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题名: Further improvement of hydrostatic pressure sample injection for microchip electrophoresis
作者: Luo, Yong1;  Zhang, Qingquan1;  Qin, Jianhua1;  Lin, Bingcheng1
关键词: hydrostatic pressure sample injection ;  microchip electrophoresis ;  microfluidics
刊名: ELECTROPHORESIS
发表日期: 2007-12-01
DOI: 10.1002/elps.200700330
卷: 28, 期:24, 页:4769-4771
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology ;  Life Sciences & Biomedicine ;  Physical Sciences
类目[WOS]: Biochemical Research Methods ;  Chemistry, Analytical
研究领域[WOS]: Biochemistry & Molecular Biology ;  Chemistry
英文摘要: Hydrostatic pressure sample injection method is able to minimize the number of electrodes needed for a microchip electrophoresis process; however, it neither can be applied for electrophoretic DNA sizing, nor can be implemented on the widely used single-cross microchip. This paper presents an injector design that makes the hydrostatic pressure sample injection method suitable for DNA sizing. By introducing an assistant channel into the normal double-cross injector, a rugged DNA sample plug suitable for sizing can be successfully formed within the cross area during the sample loading. This paper also demonstrates that the hydrostatic pressure sample injection can be performed in the single-cross microchip by controlling the radial position of the detection point in the separation channel. Rhodamine 123 and its derivative as model sample were successfully separated.
关键词[WOS]: CAPILLARY-ELECTROPHORESIS ;  MICROFLUIDIC CHIP ;  DNA ;  SEPARATION ;  SYSTEM
语种: 英语
WOS记录号: WOS:000252465600030
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/140713
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Chinese Acad Sci, Dalian Inst Chem Phys, Dalian 116023, Peoples R China

Recommended Citation:
Luo, Yong,Zhang, Qingquan,Qin, Jianhua,et al. Further improvement of hydrostatic pressure sample injection for microchip electrophoresis[J]. ELECTROPHORESIS,2007,28(24):4769-4771.
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