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题名: Direct electrochemistry and electrocatalysis of cytochrome c immobilized on gold nanoparticles-chitosan-carbon nanotubes-modified electrode
作者: Xiang, Cuili1, 2;  Zou, Yongjin1, 2;  Sun, Li-Xian1;  Xu, Fen1
关键词: cytochrome c ;  gold nanoparticles ;  multi-walled carbon nanotubes ;  chitosan ;  nanohybrid ;  direct electron transfer
刊名: TALANTA
发表日期: 2007-11-30
DOI: 10.1016/j.talanta.2007.05.050
卷: 74, 期:2, 页:206-211
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology ;  Physical Sciences
类目[WOS]: Chemistry, Analytical
研究领域[WOS]: Chemistry
英文摘要: A robust and effective nanohybrid film based on gold nanoparticles (GNPs)/chitosan (Chit)/multi-walled carbon nanotubes (MWNTs) was prepared by a layer-by-layer self-assembly technique. Cytochrome c (Cyt c) was successfully immobilized on the nanohybrid film modified glassy carbon (GC) electrode by cyclic voltammetry. The direct electron transfer between Cyt c and the modified electrode was investigated in detail. Cyt c shows a couple of quasi-reversible and well-defined cyclic voltammetry peaks with a formal potential (E-0) of -0. 16 V (versus Ag/AgCl) in pH 7.0 phosphate buffer solution (PBS). The Cyt c/GNPs/Chit/MWNTs modified GC electrode gives an improved electrocatalytic activity towards the reduction of hydrogen peroxide (H2O2). The sensitivity is 92.21 mu A mM(-1) cm(-2) and the calculated apparent Michael is-Menten constant (K-m(app)) is 0.791 mM, indicating a high-catalytic activity of Cyt c. The catalysis currents increase linearly to the H2O2 concentration in a wide range of 1.5 x 10(-6) to 5. 1 x 10(-4) M with a correlation coefficient 0.999. The detection limit is 9.0 x 10(-7) M (at the ratio of signal to noise, S/N = 3). Moreover, the modified electrode displays rapid response (5 s) to H2O2, and possesses good stability and reproducibility. (c) 2007 Elsevier B.V All fights reserved.
关键词[WOS]: HYDROGEN-PEROXIDE ;  HYBRID COMPOSITES ;  BIOSENSOR ;  FILM ;  SENSOR ;  INTERFACE ;  OXIDATION ;  PROTEINS ;  SURFACES ;  BEHAVIOR
语种: 英语
WOS记录号: WOS:000251629800006
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/140521
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Chinese Acad Sci, Dalian Inst Chem Phys, Mat & Thermochem Lab, Dalian 116023, Peoples R China
2.Chinese Acad Sci, Grad Sch, Beijing 100049, Peoples R China

Recommended Citation:
Xiang, Cuili,Zou, Yongjin,Sun, Li-Xian,et al. Direct electrochemistry and electrocatalysis of cytochrome c immobilized on gold nanoparticles-chitosan-carbon nanotubes-modified electrode[J]. TALANTA,2007,74(2):206-211.
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