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题名: Purification and in vitro cultivation of archaeocytes (stem cells) of the marine sponge Hymeniacidon perleve (Demospongiae)
作者: Sun, Liming;  Song, Yuefan;  Qu, Yi;  Yu, Xingju;  Zhang, Wei
关键词: archaeocyte ;  invertebrate cell culture ;  marine sponge ;  Porifera ;  Hymeniacidon perleve (Demospongiae)
刊名: CELL AND TISSUE RESEARCH
发表日期: 2007-04-01
DOI: 10.1007/s00441-006-0342-x
卷: 328, 期:1, 页:223-237
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology ;  Life Sciences & Biomedicine
类目[WOS]: Cell Biology
研究领域[WOS]: Cell Biology
英文摘要: Marine sponges (Porifera) are the best source of marine bioactive metabolites for drug discovery and development, although the sustainable production of most sponge-derived metabolites remains a difficult task. In vitro cultivation of sponge cells in bioreactors has been proposed as a promising technology. However, no continuous cell line has as yet been developed. Archaeocytes are considered to be toti/multipotent stem cells in sponges and, when purified, may allow the development of continuous sponge cell lines. As a prerequisite, we have developed a novel four-step protocol for the purification of archaeocytes from a marine sponge, Hymeniacidon perleve: (1) differential centrifugation to separate large sponge cells including archaeocytes; (2) selective agglomeration in low-Ca2+/Mg2+ artificial seawater in which living archaeocytes form small loose aggregates with some pinacocytes and collencytes; (3) differential adherence to remove anchorage-dependent pinacocytes, collencytes and other mesohyl cells; (4) Ficoll-Vrografin density gradient centrifugation to purify archaeocytes. The final purity of archaeocytes is greater than 80%. The proliferation potential of the archaeocytes has been demonstrated by high levels of BrdU incorporation, PCNA expression and telomerase activity. In 4-day primary cultures, the purified archaeocytes show a 2.5-fold increase in total cell number. This study opens an important avenue towards developing sponge cell cultures for the commercial exploitation of sponge- derived drugs.
关键词[WOS]: DNA POLYMERASE-DELTA ;  NUCLEAR-PROTEIN CYCLIN ;  AUXILIARY PROTEIN ;  SUBERITES-DOMUNCULA ;  TELOMERASE ACTIVITY ;  NATURAL-PRODUCTS ;  CELLULAR-ORIGIN ;  ANTIGEN ;  CULTURES ;  PRIMMORPHS
语种: 英语
WOS记录号: WOS:000244888100020
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/140524
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Chinese Acad Sci, Dalian Inst Chem Phys, Marine Bioprod Engn Grp, Dalian 116023, Peoples R China
2.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China
3.Flinders Univ S Australia, Sch Med, Dept Med Biotechnol, Bedford Pk, SA 5042, Australia

Recommended Citation:
Sun, Liming,Song, Yuefan,Qu, Yi,et al. Purification and in vitro cultivation of archaeocytes (stem cells) of the marine sponge Hymeniacidon perleve (Demospongiae)[J]. CELL AND TISSUE RESEARCH,2007,328(1):223-237.
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