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Identification of the orotidine-5 '-monophosphate decarboxylase gene of the oleaginous yeast Rhodosporidium toruloides
Yang, Fan1,2; Zhang, Sufang1; Tang, Wei1,2; Zhao, Zongbao K.1
关键词Rhodosporidium Tortiloides Lipids Orotidine 5 '-monophosphate Decarboxylase Restriction-free Cloning Complementation
刊名YEAST
2008-09-01
DOI10.1002/yea.1607
25期:9页:623-630
收录类别SCI
文章类型Article
WOS标题词Science & Technology ; Life Sciences & Biomedicine
类目[WOS]Biochemistry & Molecular Biology ; Biotechnology & Applied Microbiology ; Microbiology ; Mycology
研究领域[WOS]Biochemistry & Molecular Biology ; Biotechnology & Applied Microbiology ; Microbiology ; Mycology
关键词[WOS]LIPID-ACCUMULATION ; TRANSFORMATION ; SELECTION ; PLASMIDS ; CULTURE ; URA3
英文摘要Oleaginous yeast Rhodosporidium toraloides is an excellent microbial lipid producer of great industrial potential, yet there is no effective genetic tool for rationally engineering this microorganism. To develop a marker recycling system, the orotidine-5 '-monophosphate (OMP) decarboxylase gene of R. toruloides (RtURA3) was isolated using methods of degenerate pollymerase chain reaction (PCR) together with rapid amplification of cDNA ends. The results showed that RtURA3 contains four extrons and three introns, and that the encoded polypeptide holds a sequence of 279 amino acid residues with significant homology to those of OMP decarboxylases from other yeasts. A shuttle vector pYES2/CT-RtURA3 was constructed via site-specific insertion of RtURA3 into the commercial vector pYES2/CT. Transformation of the shuttle vector into Saccharomyces cerevisiae BY4741, a URA3-deficient yeast strain, ensured the viability of the strain on synthetic dextrose agar plate without uracil, suggesting that the isolated RtURA3 was functionally equivalent to the URA3 gene from S. cerevisiae. The GenBank Accession Nos for the cDNA and genomic DNA sequences of RtURA3 are EU693528 and EU693529, respectively. Copyright (C) 2008 John Wiley & Sons, Ltd.
语种英语
WOS记录号WOS:000259537700002
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被引频次:5[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://cas-ir.dicp.ac.cn/handle/321008/140760
专题中国科学院大连化学物理研究所
作者单位1.Chinese Acad Sci, Dalian Inst Chem Phys, Div Biotechnol, Dalian 116023, Peoples R China
2.Chinese Acad Sci, Grad Sch, Beijing 100049, Peoples R China
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GB/T 7714
Yang, Fan,Zhang, Sufang,Tang, Wei,et al. Identification of the orotidine-5 '-monophosphate decarboxylase gene of the oleaginous yeast Rhodosporidium toruloides[J]. YEAST,2008,25(9):623-630.
APA Yang, Fan,Zhang, Sufang,Tang, Wei,&Zhao, Zongbao K..(2008).Identification of the orotidine-5 '-monophosphate decarboxylase gene of the oleaginous yeast Rhodosporidium toruloides.YEAST,25(9),623-630.
MLA Yang, Fan,et al."Identification of the orotidine-5 '-monophosphate decarboxylase gene of the oleaginous yeast Rhodosporidium toruloides".YEAST 25.9(2008):623-630.
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