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题名: Spectroscopy study on the interaction of colchicine and human serum albumin
作者: Ma Jun-Yan2;  Chen Ke-Hai2;  Zheng Xue-Fang1, 2;  Guo Ming1;  Ma Jing2;  Tang Qian2;  Wang Yu-Lian2;  Hu Jie-Han1, 3
关键词: colchicine ;  human serum albumin ;  UV-Vis spectra ;  fluorescence spectra ;  circular dichroism spectra
刊名: SPECTROSCOPY AND SPECTRAL ANALYSIS
发表日期: 2007-12-01
卷: 27, 期:12, 页:2485-2489
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology ;  Technology
类目[WOS]: Spectroscopy
研究领域[WOS]: Spectroscopy
英文摘要: The binding reaction of colchicine with human serum albumin(HSA) was studied by UV-Vis absorption, fluorescence and circular dichroism. spectrometry. The results indicated that colchicine led to the increase in UV absorption and the quenching of intrinsic fluorescence of HSA. As the temperature increased, the quenching constant K-sv decreased. The binding constants and the numbers of the binding sites of the interaction between colchicine and HSA at different temperatures were obtained. The thermodynamic parameters, enthalpy change (Delta H) and entropy change (Delta S), were calculated to be - 11. 66 kJ . mol(-1) and 51. 507 J (mol . K)(-1) respectively according to Van't Hoff equation, which suggested that the main binding force between colchicine and HSA was static interaction. The protein conformation was altered(CD date) with decreasing of a-helices in the presence of colchicine. The results showed that the quenching mechanism of the combination of colchicine with human serum albumin was a static quenching procedure.
关键词[WOS]: FLUORESCENCE ;  MECHANISM ;  BINDING
语种: 英语
WOS记录号: WOS:000253064500026
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/140519
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Dalian Univ, Liaoning Key Lab Bioorgan Chem, Dalian 116622, Peoples R China
2.Dalian Univ, Coll Bioengn, Dalian 116622, Peoples R China
3.Chinese Acad Sci, Dalian Inst Chem Phys, Dalian 116023, Peoples R China

Recommended Citation:
Ma Jun-Yan,Chen Ke-Hai,Zheng Xue-Fang,et al. Spectroscopy study on the interaction of colchicine and human serum albumin[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS,2007,27(12):2485-2489.
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