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题名: Determination of lysozyme at the nanogram level by a resonance light-scattering technique with functionalized CdTe nanoparticles
作者: Li, Juan;  He, Xi-Wen;  Wu, Yun-Li;  Li, Wen-You;  Zhang, Yu-Kui
刊名: ANALYTICAL SCIENCES
发表日期: 2007-03-01
卷: 23, 期:3, 页:331-335
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology ;  Physical Sciences
类目[WOS]: Chemistry, Analytical
研究领域[WOS]: Chemistry
英文摘要: Nanoparticles of cadmium telluride coated with mercaptoacetic acid were prepared in the water phase. Further, an assay of lysozyme with a sensitivity at the nanogram level is proposed. At pH 7.28, lysozyme with positive charges can interact with CdTe nanoparticles. The resonance light-scattering (RLS) signals of functionalized nano-CdTe were greatly enhanced by lysozyme in the region of 300 - 600 nm, characterized with peaks located at 367, 470 and 533 nm. A linear relationship could be established between the enhanced RLS intensity and the lysozyme concentration in the range of 0.06 - 4.0 mu g mL(-1). The limit of detection was 9.5 ng mL(-1). The contents of lysozyme were determined with recoveries of 95.6 - 104.8% and RSD of 1.5 - 2.3%, respectively. This method is sensitive, rapid, accurate and simple, and provides a new and reliable means for the quantity determination of lysozyme.
关键词[WOS]: QUANTUM DOTS ;  AQUEOUS-SOLUTION ;  FLUORESCENCE ;  HYBRIDIZATION ;  NANOCRYSTALS ;  PROTEINS ;  DNA
语种: 英语
WOS记录号: WOS:000245347900017
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/140642
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Nankai Univ, Dept Chem, Tianjin 300071, Peoples R China
2.Chinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatog Res & Anal Ctr, Dalian 116011, Peoples R China

Recommended Citation:
Li, Juan,He, Xi-Wen,Wu, Yun-Li,et al. Determination of lysozyme at the nanogram level by a resonance light-scattering technique with functionalized CdTe nanoparticles[J]. ANALYTICAL SCIENCES,2007,23(3):331-335.
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