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Multifunctional nanoparticles possessing magnetic, long-lived fluorescence and bio-affinity properties for time-resolved fluorescence cell imaging
Wu, Jing; Ye, Zhiqiang; Wang, Guilan; Yuan, Jingli
关键词Europium Biolabeling Cell Imaging Nanoparticle Time-resolved Fluorescence
刊名TALANTA
2007-07-31
DOI10.1016/j.talanta.2007.03.018
72期:5页:1693-1697
收录类别ISTP ; SCI
文章类型Article
WOS标题词Science & Technology ; Physical Sciences
类目[WOS]Chemistry, Analytical
研究领域[WOS]Chemistry
关键词[WOS]SILICA NANOPARTICLES ; FLUOROMETRIC APPLICATION ; EUROPIUM NANOPARTICLES ; NANOCRYSTALS ; FLUOROIMMUNOASSAY ; PARTICLES ; BACTERIA ; CHELATE
英文摘要Multifunctional nanoparticles possessing magnetic, long-lived fluorescence and bio-affinity properties have been prepared by copolymerization of a conjugate of (3-aminopropyl)triethoxysilane bound to a fluorescent Eu3+ complex, 4,4'-bis(1 '', 1 '', 1 ''-trifluoro-2 '',4 ''-butanedion-4 ''-yl)chlorosulfoo-terphenyl-Eu3+ (APS-BTBCT-Eu3+), free (3-aminopropyl)triethoxysilane (APS) and tetraethyl orthosilicate (TEOS) in the presence of poly(vinylpyrrolidone) (PVP) stabilized magnetic Fe3O4 nanoparticles (similar to 10 nm) with aqueous ammonia in ethanol. The nanoparticles were characterized by transmission electron microscopy (TEM), spectrofluorometry and vibrating sample magnetometry methods. The direct-introduced amino groups on the nanoparticle's surface by using free APS in nanoparticle preparation facilitated the surface modification and bioconjugation of the nanoparticles. The nanoparticle-labeled transferrin was prepared and used for staining the cultured Hela cells. A time-resolved fluorescence imaging technique that can fully eliminate the fast-decaying background noises was developed and used for the fluorescence imaging detection of the cells. A distinct image with the high ratio of signal to noise (S/N) was obtained. (C) 2007 Elsevier B.V. All rights reserved.
语种英语
WOS记录号WOS:000248528100016
引用统计
被引频次:47[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://cas-ir.dicp.ac.cn/handle/321008/140741
专题中国科学院大连化学物理研究所
作者单位1.Chinese Acad Sci, Dalian Inst Chem Phys, Dept Analyt Chem, Dalian 116023, Peoples R China
2.Dalian Univ Technol, Dept Chem, State Key Lab Fine Chem, Dalian 116012, Peoples R China
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Wu, Jing,Ye, Zhiqiang,Wang, Guilan,et al. Multifunctional nanoparticles possessing magnetic, long-lived fluorescence and bio-affinity properties for time-resolved fluorescence cell imaging[J]. TALANTA,2007,72(5):1693-1697.
APA Wu, Jing,Ye, Zhiqiang,Wang, Guilan,&Yuan, Jingli.(2007).Multifunctional nanoparticles possessing magnetic, long-lived fluorescence and bio-affinity properties for time-resolved fluorescence cell imaging.TALANTA,72(5),1693-1697.
MLA Wu, Jing,et al."Multifunctional nanoparticles possessing magnetic, long-lived fluorescence and bio-affinity properties for time-resolved fluorescence cell imaging".TALANTA 72.5(2007):1693-1697.
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