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题名: Identification of the orotidine-5 '-monophosphate decarboxylase gene of the oleaginous yeast Rhodosporidium toruloides
作者: Yang, Fan1, 2;  Zhang, Sufang1;  Tang, Wei1, 2;  Zhao, Zongbao K.1
关键词: Rhodosporidium tortiloides ;  lipids ;  orotidine 5 '-monophosphate decarboxylase ;  restriction-free cloning ;  complementation
刊名: YEAST
发表日期: 2008-09-01
DOI: 10.1002/yea.1607
卷: 25, 期:9, 页:623-630
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology ;  Life Sciences & Biomedicine
类目[WOS]: Biochemistry & Molecular Biology ;  Biotechnology & Applied Microbiology ;  Microbiology ;  Mycology
研究领域[WOS]: Biochemistry & Molecular Biology ;  Biotechnology & Applied Microbiology ;  Microbiology ;  Mycology
英文摘要: Oleaginous yeast Rhodosporidium toraloides is an excellent microbial lipid producer of great industrial potential, yet there is no effective genetic tool for rationally engineering this microorganism. To develop a marker recycling system, the orotidine-5 '-monophosphate (OMP) decarboxylase gene of R. toruloides (RtURA3) was isolated using methods of degenerate pollymerase chain reaction (PCR) together with rapid amplification of cDNA ends. The results showed that RtURA3 contains four extrons and three introns, and that the encoded polypeptide holds a sequence of 279 amino acid residues with significant homology to those of OMP decarboxylases from other yeasts. A shuttle vector pYES2/CT-RtURA3 was constructed via site-specific insertion of RtURA3 into the commercial vector pYES2/CT. Transformation of the shuttle vector into Saccharomyces cerevisiae BY4741, a URA3-deficient yeast strain, ensured the viability of the strain on synthetic dextrose agar plate without uracil, suggesting that the isolated RtURA3 was functionally equivalent to the URA3 gene from S. cerevisiae. The GenBank Accession Nos for the cDNA and genomic DNA sequences of RtURA3 are EU693528 and EU693529, respectively. Copyright (C) 2008 John Wiley & Sons, Ltd.
关键词[WOS]: LIPID-ACCUMULATION ;  TRANSFORMATION ;  SELECTION ;  PLASMIDS ;  CULTURE ;  URA3
语种: 英语
WOS记录号: WOS:000259537700002
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/140760
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Chinese Acad Sci, Dalian Inst Chem Phys, Div Biotechnol, Dalian 116023, Peoples R China
2.Chinese Acad Sci, Grad Sch, Beijing 100049, Peoples R China

Recommended Citation:
Yang, Fan,Zhang, Sufang,Tang, Wei,et al. Identification of the orotidine-5 '-monophosphate decarboxylase gene of the oleaginous yeast Rhodosporidium toruloides[J]. YEAST,2008,25(9):623-630.
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