中国科学院大连化学物理研究所机构知识库
Advanced  
DICP OpenIR  > 中国科学院大连化学物理研究所  > 期刊论文
题名: Ouantification of cis-abienol in oriental tobacco leaves by LC
作者: Ding, Li;  Xie, Fuwei;  Zhao, Mingyue;  Xie, Jianping;  Xu, Guowang
关键词: liquid chromatography ;  cis-Abienol ;  oriental tobacco
刊名: CHROMATOGRAPHIA
发表日期: 2007-10-01
DOI: 10.1365/s10337-007-0365-9
卷: 66, 期:7-8, 页:529-532
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology ;  Life Sciences & Biomedicine ;  Physical Sciences
类目[WOS]: Biochemical Research Methods ;  Chemistry, Analytical
研究领域[WOS]: Biochemistry & Molecular Biology ;  Chemistry
英文摘要: A rapid and accurate method for the quantification of cis-abienol in oriental tobacco leaves by normal phase liquid chromatography was developed. Freeze-dried tobacco samples were sonicated in methylene chloride for 10 min. The supernatant was purified using a silica gel solid phase extraction cartridge. Ten milliliter of the resulting methylene chloride eluate was collected, then separated on a 250 x 4.6 mm, 5 pm particle-size CN column with n-hexane: ethyl acetate, 100:2 (v/v) at a flow rate of 1 mL min(-1). cis-Abienol was detected by UV absorption at 254 nm. The linear range was from 2.14 x 10(-4) to 4.28 x 10(-2) mg mL(-1) and the correlation coefficient was 1.000. The average recovery was 98.7, 105.2 and 103.1% in five replicated sets of tobacco samples spiked with 0.2856, 0.7140 and 1.904 mg cis-abienol. The relative standard deviations (RSDs) were 1.04, 0.63 and 1.25%, respectively (n = 5). Limit of detection (S/N = 3) was 21.84 pg g(-1) and limit of quantification (S/N = 10) was 72.80 mu g g(-1). The method was found to be suitable for determination of cis-abienol in oriental tobacco leaves. Furthermore, pure cis-abienol used for method validation was obtained by preparative reversed phase high -performance liquid chromatography. Identification was performed by UV detection, nuclear magnetic resonance and mass spectrometry.
关键词[WOS]: CHEMISTRY ;  LEAF
语种: 英语
WOS记录号: WOS:000250366300012
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/140500
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

Files in This Item:

There are no files associated with this item.


作者单位: 1.CNTC, Zhengzhou Tobacco Res Inst, Dept Chem, Zhengzhou 450001, Peoples R China
2.Chinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatog R&A Ctr, Dalian 116023, Peoples R China

Recommended Citation:
Ding, Li,Xie, Fuwei,Zhao, Mingyue,et al. Ouantification of cis-abienol in oriental tobacco leaves by LC[J]. CHROMATOGRAPHIA,2007,66(7-8):529-532.
Service
 Recommend this item
 Sava as my favorate item
 Show this item's statistics
 Export Endnote File
Google Scholar
 Similar articles in Google Scholar
 [Ding, Li]'s Articles
 [Xie, Fuwei]'s Articles
 [Zhao, Mingyue]'s Articles
CSDL cross search
 Similar articles in CSDL Cross Search
 [Ding, Li]‘s Articles
 [Xie, Fuwei]‘s Articles
 [Zhao, Mingyue]‘s Articles
Related Copyright Policies
Null
Social Bookmarking
  Add to CiteULike  Add to Connotea  Add to Del.icio.us  Add to Digg  Add to Reddit 
所有评论 (0)
暂无评论
 
评注功能仅针对注册用户开放,请您登录
您对该条目有什么异议,请填写以下表单,管理员会尽快联系您。
内 容:
Email:  *
单位:
验证码:   刷新
您在IR的使用过程中有什么好的想法或者建议可以反馈给我们。
标 题:
 *
内 容:
Email:  *
验证码:   刷新

Items in IR are protected by copyright, with all rights reserved, unless otherwise indicated.

 

 

Valid XHTML 1.0!
Powered by CSpace