DICP OpenIR
Purification and in vitro cultivation of archaeocytes (stem cells) of the marine sponge Hymeniacidon perleve (Demospongiae)
Sun, Liming; Song, Yuefan; Qu, Yi; Yu, Xingju; Zhang, Wei
KeywordArchaeocyte Invertebrate Cell Culture Marine Sponge Porifera Hymeniacidon Perleve (Demospongiae)
Source PublicationCELL AND TISSUE RESEARCH
2007-04-01
DOI10.1007/s00441-006-0342-x
Volume328Issue:1Pages:223-237
Indexed BySCI
SubtypeArticle
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
WOS SubjectCell Biology
WOS Research AreaCell Biology
WOS KeywordDNA POLYMERASE-DELTA ; NUCLEAR-PROTEIN CYCLIN ; AUXILIARY PROTEIN ; SUBERITES-DOMUNCULA ; TELOMERASE ACTIVITY ; NATURAL-PRODUCTS ; CELLULAR-ORIGIN ; ANTIGEN ; CULTURES ; PRIMMORPHS
AbstractMarine sponges (Porifera) are the best source of marine bioactive metabolites for drug discovery and development, although the sustainable production of most sponge-derived metabolites remains a difficult task. In vitro cultivation of sponge cells in bioreactors has been proposed as a promising technology. However, no continuous cell line has as yet been developed. Archaeocytes are considered to be toti/multipotent stem cells in sponges and, when purified, may allow the development of continuous sponge cell lines. As a prerequisite, we have developed a novel four-step protocol for the purification of archaeocytes from a marine sponge, Hymeniacidon perleve: (1) differential centrifugation to separate large sponge cells including archaeocytes; (2) selective agglomeration in low-Ca2+/Mg2+ artificial seawater in which living archaeocytes form small loose aggregates with some pinacocytes and collencytes; (3) differential adherence to remove anchorage-dependent pinacocytes, collencytes and other mesohyl cells; (4) Ficoll-Vrografin density gradient centrifugation to purify archaeocytes. The final purity of archaeocytes is greater than 80%. The proliferation potential of the archaeocytes has been demonstrated by high levels of BrdU incorporation, PCNA expression and telomerase activity. In 4-day primary cultures, the purified archaeocytes show a 2.5-fold increase in total cell number. This study opens an important avenue towards developing sponge cell cultures for the commercial exploitation of sponge- derived drugs.
Language英语
WOS IDWOS:000244888100020
Citation statistics
Cited Times:23[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://cas-ir.dicp.ac.cn/handle/321008/140524
Collection中国科学院大连化学物理研究所
Affiliation1.Chinese Acad Sci, Dalian Inst Chem Phys, Marine Bioprod Engn Grp, Dalian 116023, Peoples R China
2.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China
3.Flinders Univ S Australia, Sch Med, Dept Med Biotechnol, Bedford Pk, SA 5042, Australia
Recommended Citation
GB/T 7714
Sun, Liming,Song, Yuefan,Qu, Yi,et al. Purification and in vitro cultivation of archaeocytes (stem cells) of the marine sponge Hymeniacidon perleve (Demospongiae)[J]. CELL AND TISSUE RESEARCH,2007,328(1):223-237.
APA Sun, Liming,Song, Yuefan,Qu, Yi,Yu, Xingju,&Zhang, Wei.(2007).Purification and in vitro cultivation of archaeocytes (stem cells) of the marine sponge Hymeniacidon perleve (Demospongiae).CELL AND TISSUE RESEARCH,328(1),223-237.
MLA Sun, Liming,et al."Purification and in vitro cultivation of archaeocytes (stem cells) of the marine sponge Hymeniacidon perleve (Demospongiae)".CELL AND TISSUE RESEARCH 328.1(2007):223-237.
Files in This Item:
There are no files associated with this item.
Related Services
Recommend this item
Bookmark
Usage statistics
Export to Endnote
Google Scholar
Similar articles in Google Scholar
[Sun, Liming]'s Articles
[Song, Yuefan]'s Articles
[Qu, Yi]'s Articles
Baidu academic
Similar articles in Baidu academic
[Sun, Liming]'s Articles
[Song, Yuefan]'s Articles
[Qu, Yi]'s Articles
Bing Scholar
Similar articles in Bing Scholar
[Sun, Liming]'s Articles
[Song, Yuefan]'s Articles
[Qu, Yi]'s Articles
Terms of Use
No data!
Social Bookmark/Share
All comments (0)
No comment.
 

Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.