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题名: Sample loops-valve interface of comprehensive two-dimensional liquid chromatography (NPLC x RPLC) and its application
作者: Tian Hong-Zhe;  Xu Jing;  Guan Ya-Feng
关键词: comprehensive two-dimensional LC ;  NPLC x RPLC coupling technique ;  sample loops-valve interface ;  traditional Chinese medicine ;  Radix salviae miltiorrhiza bage
刊名: CHEMICAL JOURNAL OF CHINESE UNIVERSITIES-CHINESE
发表日期: 2007-04-10
卷: 28, 期:4, 页:630-634
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology ;  Physical Sciences
类目[WOS]: Chemistry, Multidisciplinary
研究领域[WOS]: Chemistry
英文摘要: A comprehensive two-dimensional liquid chromatography( NPLC X RPLC) was developed. Normal phase liquid chromatography (NPLC) with a CN microcolumn was used as the first dimension, and reversed-phase liquid chromatography( RPLC) with a monolithic column was used as the second dimension. The interface was based on a ten-port, two-position valve with two storage loops. The first column was operated at a flow rate of 8 mu L/min in isocratic mode, while the monolithic column flow rate was 4 mL/min and was operated in gradient mode. The sample loops had a volume of 12 mu L each, and the analysis time in the second dimension. was 1.5 min. The primary fractions were stored into the two storage loops alternately, and then were separated by the secondary column sequentially. AUV detector was used after the secondary column. The use of a monolithic column in the second dimension permits a very fast analysis operating at highter flow rates without loss of resolution, since the monolithic column has higher permeability than conventional packed columns. The incompatibility of the two dimensional mobile phases was avoided because the flow rate of the first dimension is only 1/500 of that of the second dimension. The performance of the system was evaluated by several standards mixture and Radix salviae miltiorrhiza bage extract and the advantage of using 2D-LC over one-dimensional chromatographic techniques is clearly demonstrated in the analysis of the extract.
关键词[WOS]: RESOLUTION
语种: 英语
WOS记录号: WOS:000245874300007
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/140562
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Chinese Acad Sci, Dalian Inst Chem Phys, Dept Instrumentat & Analyt Chem, Dalian 116023, Peoples R China

Recommended Citation:
Tian Hong-Zhe,Xu Jing,Guan Ya-Feng. Sample loops-valve interface of comprehensive two-dimensional liquid chromatography (NPLC x RPLC) and its application[J]. CHEMICAL JOURNAL OF CHINESE UNIVERSITIES-CHINESE,2007,28(4):630-634.
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