DICP OpenIR
Luminescence and Raman spectroscopic studies on the damage of tryptophan, histidine and carnosine by singlet oxygen
Wei, Chunying; Song, Bo; Yuan, Jingli; Feng, Zhaochi; Jia, Guoqing; Li, Can
关键词Singlet Oxygen Carnosine Amino Acids Spectroscopy
刊名JOURNAL OF PHOTOCHEMISTRY AND PHOTOBIOLOGY A-CHEMISTRY
2007-06-10
DOI10.1016/j.jphotochem.2007.01.005
189期:1页:39-45
收录类别SCI
文章类型Article
WOS标题词Science & Technology ; Physical Sciences
类目[WOS]Chemistry, Physical
研究领域[WOS]Chemistry
关键词[WOS]SIDE-CHAIN PEROXIDES ; AMINO-ACIDS ; STRUCTURAL-CHANGES ; PROTEINS ; SPECTRA ; IR ; COMPLEXES ; HISTAMINE ; BINDING ; PROBE
英文摘要In connection with the chemical modification of protein, the photooxidation of histidine (His), tryptophan (Trp) and carnosine by singlet oxygen (O-1(2)) is investigated by a Eu3+ luminescence probe ATTA-Eu3+ and UV Raman spectroscopy under physiological conditions (pH 7.5). The solutions containing both the luminescence probe ATTA-Eu3+(1 x 10(-5) M) and the different concentration of the biological targets His, Trp or carnosine were irradiated by a tungsten lamp in the presence of O-1(2) photosensitizer H(2)TMPyP4 (1 x 10(-5) M), the luminescence intensity of the Eu3+ complex probe decreases linearly with increasing the concentration of the biological target. The reaction rate constants of O-1(2) with His, Trp and carnosine were calculated to be 3.2 x 10(8), 7.7 x 10(7) and 1.3 x 10(8) M-1 s(-1), respectively. The results suggest that the luminescence probe ATTA-Eu3+ can be used for detecting the reactions Of O-1(2) with the biological targets quantitatively under physiological conditions. UV Raman spectroscopy probes the structural changes of these biological targets after reaction with O-1(2), indicating that peroxides are main species for the reaction of Trp although the products decomposed by peroxides are main forms for that of His in a buffer solution. The imidazole ring of carnosine is the target of O-1(2), and the peptide bond is almost intact after reaction with O-1(2). (c) 2007 Elsevier B.V. All rights reserved.
语种英语
WOS记录号WOS:000247453200006
引用统计
被引频次:12[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://cas-ir.dicp.ac.cn/handle/321008/140647
专题中国科学院大连化学物理研究所
作者单位1.Chinese Acad Sci, Dalian Inst Chem Phys, State Key Lab Catalysis, Dalian 116023, Peoples R China
2.Chinese Acad Sci, Dalian Inst Chem Phys, Analyt Chem Lab, Dalian 116023, Peoples R China
推荐引用方式
GB/T 7714
Wei, Chunying,Song, Bo,Yuan, Jingli,et al. Luminescence and Raman spectroscopic studies on the damage of tryptophan, histidine and carnosine by singlet oxygen[J]. JOURNAL OF PHOTOCHEMISTRY AND PHOTOBIOLOGY A-CHEMISTRY,2007,189(1):39-45.
APA Wei, Chunying,Song, Bo,Yuan, Jingli,Feng, Zhaochi,Jia, Guoqing,&Li, Can.(2007).Luminescence and Raman spectroscopic studies on the damage of tryptophan, histidine and carnosine by singlet oxygen.JOURNAL OF PHOTOCHEMISTRY AND PHOTOBIOLOGY A-CHEMISTRY,189(1),39-45.
MLA Wei, Chunying,et al."Luminescence and Raman spectroscopic studies on the damage of tryptophan, histidine and carnosine by singlet oxygen".JOURNAL OF PHOTOCHEMISTRY AND PHOTOBIOLOGY A-CHEMISTRY 189.1(2007):39-45.
条目包含的文件
条目无相关文件。
个性服务
推荐该条目
保存到收藏夹
查看访问统计
导出为Endnote文件
谷歌学术
谷歌学术中相似的文章
[Wei, Chunying]的文章
[Song, Bo]的文章
[Yuan, Jingli]的文章
百度学术
百度学术中相似的文章
[Wei, Chunying]的文章
[Song, Bo]的文章
[Yuan, Jingli]的文章
必应学术
必应学术中相似的文章
[Wei, Chunying]的文章
[Song, Bo]的文章
[Yuan, Jingli]的文章
相关权益政策
暂无数据
收藏/分享
所有评论 (0)
暂无评论
 

除非特别说明,本系统中所有内容都受版权保护,并保留所有权利。