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Purification and characterization of a novel ginsenoside-hydrolyzing beta-D-glucosidase from the China white jade snail (Achatina fulica)
Hu, Ying; Luan, Hongwei; Hao, Dacheng; Xiao, Hongbin; Yang, Shengli; Yang, Ling
KeywordBeta-d-glucosidase Enzyme Purification Ginsenosides Compound k China White Jade Snail
Source PublicationENZYME AND MICROBIAL TECHNOLOGY
2007-04-03
DOI10.1016/j.enzmictec.2006.10.011
Volume40Issue:5Pages:1358-1366
Indexed BySCI
SubtypeArticle
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
WOS SubjectBiotechnology & Applied Microbiology
WOS Research AreaBiotechnology & Applied Microbiology
WOS KeywordANAEROBIC BACTERIUM ; THERMOPHILIC FUNGUS ; SAPONIN METABOLITE ; COMPOUND-K ; IN-VITRO ; STABILITY ; PROTEINS ; CYTOTOXICITY ; SPECIFICITY ; ENHANCEMENT
AbstractA beta-D-glucosidase, with broad regiospecific activity and designated as G H, was purified to homogeneity from the acetone power of viscera of the China white jade snail (Achatina fulica). G II consisted of two identical subunits (110 kDa) with a native molecular mass of 220 kDa. G II was stable over a wide pH range (4-10 at 30 degrees C for 24 h) and at a relatively high temperature (50 degrees C for 8 h). Moreover, it can cleave both beta-(1 -> 2)-glucosidic linkage at 3-C and beta-(1 -> 6)-glucosidic linkage at 20-C of ginsenosides and can hydrolyze ginsenosides Rb-1, Rb-2, Rb-3 and Rc into their active metabolites, compound K, compound Y, Mx, and Mc, respectively. The optimal activity against p-nitrophenyl-beta-D-glucopyranoside (pNPG) was at 50 degrees C and pH 5.0. Under the same condition, the K-m for pNPG was 0.224 mM with a Y-max of 0.203 mmol nitrophenol min(-1) mg(-1). Of the substrates tested, G II specifically hydrolyzed the beta-D-glucosides involving aryl-beta-glucosides, alkyl-beta-glucosides, and beta-linked disaccharides (i.e. sophorose, gentiobiose, and cellobiose). These findings make the novel enzyme potentially useful in biotransformation processes as well as in the modification of multiple ginsenosides. (c) 2006 Elsevier Inc. All rights reserved.
Language英语
WOS IDWOS:000245519000053
Citation statistics
Cited Times:26[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://cas-ir.dicp.ac.cn/handle/321008/140590
Collection中国科学院大连化学物理研究所
Affiliation1.Chinese Acad Sci, Lab Pharmaceut Resource Discovery, Dalian Inst Chem Phys, Dalian 116023, Peoples R China
2.Grad Univ Chinese Acad Sci, Beijing, Peoples R China
3.Chinese Acad Sci, Dalian Inst Chem Phys, Med Chem Lab, Dalian, Peoples R China
Recommended Citation
GB/T 7714
Hu, Ying,Luan, Hongwei,Hao, Dacheng,et al. Purification and characterization of a novel ginsenoside-hydrolyzing beta-D-glucosidase from the China white jade snail (Achatina fulica)[J]. ENZYME AND MICROBIAL TECHNOLOGY,2007,40(5):1358-1366.
APA Hu, Ying,Luan, Hongwei,Hao, Dacheng,Xiao, Hongbin,Yang, Shengli,&Yang, Ling.(2007).Purification and characterization of a novel ginsenoside-hydrolyzing beta-D-glucosidase from the China white jade snail (Achatina fulica).ENZYME AND MICROBIAL TECHNOLOGY,40(5),1358-1366.
MLA Hu, Ying,et al."Purification and characterization of a novel ginsenoside-hydrolyzing beta-D-glucosidase from the China white jade snail (Achatina fulica)".ENZYME AND MICROBIAL TECHNOLOGY 40.5(2007):1358-1366.
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