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题名: Luminescence and Raman spectroscopic studies on the damage of tryptophan, histidine and carnosine by singlet oxygen
作者: Wei, Chunying;  Song, Bo;  Yuan, Jingli;  Feng, Zhaochi;  Jia, Guoqing;  Li, Can
关键词: singlet oxygen ;  carnosine ;  amino acids ;  spectroscopy
刊名: JOURNAL OF PHOTOCHEMISTRY AND PHOTOBIOLOGY A-CHEMISTRY
发表日期: 2007-06-10
DOI: 10.1016/j.jphotochem.2007.01.005
卷: 189, 期:1, 页:39-45
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology ;  Physical Sciences
类目[WOS]: Chemistry, Physical
研究领域[WOS]: Chemistry
英文摘要: In connection with the chemical modification of protein, the photooxidation of histidine (His), tryptophan (Trp) and carnosine by singlet oxygen (O-1(2)) is investigated by a Eu3+ luminescence probe ATTA-Eu3+ and UV Raman spectroscopy under physiological conditions (pH 7.5). The solutions containing both the luminescence probe ATTA-Eu3+(1 x 10(-5) M) and the different concentration of the biological targets His, Trp or carnosine were irradiated by a tungsten lamp in the presence of O-1(2) photosensitizer H(2)TMPyP4 (1 x 10(-5) M), the luminescence intensity of the Eu3+ complex probe decreases linearly with increasing the concentration of the biological target. The reaction rate constants of O-1(2) with His, Trp and carnosine were calculated to be 3.2 x 10(8), 7.7 x 10(7) and 1.3 x 10(8) M-1 s(-1), respectively. The results suggest that the luminescence probe ATTA-Eu3+ can be used for detecting the reactions Of O-1(2) with the biological targets quantitatively under physiological conditions. UV Raman spectroscopy probes the structural changes of these biological targets after reaction with O-1(2), indicating that peroxides are main species for the reaction of Trp although the products decomposed by peroxides are main forms for that of His in a buffer solution. The imidazole ring of carnosine is the target of O-1(2), and the peptide bond is almost intact after reaction with O-1(2). (c) 2007 Elsevier B.V. All rights reserved.
关键词[WOS]: SIDE-CHAIN PEROXIDES ;  AMINO-ACIDS ;  STRUCTURAL-CHANGES ;  PROTEINS ;  SPECTRA ;  IR ;  COMPLEXES ;  HISTAMINE ;  BINDING ;  PROBE
语种: 英语
WOS记录号: WOS:000247453200006
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/140647
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Chinese Acad Sci, Dalian Inst Chem Phys, State Key Lab Catalysis, Dalian 116023, Peoples R China
2.Chinese Acad Sci, Dalian Inst Chem Phys, Analyt Chem Lab, Dalian 116023, Peoples R China

Recommended Citation:
Wei, Chunying,Song, Bo,Yuan, Jingli,et al. Luminescence and Raman spectroscopic studies on the damage of tryptophan, histidine and carnosine by singlet oxygen[J]. JOURNAL OF PHOTOCHEMISTRY AND PHOTOBIOLOGY A-CHEMISTRY,2007,189(1):39-45.
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