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Direct electrochemistry and electrocatalysis of cytochrome c immobilized on gold nanoparticles-chitosan-carbon nanotubes-modified electrode
Xiang, Cuili1,2; Zou, Yongjin1,2; Sun, Li-Xian1; Xu, Fen1
KeywordCytochrome c Gold Nanoparticles Multi-walled Carbon Nanotubes Chitosan Nanohybrid Direct Electron Transfer
Source PublicationTALANTA
2007-11-30
DOI10.1016/j.talanta.2007.05.050
Volume74Issue:2Pages:206-211
Indexed BySCI
SubtypeArticle
WOS HeadingsScience & Technology ; Physical Sciences
WOS SubjectChemistry, Analytical
WOS Research AreaChemistry
WOS KeywordHYDROGEN-PEROXIDE ; HYBRID COMPOSITES ; BIOSENSOR ; FILM ; SENSOR ; INTERFACE ; OXIDATION ; PROTEINS ; SURFACES ; BEHAVIOR
AbstractA robust and effective nanohybrid film based on gold nanoparticles (GNPs)/chitosan (Chit)/multi-walled carbon nanotubes (MWNTs) was prepared by a layer-by-layer self-assembly technique. Cytochrome c (Cyt c) was successfully immobilized on the nanohybrid film modified glassy carbon (GC) electrode by cyclic voltammetry. The direct electron transfer between Cyt c and the modified electrode was investigated in detail. Cyt c shows a couple of quasi-reversible and well-defined cyclic voltammetry peaks with a formal potential (E-0) of -0. 16 V (versus Ag/AgCl) in pH 7.0 phosphate buffer solution (PBS). The Cyt c/GNPs/Chit/MWNTs modified GC electrode gives an improved electrocatalytic activity towards the reduction of hydrogen peroxide (H2O2). The sensitivity is 92.21 mu A mM(-1) cm(-2) and the calculated apparent Michael is-Menten constant (K-m(app)) is 0.791 mM, indicating a high-catalytic activity of Cyt c. The catalysis currents increase linearly to the H2O2 concentration in a wide range of 1.5 x 10(-6) to 5. 1 x 10(-4) M with a correlation coefficient 0.999. The detection limit is 9.0 x 10(-7) M (at the ratio of signal to noise, S/N = 3). Moreover, the modified electrode displays rapid response (5 s) to H2O2, and possesses good stability and reproducibility. (c) 2007 Elsevier B.V All fights reserved.
Language英语
WOS IDWOS:000251629800006
Citation statistics
Cited Times:93[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://cas-ir.dicp.ac.cn/handle/321008/140521
Collection中国科学院大连化学物理研究所
Affiliation1.Chinese Acad Sci, Dalian Inst Chem Phys, Mat & Thermochem Lab, Dalian 116023, Peoples R China
2.Chinese Acad Sci, Grad Sch, Beijing 100049, Peoples R China
Recommended Citation
GB/T 7714
Xiang, Cuili,Zou, Yongjin,Sun, Li-Xian,et al. Direct electrochemistry and electrocatalysis of cytochrome c immobilized on gold nanoparticles-chitosan-carbon nanotubes-modified electrode[J]. TALANTA,2007,74(2):206-211.
APA Xiang, Cuili,Zou, Yongjin,Sun, Li-Xian,&Xu, Fen.(2007).Direct electrochemistry and electrocatalysis of cytochrome c immobilized on gold nanoparticles-chitosan-carbon nanotubes-modified electrode.TALANTA,74(2),206-211.
MLA Xiang, Cuili,et al."Direct electrochemistry and electrocatalysis of cytochrome c immobilized on gold nanoparticles-chitosan-carbon nanotubes-modified electrode".TALANTA 74.2(2007):206-211.
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