DICP OpenIR
Purification and in vitro cultivation of archaeocytes (stem cells) of the marine sponge Hymeniacidon perleve (Demospongiae)
Sun, Liming; Song, Yuefan; Qu, Yi; Yu, Xingju; Zhang, Wei
关键词Archaeocyte Invertebrate Cell Culture Marine Sponge Porifera Hymeniacidon Perleve (Demospongiae)
刊名CELL AND TISSUE RESEARCH
2007-04-01
DOI10.1007/s00441-006-0342-x
328期:1页:223-237
收录类别SCI
文章类型Article
WOS标题词Science & Technology ; Life Sciences & Biomedicine
类目[WOS]Cell Biology
研究领域[WOS]Cell Biology
关键词[WOS]DNA POLYMERASE-DELTA ; NUCLEAR-PROTEIN CYCLIN ; AUXILIARY PROTEIN ; SUBERITES-DOMUNCULA ; TELOMERASE ACTIVITY ; NATURAL-PRODUCTS ; CELLULAR-ORIGIN ; ANTIGEN ; CULTURES ; PRIMMORPHS
英文摘要Marine sponges (Porifera) are the best source of marine bioactive metabolites for drug discovery and development, although the sustainable production of most sponge-derived metabolites remains a difficult task. In vitro cultivation of sponge cells in bioreactors has been proposed as a promising technology. However, no continuous cell line has as yet been developed. Archaeocytes are considered to be toti/multipotent stem cells in sponges and, when purified, may allow the development of continuous sponge cell lines. As a prerequisite, we have developed a novel four-step protocol for the purification of archaeocytes from a marine sponge, Hymeniacidon perleve: (1) differential centrifugation to separate large sponge cells including archaeocytes; (2) selective agglomeration in low-Ca2+/Mg2+ artificial seawater in which living archaeocytes form small loose aggregates with some pinacocytes and collencytes; (3) differential adherence to remove anchorage-dependent pinacocytes, collencytes and other mesohyl cells; (4) Ficoll-Vrografin density gradient centrifugation to purify archaeocytes. The final purity of archaeocytes is greater than 80%. The proliferation potential of the archaeocytes has been demonstrated by high levels of BrdU incorporation, PCNA expression and telomerase activity. In 4-day primary cultures, the purified archaeocytes show a 2.5-fold increase in total cell number. This study opens an important avenue towards developing sponge cell cultures for the commercial exploitation of sponge- derived drugs.
语种英语
WOS记录号WOS:000244888100020
引用统计
被引频次:22[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://cas-ir.dicp.ac.cn/handle/321008/140524
专题中国科学院大连化学物理研究所
作者单位1.Chinese Acad Sci, Dalian Inst Chem Phys, Marine Bioprod Engn Grp, Dalian 116023, Peoples R China
2.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China
3.Flinders Univ S Australia, Sch Med, Dept Med Biotechnol, Bedford Pk, SA 5042, Australia
推荐引用方式
GB/T 7714
Sun, Liming,Song, Yuefan,Qu, Yi,et al. Purification and in vitro cultivation of archaeocytes (stem cells) of the marine sponge Hymeniacidon perleve (Demospongiae)[J]. CELL AND TISSUE RESEARCH,2007,328(1):223-237.
APA Sun, Liming,Song, Yuefan,Qu, Yi,Yu, Xingju,&Zhang, Wei.(2007).Purification and in vitro cultivation of archaeocytes (stem cells) of the marine sponge Hymeniacidon perleve (Demospongiae).CELL AND TISSUE RESEARCH,328(1),223-237.
MLA Sun, Liming,et al."Purification and in vitro cultivation of archaeocytes (stem cells) of the marine sponge Hymeniacidon perleve (Demospongiae)".CELL AND TISSUE RESEARCH 328.1(2007):223-237.
条目包含的文件
条目无相关文件。
个性服务
推荐该条目
保存到收藏夹
查看访问统计
导出为Endnote文件
谷歌学术
谷歌学术中相似的文章
[Sun, Liming]的文章
[Song, Yuefan]的文章
[Qu, Yi]的文章
百度学术
百度学术中相似的文章
[Sun, Liming]的文章
[Song, Yuefan]的文章
[Qu, Yi]的文章
必应学术
必应学术中相似的文章
[Sun, Liming]的文章
[Song, Yuefan]的文章
[Qu, Yi]的文章
相关权益政策
暂无数据
收藏/分享
所有评论 (0)
暂无评论
 

除非特别说明,本系统中所有内容都受版权保护,并保留所有权利。