DICP OpenIR
Fluorescence resonance energy transfer imaging of CFP/YFP labeled NDH in cyanobacterium cell
Ji, Dongmei; Lv, Wei; Huang, Zhengxi; Xia, Andong; Xu, Min; Ma, Weimin; Mi, Hualing; Ogawa, Teruo
KeywordFret (Fluorescence Resonance Energy Transfer) Flim (Fluorescence Lifetime Imaging Microscopy) Cfp/yfp
Source PublicationJOURNAL OF LUMINESCENCE
2007
DOI10.1016/j.jlumin.2006.01.207
Volume122Pages:463-466
Indexed ByISTP ; SCI
SubtypeArticle
WOS HeadingsScience & Technology ; Physical Sciences
WOS SubjectOptics
WOS Research AreaOptics
WOS KeywordPROTEIN INTERACTIONS ; MICROSCOPY ; TRANSPORT ; NADH
AbstractThe laser confocal scanning microscopy combined with time-correlated single photon counting imaging technique to obtain fluorescence intensity and fluorescence lifetime images for fluorescence resonance energy transfer measurement is reported. Both the fluorescence lifetime imaging microscopy (FLIM) and intensity images show inhomogeneous cyan fluorescent protein and yellow fluorescent protein (CFP/YFP) expression or inhomogeneous energy transfer between CFP and YFP over whole cell. The results presented in this work show that FLIM could be a potential method to reveal the structure-function behavior of NAD(P)H dehydrogenase complexes in living cell. (c) 2006 Elsevier B.V. All rights reserved.
Language英语
WOS IDWOS:000242991900138
Citation statistics
Cited Times:3[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://cas-ir.dicp.ac.cn/handle/321008/140728
Collection中国科学院大连化学物理研究所
Affiliation1.Chinese Acad Sci, Inst Chem, State Key Lab Mol React Dynam, Beijing 100080, Peoples R China
2.Chinese Acad Sci, Shanghai Inst Plant Physiol, Shanghai 200031, Peoples R China
Recommended Citation
GB/T 7714
Ji, Dongmei,Lv, Wei,Huang, Zhengxi,et al. Fluorescence resonance energy transfer imaging of CFP/YFP labeled NDH in cyanobacterium cell[J]. JOURNAL OF LUMINESCENCE,2007,122:463-466.
APA Ji, Dongmei.,Lv, Wei.,Huang, Zhengxi.,Xia, Andong.,Xu, Min.,...&Ogawa, Teruo.(2007).Fluorescence resonance energy transfer imaging of CFP/YFP labeled NDH in cyanobacterium cell.JOURNAL OF LUMINESCENCE,122,463-466.
MLA Ji, Dongmei,et al."Fluorescence resonance energy transfer imaging of CFP/YFP labeled NDH in cyanobacterium cell".JOURNAL OF LUMINESCENCE 122(2007):463-466.
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