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题名: Advances in hyphenated analytical techniques for shotgun proteome and peptidome analysis - A review
作者: Hu, Lianghai;  Ye, Mingliang;  Jiang, Xiaogang;  Feng, Shun;  Zou, Hanfa
关键词: proteomics ;  peptidomics ;  hyphenated techniques ;  multidimensional separation ;  liquid chromatography-mass spectrometry ;  review
刊名: ANALYTICA CHIMICA ACTA
发表日期: 2007-08-29
DOI: 10.1016/j.aca.2007.07.046
卷: 598, 期:2, 页:193-204
收录类别: SCI
文章类型: Review
WOS标题词: Science & Technology ;  Physical Sciences
类目[WOS]: Chemistry, Analytical
研究领域[WOS]: Chemistry
英文摘要: Proteomics is defined as the analysis of part or all of the protein components of a complex biological system (a cell, organ or tissue) at a given moment. Due to the huge number of proteins encoded by the genome, novel analytical techniques must be developed to meet the need of large scale analysis. This has led to the hyphenation of multiple techniques to achieve this object. Here current status of the hyphenated analytical techniques of one-dimensional and multidimensional liquid chromatography-mass spectrometry for shotgun proteomic analysis is reviewed, and on-line techniques for automated sample preparation and injection are also covered. In addition, the hyphenated techniques for peptidome analysis are also covered. (c) 2007 Elsevier B.V. All rights reserved.
关键词[WOS]: IONIZATION MASS-SPECTROMETRY ;  PHASE LIQUID-CHROMATOGRAPHY ;  ASSISTED-LASER-DESORPTION/IONIZATION ;  MONOLITHIC CAPILLARY COLUMNS ;  HUMAN PLASMA PROTEOME ;  LC-ESI-MS/MS ;  DYNAMIC-RANGE CHARACTERIZATION ;  METAL AFFINITY-CHROMATOGRAPHY ;  MULTIDIMENSIONAL SEPARATION ;  NANOELECTROSPRAY IONIZATION
语种: 英语
WOS记录号: WOS:000249483400002
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/140632
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Chinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatog R&A Ctr, Grad Sch, Dalian 116023, Peoples R China

Recommended Citation:
Hu, Lianghai,Ye, Mingliang,Jiang, Xiaogang,et al. Advances in hyphenated analytical techniques for shotgun proteome and peptidome analysis - A review[J]. ANALYTICA CHIMICA ACTA,2007,598(2):193-204.
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