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题名: Cooperative effects in the fluorescent labeling of proteins studied by high-performance liquid chromatography
作者: Qiao Xiao-Qiang;  Zhang Lin;  Liang Zhen;  Zhang Wei-Bing;  Zhang Li-Hua;  Zhang Yu-Kui
关键词: fluorescent labeling ;  protein ;  cooperative effect ;  high-performance liquid chromatography (HPLC)
刊名: CHEMICAL JOURNAL OF CHINESE UNIVERSITIES-CHINESE
发表日期: 2007-09-01
卷: 28, 期:9, 页:1657-1659
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology ;  Physical Sciences
类目[WOS]: Chemistry, Multidisciplinary
研究领域[WOS]: Chemistry
英文摘要: To improve the detection sensitivity of low abundance proteins in samples, fluorescence labeling might be a good solution method. In this manuscript, with 5-( {2-[ (iodoacetyl) amino] ethyl} amino) naphthalene-1-sulfonic acid (1,5-I-AEDANS) as the derivatizing reagent, the cooperative effects of insulin and BSA during the derivatization were studied. Through HPLC analysis, it was found that with,BSA added, the detection sensitivity of the derivatized insulin could be improved. When the concentration ratio of BSA to insulin reached 200: 1, the peak area of insulin was increased by over 4-fold, which demonstrates that the existence of BSA had a positive effect on the derivatization of insulin. Furthermore, even when the ratio reached 1000: 1, the SIN of insulin was 8, which could not be detected without BSA being added. However, on the contrary, with insulin being added, the peak area of the derivatized BSA was decreased, demonstrating that insulin had a negative effect on that of BSA. All these results might be of great significance to the detection of low abundance proteins in proteome study.
关键词[WOS]: MOLECULES ;  PEPTIDES ;  BINDING
语种: 英语
WOS记录号: WOS:000249629600013
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/140501
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Chinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatog R&A Ctr, Dalian 116023, Peoples R China

Recommended Citation:
Qiao Xiao-Qiang,Zhang Lin,Liang Zhen,et al. Cooperative effects in the fluorescent labeling of proteins studied by high-performance liquid chromatography[J]. CHEMICAL JOURNAL OF CHINESE UNIVERSITIES-CHINESE,2007,28(9):1657-1659.
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