DICP OpenIR
Identification of the orotidine-5 '-monophosphate decarboxylase gene of the oleaginous yeast Rhodosporidium toruloides
Yang, Fan1,2; Zhang, Sufang1; Tang, Wei1,2; Zhao, Zongbao K.1
KeywordRhodosporidium Tortiloides Lipids Orotidine 5 '-monophosphate Decarboxylase Restriction-free Cloning Complementation
Source PublicationYEAST
2008-09-01
DOI10.1002/yea.1607
Volume25Issue:9Pages:623-630
Indexed BySCI
SubtypeArticle
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
WOS SubjectBiochemistry & Molecular Biology ; Biotechnology & Applied Microbiology ; Microbiology ; Mycology
WOS Research AreaBiochemistry & Molecular Biology ; Biotechnology & Applied Microbiology ; Microbiology ; Mycology
WOS KeywordLIPID-ACCUMULATION ; TRANSFORMATION ; SELECTION ; PLASMIDS ; CULTURE ; URA3
AbstractOleaginous yeast Rhodosporidium toraloides is an excellent microbial lipid producer of great industrial potential, yet there is no effective genetic tool for rationally engineering this microorganism. To develop a marker recycling system, the orotidine-5 '-monophosphate (OMP) decarboxylase gene of R. toruloides (RtURA3) was isolated using methods of degenerate pollymerase chain reaction (PCR) together with rapid amplification of cDNA ends. The results showed that RtURA3 contains four extrons and three introns, and that the encoded polypeptide holds a sequence of 279 amino acid residues with significant homology to those of OMP decarboxylases from other yeasts. A shuttle vector pYES2/CT-RtURA3 was constructed via site-specific insertion of RtURA3 into the commercial vector pYES2/CT. Transformation of the shuttle vector into Saccharomyces cerevisiae BY4741, a URA3-deficient yeast strain, ensured the viability of the strain on synthetic dextrose agar plate without uracil, suggesting that the isolated RtURA3 was functionally equivalent to the URA3 gene from S. cerevisiae. The GenBank Accession Nos for the cDNA and genomic DNA sequences of RtURA3 are EU693528 and EU693529, respectively. Copyright (C) 2008 John Wiley & Sons, Ltd.
Language英语
WOS IDWOS:000259537700002
Citation statistics
Cited Times:5[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://cas-ir.dicp.ac.cn/handle/321008/140760
Collection中国科学院大连化学物理研究所
Affiliation1.Chinese Acad Sci, Dalian Inst Chem Phys, Div Biotechnol, Dalian 116023, Peoples R China
2.Chinese Acad Sci, Grad Sch, Beijing 100049, Peoples R China
Recommended Citation
GB/T 7714
Yang, Fan,Zhang, Sufang,Tang, Wei,et al. Identification of the orotidine-5 '-monophosphate decarboxylase gene of the oleaginous yeast Rhodosporidium toruloides[J]. YEAST,2008,25(9):623-630.
APA Yang, Fan,Zhang, Sufang,Tang, Wei,&Zhao, Zongbao K..(2008).Identification of the orotidine-5 '-monophosphate decarboxylase gene of the oleaginous yeast Rhodosporidium toruloides.YEAST,25(9),623-630.
MLA Yang, Fan,et al."Identification of the orotidine-5 '-monophosphate decarboxylase gene of the oleaginous yeast Rhodosporidium toruloides".YEAST 25.9(2008):623-630.
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