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Screening and analysis of bioactive compounds in traditional Chinese medicines using cell extract and gas chromatography-mass spectrometry
Zhang, Hx; Hu, C. X.; Liu, C. P.; Li, H. F.; Wang, J. S.; Yuan, K. L.; Tang, J. W.; Xu, G. W.
关键词Bioactive Compounds Traditional Chinese Medicines Cell Extract Gas Chromatography-mass Spectrometer Murine Ascites Hepatocarcinoma Cell Elemene Emulsion Injection Zedoary Turmeric Oil And Glucose Injection
刊名JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
2007-01-04
DOI10.1016/j.jpba.2006.06.033
43期:1页:151-157
收录类别SCI
文章类型Article
WOS标题词Science & Technology ; Physical Sciences ; Life Sciences & Biomedicine
类目[WOS]Chemistry, Analytical ; Pharmacology & Pharmacy
研究领域[WOS]Chemistry ; Pharmacology & Pharmacy
关键词[WOS]IMMOBILIZED LIPOSOME CHROMATOGRAPHY ; PARTITION-COEFFICIENTS ; MEMBRANE INTERACTIONS ; ANGELICA-SINENSIS ; ZEDOARIAE-RHIZOMA ; ESSENTIAL OIL ; SESQUITERPENES ; PROTEIN ; DRUGS ; PHASE
英文摘要As the cost of drug development is always many times more than that of drug discovery, predictive methods aiding in the screening of bioavailable drug candidates are of profound significance. In this paper, a novel method for screening bioactive compounds from traditional Chinese medicines (TCMs) was developed by using living cell extract and gas chromatography (GC)-mass spectrometer (MS). The method was validated by using elemene emulsion injection (EEI), a typical TCM with known active compound, to interact with murine ascites hepatocarcinoma cell strain with high metastatic potential (HCa-F). Finally, the method was applied to screen the bioactive compounds from multi-component zedoary turmeric oil and glucose injection (ZTOGI). After HCa-F cells was incubated in ZTOGI, ethyl acetate (EtOAc) was used to extract the compounds in the cells for GC-MS analysis. Fourteen compounds were detected in the desorption eluate of HCa-F cell extract of ZTOGI, and further identified by MS. Curzerene and beta-elemene were found to be two major bioactive compounds in ZTOGI. These results show that the method developed may be applied to quickly screen the potential bioactive components in TCMs interacting with the target cells. (c) 2006 Elsevier B.V. All rights reserved.
语种英语
WOS记录号WOS:000243554900018
引用统计
文献类型期刊论文
条目标识符http://cas-ir.dicp.ac.cn/handle/321008/140564
专题中国科学院大连化学物理研究所
作者单位1.Chinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatog R&A Ctr, Dalian 116023, Peoples R China
2.Dalian Med Univ, Dept Pathol, Dalian 116027, Peoples R China
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GB/T 7714
Zhang, Hx,Hu, C. X.,Liu, C. P.,et al. Screening and analysis of bioactive compounds in traditional Chinese medicines using cell extract and gas chromatography-mass spectrometry[J]. JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS,2007,43(1):151-157.
APA Zhang, Hx.,Hu, C. X..,Liu, C. P..,Li, H. F..,Wang, J. S..,...&Xu, G. W..(2007).Screening and analysis of bioactive compounds in traditional Chinese medicines using cell extract and gas chromatography-mass spectrometry.JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS,43(1),151-157.
MLA Zhang, Hx,et al."Screening and analysis of bioactive compounds in traditional Chinese medicines using cell extract and gas chromatography-mass spectrometry".JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS 43.1(2007):151-157.
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