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题名: Fluorescence resonance energy transfer imaging of CFP/YFP labeled NDH in cyanobacterium cell
作者: Ji, Dongmei;  Lv, Wei;  Huang, Zhengxi;  Xia, Andong;  Xu, Min;  Ma, Weimin;  Mi, Hualing;  Ogawa, Teruo
关键词: FRET (fluorescence resonance energy transfer) ;  FLIM (fluorescence lifetime imaging microscopy) ;  CFP/YFP
刊名: JOURNAL OF LUMINESCENCE
发表日期: 2007
DOI: 10.1016/j.jlumin.2006.01.207
卷: 122, 页:463-466
收录类别: ISTP ;  SCI
文章类型: Article
WOS标题词: Science & Technology ;  Physical Sciences
类目[WOS]: Optics
研究领域[WOS]: Optics
英文摘要: The laser confocal scanning microscopy combined with time-correlated single photon counting imaging technique to obtain fluorescence intensity and fluorescence lifetime images for fluorescence resonance energy transfer measurement is reported. Both the fluorescence lifetime imaging microscopy (FLIM) and intensity images show inhomogeneous cyan fluorescent protein and yellow fluorescent protein (CFP/YFP) expression or inhomogeneous energy transfer between CFP and YFP over whole cell. The results presented in this work show that FLIM could be a potential method to reveal the structure-function behavior of NAD(P)H dehydrogenase complexes in living cell. (c) 2006 Elsevier B.V. All rights reserved.
关键词[WOS]: PROTEIN INTERACTIONS ;  MICROSCOPY ;  TRANSPORT ;  NADH
语种: 英语
WOS记录号: WOS:000242991900138
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/140728
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Chinese Acad Sci, Inst Chem, State Key Lab Mol React Dynam, Beijing 100080, Peoples R China
2.Chinese Acad Sci, Shanghai Inst Plant Physiol, Shanghai 200031, Peoples R China

Recommended Citation:
Ji, Dongmei,Lv, Wei,Huang, Zhengxi,et al. Fluorescence resonance energy transfer imaging of CFP/YFP labeled NDH in cyanobacterium cell[J]. JOURNAL OF LUMINESCENCE,2007,122:463-466.
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