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题名: High-density cultivation of oleaginous yeast Rhodosporidium toruloides Y4 in fed-batch culture
作者: Li, Yonghong;  Zhao, Zongbao (Kent);  Bai, Fengwu
关键词: high-density cell culture ;  Rhorosporidium toruloides Y4 ;  fed-batch culture ;  microbial lipid
刊名: ENZYME AND MICROBIAL TECHNOLOGY
发表日期: 2007-08-02
DOI: 10.1016/j.enzmictec.2007.02.008
卷: 41, 期:3, 页:312-317
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology ;  Life Sciences & Biomedicine
类目[WOS]: Biotechnology & Applied Microbiology
研究领域[WOS]: Biotechnology & Applied Microbiology
英文摘要: Microbial lipid production by the oleaginous yeast Rhorosporidium toruloides Y4 was studied using glucose as carbon source, in order to realize high-density cell culture. Batch cultures demonstrated that there was little inhibitory effect with a substrate concentration of up to 150 g l(-1). Flask fed-batch cultures were run for 25 days and reached a dry biomass and cellular lipid content of 151.5 g l(-1) and 48.0% (w/w), respectively. Using pilot-scale fed-batch cultures in a 15-1 stirred-tank fermenter for 134 h resulted in dry biomass, lipid content and lipid productivity of 106.5 g l(-1), 67.5% (w/w) and 0.54 g l(-1) h(-1), respectively. The fed-batch culture model used here featured initial nutrient-rich media and a pure carbon source with discontinuous feeding. Gas chromatography analysis revealed that lipids from R. toruloides Y4 contained mainly long-chain fatty acids with 16 and 18 carbon atoms. The four major constituent fatty acids were oleic acid, palmitic acid, stearic acid and linoleic acid. A slight increase in stearic acid production was found during the culture process. Based on these compositional data, microbial lipids from R. toruloides Y4 are a potential alternative oil resource for biodiesel production. (c) 2007 Elsevier Inc. All rights reserved.
关键词[WOS]: YARROWIA-LIPOLYTICA ;  LIPID-ACCUMULATION ;  APIOTRICHUM-CURVATUM ;  CARBON ;  ACID ;  GLUCOSE
语种: 英语
WOS记录号: WOS:000247360900018
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/140689
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Dalian Univ Technol, Dept Biosci & Bioengn, Dalian 116023, Peoples R China
2.Chinese Acad Sci, Dalian Inst Chem Phys, Div Biotechnol, Dalian 116023, Peoples R China

Recommended Citation:
Li, Yonghong,Zhao, Zongbao ,Bai, Fengwu. High-density cultivation of oleaginous yeast Rhodosporidium toruloides Y4 in fed-batch culture[J]. ENZYME AND MICROBIAL TECHNOLOGY,2007,41(3):312-317.
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