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Further improvement of hydrostatic pressure sample injection for microchip electrophoresis
Luo, Yong; Zhang, Qingquan; Qin, Jianhua; Lin, Bingcheng
关键词Hydrostatic Pressure Sample Injection Microchip Electrophoresis Microfluidics
刊名ELECTROPHORESIS
2007-12-01
DOI10.1002/elps.200700330
28期:24页:4769-4771
收录类别SCI
文章类型Article
WOS标题词Science & Technology ; Life Sciences & Biomedicine ; Physical Sciences
类目[WOS]Biochemical Research Methods ; Chemistry, Analytical
研究领域[WOS]Biochemistry & Molecular Biology ; Chemistry
关键词[WOS]CAPILLARY-ELECTROPHORESIS ; MICROFLUIDIC CHIP ; DNA ; SEPARATION ; SYSTEM
英文摘要Hydrostatic pressure sample injection method is able to minimize the number of electrodes needed for a microchip electrophoresis process; however, it neither can be applied for electrophoretic DNA sizing, nor can be implemented on the widely used single-cross microchip. This paper presents an injector design that makes the hydrostatic pressure sample injection method suitable for DNA sizing. By introducing an assistant channel into the normal double-cross injector, a rugged DNA sample plug suitable for sizing can be successfully formed within the cross area during the sample loading. This paper also demonstrates that the hydrostatic pressure sample injection can be performed in the single-cross microchip by controlling the radial position of the detection point in the separation channel. Rhodamine 123 and its derivative as model sample were successfully separated.
语种英语
WOS记录号WOS:000252465600030
引用统计
被引频次:7[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://cas-ir.dicp.ac.cn/handle/321008/140713
专题中国科学院大连化学物理研究所
作者单位Chinese Acad Sci, Dalian Inst Chem Phys, Dalian 116023, Peoples R China
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Luo, Yong,Zhang, Qingquan,Qin, Jianhua,et al. Further improvement of hydrostatic pressure sample injection for microchip electrophoresis[J]. ELECTROPHORESIS,2007,28(24):4769-4771.
APA Luo, Yong,Zhang, Qingquan,Qin, Jianhua,&Lin, Bingcheng.(2007).Further improvement of hydrostatic pressure sample injection for microchip electrophoresis.ELECTROPHORESIS,28(24),4769-4771.
MLA Luo, Yong,et al."Further improvement of hydrostatic pressure sample injection for microchip electrophoresis".ELECTROPHORESIS 28.24(2007):4769-4771.
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