DICP OpenIR
Fluorescence resonance energy transfer imaging of CFP/YFP labeled NDH in cyanobacterium cell
Ji, Dongmei; Lv, Wei; Huang, Zhengxi; Xia, Andong; Xu, Min; Ma, Weimin; Mi, Hualing; Ogawa, Teruo
关键词Fret (Fluorescence Resonance Energy Transfer) Flim (Fluorescence Lifetime Imaging Microscopy) Cfp/yfp
刊名JOURNAL OF LUMINESCENCE
2007
DOI10.1016/j.jlumin.2006.01.207
122页:463-466
收录类别ISTP ; SCI
文章类型Article
WOS标题词Science & Technology ; Physical Sciences
类目[WOS]Optics
研究领域[WOS]Optics
关键词[WOS]PROTEIN INTERACTIONS ; MICROSCOPY ; TRANSPORT ; NADH
英文摘要The laser confocal scanning microscopy combined with time-correlated single photon counting imaging technique to obtain fluorescence intensity and fluorescence lifetime images for fluorescence resonance energy transfer measurement is reported. Both the fluorescence lifetime imaging microscopy (FLIM) and intensity images show inhomogeneous cyan fluorescent protein and yellow fluorescent protein (CFP/YFP) expression or inhomogeneous energy transfer between CFP and YFP over whole cell. The results presented in this work show that FLIM could be a potential method to reveal the structure-function behavior of NAD(P)H dehydrogenase complexes in living cell. (c) 2006 Elsevier B.V. All rights reserved.
语种英语
WOS记录号WOS:000242991900138
引用统计
文献类型期刊论文
条目标识符http://cas-ir.dicp.ac.cn/handle/321008/140728
专题中国科学院大连化学物理研究所
作者单位1.Chinese Acad Sci, Inst Chem, State Key Lab Mol React Dynam, Beijing 100080, Peoples R China
2.Chinese Acad Sci, Shanghai Inst Plant Physiol, Shanghai 200031, Peoples R China
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GB/T 7714
Ji, Dongmei,Lv, Wei,Huang, Zhengxi,et al. Fluorescence resonance energy transfer imaging of CFP/YFP labeled NDH in cyanobacterium cell[J]. JOURNAL OF LUMINESCENCE,2007,122:463-466.
APA Ji, Dongmei.,Lv, Wei.,Huang, Zhengxi.,Xia, Andong.,Xu, Min.,...&Ogawa, Teruo.(2007).Fluorescence resonance energy transfer imaging of CFP/YFP labeled NDH in cyanobacterium cell.JOURNAL OF LUMINESCENCE,122,463-466.
MLA Ji, Dongmei,et al."Fluorescence resonance energy transfer imaging of CFP/YFP labeled NDH in cyanobacterium cell".JOURNAL OF LUMINESCENCE 122(2007):463-466.
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