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题名: Multifunctional nanoparticles possessing magnetic, long-lived fluorescence and bio-affinity properties for time-resolved fluorescence cell imaging
作者: Wu, Jing;  Ye, Zhiqiang;  Wang, Guilan;  Yuan, Jingli
关键词: europium ;  biolabeling ;  cell imaging ;  nanoparticle ;  time-resolved fluorescence
刊名: TALANTA
发表日期: 2007-07-31
DOI: 10.1016/j.talanta.2007.03.018
卷: 72, 期:5, 页:1693-1697
收录类别: ISTP ;  SCI
文章类型: Article
WOS标题词: Science & Technology ;  Physical Sciences
类目[WOS]: Chemistry, Analytical
研究领域[WOS]: Chemistry
英文摘要: Multifunctional nanoparticles possessing magnetic, long-lived fluorescence and bio-affinity properties have been prepared by copolymerization of a conjugate of (3-aminopropyl)triethoxysilane bound to a fluorescent Eu3+ complex, 4,4'-bis(1 '', 1 '', 1 ''-trifluoro-2 '',4 ''-butanedion-4 ''-yl)chlorosulfoo-terphenyl-Eu3+ (APS-BTBCT-Eu3+), free (3-aminopropyl)triethoxysilane (APS) and tetraethyl orthosilicate (TEOS) in the presence of poly(vinylpyrrolidone) (PVP) stabilized magnetic Fe3O4 nanoparticles (similar to 10 nm) with aqueous ammonia in ethanol. The nanoparticles were characterized by transmission electron microscopy (TEM), spectrofluorometry and vibrating sample magnetometry methods. The direct-introduced amino groups on the nanoparticle's surface by using free APS in nanoparticle preparation facilitated the surface modification and bioconjugation of the nanoparticles. The nanoparticle-labeled transferrin was prepared and used for staining the cultured Hela cells. A time-resolved fluorescence imaging technique that can fully eliminate the fast-decaying background noises was developed and used for the fluorescence imaging detection of the cells. A distinct image with the high ratio of signal to noise (S/N) was obtained. (C) 2007 Elsevier B.V. All rights reserved.
关键词[WOS]: SILICA NANOPARTICLES ;  FLUOROMETRIC APPLICATION ;  EUROPIUM NANOPARTICLES ;  NANOCRYSTALS ;  FLUOROIMMUNOASSAY ;  PARTICLES ;  BACTERIA ;  CHELATE
语种: 英语
WOS记录号: WOS:000248528100016
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/140741
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Chinese Acad Sci, Dalian Inst Chem Phys, Dept Analyt Chem, Dalian 116023, Peoples R China
2.Dalian Univ Technol, Dept Chem, State Key Lab Fine Chem, Dalian 116012, Peoples R China

Recommended Citation:
Wu, Jing,Ye, Zhiqiang,Wang, Guilan,et al. Multifunctional nanoparticles possessing magnetic, long-lived fluorescence and bio-affinity properties for time-resolved fluorescence cell imaging[J]. TALANTA,2007,72(5):1693-1697.
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