DICP OpenIR
Screening and analysis of bioactive compounds in traditional Chinese medicines using cell extract and gas chromatography-mass spectrometry
Zhang, Hx; Hu, C. X.; Liu, C. P.; Li, H. F.; Wang, J. S.; Yuan, K. L.; Tang, J. W.; Xu, G. W.
KeywordBioactive Compounds Traditional Chinese Medicines Cell Extract Gas Chromatography-mass Spectrometer Murine Ascites Hepatocarcinoma Cell Elemene Emulsion Injection Zedoary Turmeric Oil And Glucose Injection
Source PublicationJOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
2007-01-04
DOI10.1016/j.jpba.2006.06.033
Volume43Issue:1Pages:151-157
Indexed BySCI
SubtypeArticle
WOS HeadingsScience & Technology ; Physical Sciences ; Life Sciences & Biomedicine
WOS SubjectChemistry, Analytical ; Pharmacology & Pharmacy
WOS Research AreaChemistry ; Pharmacology & Pharmacy
WOS KeywordIMMOBILIZED LIPOSOME CHROMATOGRAPHY ; PARTITION-COEFFICIENTS ; MEMBRANE INTERACTIONS ; ANGELICA-SINENSIS ; ZEDOARIAE-RHIZOMA ; ESSENTIAL OIL ; SESQUITERPENES ; PROTEIN ; DRUGS ; PHASE
AbstractAs the cost of drug development is always many times more than that of drug discovery, predictive methods aiding in the screening of bioavailable drug candidates are of profound significance. In this paper, a novel method for screening bioactive compounds from traditional Chinese medicines (TCMs) was developed by using living cell extract and gas chromatography (GC)-mass spectrometer (MS). The method was validated by using elemene emulsion injection (EEI), a typical TCM with known active compound, to interact with murine ascites hepatocarcinoma cell strain with high metastatic potential (HCa-F). Finally, the method was applied to screen the bioactive compounds from multi-component zedoary turmeric oil and glucose injection (ZTOGI). After HCa-F cells was incubated in ZTOGI, ethyl acetate (EtOAc) was used to extract the compounds in the cells for GC-MS analysis. Fourteen compounds were detected in the desorption eluate of HCa-F cell extract of ZTOGI, and further identified by MS. Curzerene and beta-elemene were found to be two major bioactive compounds in ZTOGI. These results show that the method developed may be applied to quickly screen the potential bioactive components in TCMs interacting with the target cells. (c) 2006 Elsevier B.V. All rights reserved.
Language英语
WOS IDWOS:000243554900018
Citation statistics
Cited Times:49[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://cas-ir.dicp.ac.cn/handle/321008/140564
Collection中国科学院大连化学物理研究所
Affiliation1.Chinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatog R&A Ctr, Dalian 116023, Peoples R China
2.Dalian Med Univ, Dept Pathol, Dalian 116027, Peoples R China
Recommended Citation
GB/T 7714
Zhang, Hx,Hu, C. X.,Liu, C. P.,et al. Screening and analysis of bioactive compounds in traditional Chinese medicines using cell extract and gas chromatography-mass spectrometry[J]. JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS,2007,43(1):151-157.
APA Zhang, Hx.,Hu, C. X..,Liu, C. P..,Li, H. F..,Wang, J. S..,...&Xu, G. W..(2007).Screening and analysis of bioactive compounds in traditional Chinese medicines using cell extract and gas chromatography-mass spectrometry.JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS,43(1),151-157.
MLA Zhang, Hx,et al."Screening and analysis of bioactive compounds in traditional Chinese medicines using cell extract and gas chromatography-mass spectrometry".JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS 43.1(2007):151-157.
Files in This Item:
There are no files associated with this item.
Related Services
Recommend this item
Bookmark
Usage statistics
Export to Endnote
Google Scholar
Similar articles in Google Scholar
[Zhang, Hx]'s Articles
[Hu, C. X.]'s Articles
[Liu, C. P.]'s Articles
Baidu academic
Similar articles in Baidu academic
[Zhang, Hx]'s Articles
[Hu, C. X.]'s Articles
[Liu, C. P.]'s Articles
Bing Scholar
Similar articles in Bing Scholar
[Zhang, Hx]'s Articles
[Hu, C. X.]'s Articles
[Liu, C. P.]'s Articles
Terms of Use
No data!
Social Bookmark/Share
All comments (0)
No comment.
 

Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.