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Determination of lysozyme at the nanogram level by a resonance light-scattering technique with functionalized CdTe nanoparticles
Li, Juan; He, Xi-Wen; Wu, Yun-Li; Li, Wen-You; Zhang, Yu-Kui
刊名ANALYTICAL SCIENCES
2007-03-01
23期:3页:331-335
收录类别SCI
文章类型Article
WOS标题词Science & Technology ; Physical Sciences
类目[WOS]Chemistry, Analytical
研究领域[WOS]Chemistry
关键词[WOS]QUANTUM DOTS ; AQUEOUS-SOLUTION ; FLUORESCENCE ; HYBRIDIZATION ; NANOCRYSTALS ; PROTEINS ; DNA
英文摘要Nanoparticles of cadmium telluride coated with mercaptoacetic acid were prepared in the water phase. Further, an assay of lysozyme with a sensitivity at the nanogram level is proposed. At pH 7.28, lysozyme with positive charges can interact with CdTe nanoparticles. The resonance light-scattering (RLS) signals of functionalized nano-CdTe were greatly enhanced by lysozyme in the region of 300 - 600 nm, characterized with peaks located at 367, 470 and 533 nm. A linear relationship could be established between the enhanced RLS intensity and the lysozyme concentration in the range of 0.06 - 4.0 mu g mL(-1). The limit of detection was 9.5 ng mL(-1). The contents of lysozyme were determined with recoveries of 95.6 - 104.8% and RSD of 1.5 - 2.3%, respectively. This method is sensitive, rapid, accurate and simple, and provides a new and reliable means for the quantity determination of lysozyme.
语种英语
WOS记录号WOS:000245347900017
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被引频次:15[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://cas-ir.dicp.ac.cn/handle/321008/140642
专题中国科学院大连化学物理研究所
作者单位1.Nankai Univ, Dept Chem, Tianjin 300071, Peoples R China
2.Chinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatog Res & Anal Ctr, Dalian 116011, Peoples R China
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GB/T 7714
Li, Juan,He, Xi-Wen,Wu, Yun-Li,et al. Determination of lysozyme at the nanogram level by a resonance light-scattering technique with functionalized CdTe nanoparticles[J]. ANALYTICAL SCIENCES,2007,23(3):331-335.
APA Li, Juan,He, Xi-Wen,Wu, Yun-Li,Li, Wen-You,&Zhang, Yu-Kui.(2007).Determination of lysozyme at the nanogram level by a resonance light-scattering technique with functionalized CdTe nanoparticles.ANALYTICAL SCIENCES,23(3),331-335.
MLA Li, Juan,et al."Determination of lysozyme at the nanogram level by a resonance light-scattering technique with functionalized CdTe nanoparticles".ANALYTICAL SCIENCES 23.3(2007):331-335.
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