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题名: Screening and analysis of bioactive compounds in traditional Chinese medicines using cell extract and gas chromatography-mass spectrometry
作者: Zhang, Hx;  Hu, C. X.;  Liu, C. P.;  Li, H. F.;  Wang, J. S.;  Yuan, K. L.;  Tang, J. W.;  Xu, G. W.
关键词: bioactive compounds ;  traditional Chinese medicines ;  cell extract ;  gas chromatography-mass spectrometer ;  murine ascites hepatocarcinoma cell ;  elemene emulsion injection ;  zedoary turmeric oil and glucose injection
刊名: JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
发表日期: 2007-01-04
DOI: 10.1016/j.jpba.2006.06.033
卷: 43, 期:1, 页:151-157
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology ;  Physical Sciences ;  Life Sciences & Biomedicine
类目[WOS]: Chemistry, Analytical ;  Pharmacology & Pharmacy
研究领域[WOS]: Chemistry ;  Pharmacology & Pharmacy
英文摘要: As the cost of drug development is always many times more than that of drug discovery, predictive methods aiding in the screening of bioavailable drug candidates are of profound significance. In this paper, a novel method for screening bioactive compounds from traditional Chinese medicines (TCMs) was developed by using living cell extract and gas chromatography (GC)-mass spectrometer (MS). The method was validated by using elemene emulsion injection (EEI), a typical TCM with known active compound, to interact with murine ascites hepatocarcinoma cell strain with high metastatic potential (HCa-F). Finally, the method was applied to screen the bioactive compounds from multi-component zedoary turmeric oil and glucose injection (ZTOGI). After HCa-F cells was incubated in ZTOGI, ethyl acetate (EtOAc) was used to extract the compounds in the cells for GC-MS analysis. Fourteen compounds were detected in the desorption eluate of HCa-F cell extract of ZTOGI, and further identified by MS. Curzerene and beta-elemene were found to be two major bioactive compounds in ZTOGI. These results show that the method developed may be applied to quickly screen the potential bioactive components in TCMs interacting with the target cells. (c) 2006 Elsevier B.V. All rights reserved.
关键词[WOS]: IMMOBILIZED LIPOSOME CHROMATOGRAPHY ;  PARTITION-COEFFICIENTS ;  MEMBRANE INTERACTIONS ;  ANGELICA-SINENSIS ;  ZEDOARIAE-RHIZOMA ;  ESSENTIAL OIL ;  SESQUITERPENES ;  PROTEIN ;  DRUGS ;  PHASE
语种: 英语
WOS记录号: WOS:000243554900018
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/140564
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Chinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatog R&A Ctr, Dalian 116023, Peoples R China
2.Dalian Med Univ, Dept Pathol, Dalian 116027, Peoples R China

Recommended Citation:
Zhang, Hx,Hu, C. X.,Liu, C. P.,et al. Screening and analysis of bioactive compounds in traditional Chinese medicines using cell extract and gas chromatography-mass spectrometry[J]. JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS,2007,43(1):151-157.
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