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题名: Determination of amino acids in cell of alkalophilic microorganisms by high performance liquid chromatography and study of metabolizing pathway
作者: Shi Chun-Yun;  Tian Jing;  Gao Peng;  Xu Guo-Wang
关键词: alkalophilic microorganisms ;  cell ;  amino acids ;  high performance liquid chromatography
刊名: CHINESE JOURNAL OF ANALYTICAL CHEMISTRY
发表日期: 2007-07-01
卷: 35, 期:7, 页:1008-1010
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology ;  Physical Sciences
类目[WOS]: Chemistry, Analytical
研究领域[WOS]: Chemistry
英文摘要: A method for the determination of 17 amino acids in cells of alkalophilic microorganisms by HPLC was established. The Sinochrom ODS BP column was used. Mobile phase was acetonitrile: water = 1: 1 (solution A) and 0. 06 mol/L sodium acetate + 0. 1% N, N-dimethyl formamide + 10% acetonitrile, pH was adjusted to 6. 4 with 5% acetic acid (solution B). The flow rate was 0. 8 mL/min, the temperature of column was 35 degrees C and detected wavelength was set at 360 nm. 17 amino acids were separated commendably in 32 min, the recovery accorded with detecting requirement. The method was used to analyze content of amino acids in cells of alkalophilic microorganisms at different ferment time, the diversification trend of amino acids was obtained, and the interrelated metabolizing pathway was discussed.
语种: 英语
WOS记录号: WOS:000249446200016
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/140648
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Dalian Inst Light Ind, Dalian 116034, Peoples R China
2.Chinese Acad Sci, Natl Chromatog Res & Anal Ctr, Dalian Inst Chem Phys, Dalian 116023, Peoples R China

Recommended Citation:
Shi Chun-Yun,Tian Jing,Gao Peng,et al. Determination of amino acids in cell of alkalophilic microorganisms by high performance liquid chromatography and study of metabolizing pathway[J]. CHINESE JOURNAL OF ANALYTICAL CHEMISTRY,2007,35(7):1008-1010.
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