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Purification and characterization of a novel ginsenoside-hydrolyzing beta-D-glucosidase from the China white jade snail (Achatina fulica)
Hu, Ying; Luan, Hongwei; Hao, Dacheng; Xiao, Hongbin; Yang, Shengli; Yang, Ling
关键词Beta-d-glucosidase Enzyme Purification Ginsenosides Compound k China White Jade Snail
刊名ENZYME AND MICROBIAL TECHNOLOGY
2007-04-03
DOI10.1016/j.enzmictec.2006.10.011
40期:5页:1358-1366
收录类别SCI
文章类型Article
WOS标题词Science & Technology ; Life Sciences & Biomedicine
类目[WOS]Biotechnology & Applied Microbiology
研究领域[WOS]Biotechnology & Applied Microbiology
关键词[WOS]ANAEROBIC BACTERIUM ; THERMOPHILIC FUNGUS ; SAPONIN METABOLITE ; COMPOUND-K ; IN-VITRO ; STABILITY ; PROTEINS ; CYTOTOXICITY ; SPECIFICITY ; ENHANCEMENT
英文摘要A beta-D-glucosidase, with broad regiospecific activity and designated as G H, was purified to homogeneity from the acetone power of viscera of the China white jade snail (Achatina fulica). G II consisted of two identical subunits (110 kDa) with a native molecular mass of 220 kDa. G II was stable over a wide pH range (4-10 at 30 degrees C for 24 h) and at a relatively high temperature (50 degrees C for 8 h). Moreover, it can cleave both beta-(1 -> 2)-glucosidic linkage at 3-C and beta-(1 -> 6)-glucosidic linkage at 20-C of ginsenosides and can hydrolyze ginsenosides Rb-1, Rb-2, Rb-3 and Rc into their active metabolites, compound K, compound Y, Mx, and Mc, respectively. The optimal activity against p-nitrophenyl-beta-D-glucopyranoside (pNPG) was at 50 degrees C and pH 5.0. Under the same condition, the K-m for pNPG was 0.224 mM with a Y-max of 0.203 mmol nitrophenol min(-1) mg(-1). Of the substrates tested, G II specifically hydrolyzed the beta-D-glucosides involving aryl-beta-glucosides, alkyl-beta-glucosides, and beta-linked disaccharides (i.e. sophorose, gentiobiose, and cellobiose). These findings make the novel enzyme potentially useful in biotransformation processes as well as in the modification of multiple ginsenosides. (c) 2006 Elsevier Inc. All rights reserved.
语种英语
WOS记录号WOS:000245519000053
引用统计
文献类型期刊论文
条目标识符http://cas-ir.dicp.ac.cn/handle/321008/140590
专题中国科学院大连化学物理研究所
作者单位1.Chinese Acad Sci, Lab Pharmaceut Resource Discovery, Dalian Inst Chem Phys, Dalian 116023, Peoples R China
2.Grad Univ Chinese Acad Sci, Beijing, Peoples R China
3.Chinese Acad Sci, Dalian Inst Chem Phys, Med Chem Lab, Dalian, Peoples R China
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Hu, Ying,Luan, Hongwei,Hao, Dacheng,et al. Purification and characterization of a novel ginsenoside-hydrolyzing beta-D-glucosidase from the China white jade snail (Achatina fulica)[J]. ENZYME AND MICROBIAL TECHNOLOGY,2007,40(5):1358-1366.
APA Hu, Ying,Luan, Hongwei,Hao, Dacheng,Xiao, Hongbin,Yang, Shengli,&Yang, Ling.(2007).Purification and characterization of a novel ginsenoside-hydrolyzing beta-D-glucosidase from the China white jade snail (Achatina fulica).ENZYME AND MICROBIAL TECHNOLOGY,40(5),1358-1366.
MLA Hu, Ying,et al."Purification and characterization of a novel ginsenoside-hydrolyzing beta-D-glucosidase from the China white jade snail (Achatina fulica)".ENZYME AND MICROBIAL TECHNOLOGY 40.5(2007):1358-1366.
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