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题名: A lamp light-emitting diode-induced fluorescence detector for capillary electrophoresis
作者: Xu, Jing1;  Xiong, Yan1;  Chen, Shiheng1;  Guan, Yafeng1
关键词: light-emitting diode ;  fluorescence detector ;  filter ;  electrophoresis
刊名: TALANTA
发表日期: 2008-07-15
DOI: 10.1016/j.talanta.2008.03.027
卷: 76, 期:2, 页:369-372
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology ;  Physical Sciences
类目[WOS]: Chemistry, Analytical
研究领域[WOS]: Chemistry
英文摘要: A light-emitting diode-induced fluorescence detector (LED-FD) for capillary electrophoresis was constructed and evaluated. A lamp LED with an enhanced emission spectrum and a band pass filter was used as the excitation light source. Refractive index matching fluid (RIMF) was used in the detection cell to reduce scattering light and the noise level. The limit of detection (LOD) for fluorescein was 1.5 nM (SNR=3). The system exhibited linear responses in the range of 1 x 10(-8) to 5 x 10(-6) M (R=0.999). Application of the lamp LED-FD for the analysis of FITC-labeled ephedra herb extract by capillary electrophoresis was demonstrated. (C) 2008 Elsevier B.V. All rights reserved.
关键词[WOS]: LASER-INDUCED FLUORESCENCE ;  FLOW SYSTEMS ;  PERFORMANCE
语种: 英语
WOS记录号: WOS:000257283100023
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/140752
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Chinese Acad Sci, Dalian Inst Chem Phys, Dept Analyt Chem & Microinstrumentat, Dalian 116023, Peoples R China

Recommended Citation:
Xu, Jing,Xiong, Yan,Chen, Shiheng,et al. A lamp light-emitting diode-induced fluorescence detector for capillary electrophoresis[J]. TALANTA,2008,76(2):369-372.
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