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题名: Over-expression, purification, and characterization of recombinant NAD-malic enzyme from Escherichia coli K12
作者: Wang, Jinxia;  Tan, Haidong;  Zhao, Zongbao (Kent)
关键词: malic enzyme ;  Escherichia coli K12 ;  over-expression ;  enzyme kinetics
刊名: PROTEIN EXPRESSION AND PURIFICATION
发表日期: 2007-05-01
DOI: 10.1016/j.pep.2006.11.017
卷: 53, 期:1, 页:97-103
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology ;  Life Sciences & Biomedicine
类目[WOS]: Biochemical Research Methods ;  Biochemistry & Molecular Biology ;  Biotechnology & Applied Microbiology
研究领域[WOS]: Biochemistry & Molecular Biology ;  Biotechnology & Applied Microbiology
英文摘要: NAD+-dependent malic enzyme (NAD-ME) gene from Escherichia coli K12 was inserted into an expression vector pET24b(+) and transformed into E coli BL21 (DE3). Recombinant NAD-ME was expressed upon IPTG induction, purified with affinity chromatography, and biochemically characterized. The results showed that recombinant NAD-ME could be produced mainly in a soluble form. The monomeric molecular weight of recombinant NAD-ME was about 65 kDa, whereas monomer, homotetramer, and homooctamer were formed in solution as revealed by nondenaturing polyacrylamide gel electrophoresis analysis. Finally, the K-m, values of NAD-ME for L-malate and NAD were determined as 0.420 +/- 0.174 and 0.097 +/- 0.038 mM, respectively, at pH 7.2. By using this over-expression and purification system, recombinant E coli K12 NAD-ME can now be obtained in large quantity necessary for further biochemical characterization and applications. (c) 2006 Elsevier Inc. All rights reserved.
关键词[WOS]: POLYACRYLAMIDE-GEL ELECTROPHORESIS ;  MOLECULAR-WEIGHTS ;  COLI ;  BINDING ;  CLONING ;  GENE ;  SITE ;  RESIDUES ;  PIGEON
语种: 英语
WOS记录号: WOS:000246245000011
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/140630
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Chinese Acad Sci, Dalian Inst Chem Phys, Dalian 116023, Peoples R China
2.Chinese Acad Sci, Grad Sch, Beijing 100049, Peoples R China

Recommended Citation:
Wang, Jinxia,Tan, Haidong,Zhao, Zongbao . Over-expression, purification, and characterization of recombinant NAD-malic enzyme from Escherichia coli K12[J]. PROTEIN EXPRESSION AND PURIFICATION,2007,53(1):97-103.
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