbiology
WOS KeywordCORAL-REEF SPONGES ; SUBERITES-DOMUNCULA ; SILICATEIN-ALPHA ; HALICHONDRIA-PANICEA ; ENZYMATIC-SYNTHESIS ; CELL-PROLIFERATION ; CATALYTIC-ACTIVITY ; LOW-TEMPERATURE ; GROWTH-RATES ; NEUTRAL PH
AbstractThis study aims to test the feasibility of introducing functional chemical groups into biogenic silica spicules by examining the effect of supplementing a silican coupler [3-( trimethoxysilyl)propyl] urea ( 3-TMOSPU) as silica source in the cultures of archaeocytes-dominant-cell-population (ADCP) primmorphs and explants of the marine sponge Hymeniacidon perleve. Analysis by Fourier Transform Infrared Spectroscopy ( FT-IR) confirmed that the organic group in 3-TMOSPU was introduced into silica spicules. By comparing ADCP-primmorph cultures when supplemented with Na2SiO3, 3-TMOSPU supplementation showed no notable effect on the primmorphs development and cell locomotion behaviors. A decline in silicatein expression quantified by real-time RT-PCR was, however, observed during spiculogenesis. The decline was slower for the 3-TMOSPU group whereas significantly fewer spicules were formed. When sponge papillae explants were cultured, 3-TMOSPU supplementation had no negative effect on sponge growth but inhibited the growth biofouling of the diatom Nitzschia closterium. By monitoring the detectable Si concentration, it seemed that 3-TMOSPU was converted by the sponge and its conversion was related to spiculogenesis. Analysis of spicule dimensional changes indicated that the inhibition of spiculogenesis by 3- TMOSPU supplementation was less in ADCP-primmorphs culture due to lower 3-TMOSPU/detectable Si ratio in the media.
Language英语
WOS IDWOS:000249687100048
Citation statistics
Document Type期刊论文
Identifierhttp://cas-ir.dicp.ac.cn/handle/321008/140494
Collection中国科学院大连化学物理研究所
Affiliation1.Chinese Acad Sci, Dalain Inst Chem Phys, Marine Bioprod Engn Grp, Dalian, Liaoning, Peoples R China
2.Flinders Univ S Australia, Sch Med, Dept Med Biotechnol, Mol Bioproc & Bioprod Lab, Adelaide, SA 5001, Australia
Recommended Citation
GB/T 7714
Zhang, Wei,Cao, Xupeng,Zhang, Wei. Comparison of spiculogenesis in in vitro ADCP-primmorph and explants culture of marine sponge Hymeniacidon perleve with 3-TMOSPU supplementation[J]. JOURNAL OF BIOTECHNOLOGY,2007,131(2):S31-S31.
APA Zhang, Wei,Cao, Xupeng,&Zhang, Wei.(2007).Comparison of spiculogenesis in in vitro ADCP-primmorph and explants culture of marine sponge Hymeniacidon perleve with 3-TMOSPU supplementation.JOURNAL OF BIOTECHNOLOGY,131(2),S31-S31.
MLA Zhang, Wei,et al."Comparison of spiculogenesis in in vitro ADCP-primmorph and explants culture of marine sponge Hymeniacidon perleve with 3-TMOSPU supplementation".JOURNAL OF BIOTECHNOLOGY 131.2(2007):S31-S31.
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