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Spectroscopy study on the interaction of colchicine and human serum albumin
Ma Jun-Yan2; Chen Ke-Hai2; Zheng Xue-Fang1,2; Guo Ming1; Ma Jing2; Tang Qian2; Wang Yu-Lian2; Hu Jie-Han1,3
KeywordColchicine Human Serum Albumin Uv-vis Spectra Fluorescence Spectra Circular Dichroism Spectra
Source PublicationSPECTROSCOPY AND SPECTRAL ANALYSIS
2007-12-01
Volume27Issue:12Pages:2485-2489
Indexed BySCI
SubtypeArticle
WOS HeadingsScience & Technology ; Technology
WOS SubjectSpectroscopy
WOS Research AreaSpectroscopy
WOS KeywordFLUORESCENCE ; MECHANISM ; BINDING
AbstractThe binding reaction of colchicine with human serum albumin(HSA) was studied by UV-Vis absorption, fluorescence and circular dichroism. spectrometry. The results indicated that colchicine led to the increase in UV absorption and the quenching of intrinsic fluorescence of HSA. As the temperature increased, the quenching constant K-sv decreased. The binding constants and the numbers of the binding sites of the interaction between colchicine and HSA at different temperatures were obtained. The thermodynamic parameters, enthalpy change (Delta H) and entropy change (Delta S), were calculated to be - 11. 66 kJ . mol(-1) and 51. 507 J (mol . K)(-1) respectively according to Van't Hoff equation, which suggested that the main binding force between colchicine and HSA was static interaction. The protein conformation was altered(CD date) with decreasing of a-helices in the presence of colchicine. The results showed that the quenching mechanism of the combination of colchicine with human serum albumin was a static quenching procedure.
Language英语
WOS IDWOS:000253064500026
Citation statistics
Cited Times:5[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://cas-ir.dicp.ac.cn/handle/321008/140519
Collection中国科学院大连化学物理研究所
Affiliation1.Dalian Univ, Liaoning Key Lab Bioorgan Chem, Dalian 116622, Peoples R China
2.Dalian Univ, Coll Bioengn, Dalian 116622, Peoples R China
3.Chinese Acad Sci, Dalian Inst Chem Phys, Dalian 116023, Peoples R China
Recommended Citation
GB/T 7714
Ma Jun-Yan,Chen Ke-Hai,Zheng Xue-Fang,et al. Spectroscopy study on the interaction of colchicine and human serum albumin[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS,2007,27(12):2485-2489.
APA Ma Jun-Yan.,Chen Ke-Hai.,Zheng Xue-Fang.,Guo Ming.,Ma Jing.,...&Hu Jie-Han.(2007).Spectroscopy study on the interaction of colchicine and human serum albumin.SPECTROSCOPY AND SPECTRAL ANALYSIS,27(12),2485-2489.
MLA Ma Jun-Yan,et al."Spectroscopy study on the interaction of colchicine and human serum albumin".SPECTROSCOPY AND SPECTRAL ANALYSIS 27.12(2007):2485-2489.
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