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题名: Polyacrylamide-based monolithic capillary column with coating of cellulose tris(3,5-dimethylphenyl-carbamate) for enantiomer separation in capillary electrochromatography
作者: Dong, Xiaoli1, 2;  Wu, Ren'an1;  Dong, Jing1;  Wu, Minghuo1;  Zhu, Yan2;  Zou, Hanfa1
关键词: capillary electrochromatography ;  cellulose tris(3,5-dimethylphenyl-carbamate) ;  enantioseparation ;  polyacrylamide ;  monolithic capillary column
刊名: ELECTROPHORESIS
发表日期: 2008-02-01
DOI: 10.1002/elps.200700644
卷: 29, 期:4, 页:919-927
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology ;  Life Sciences & Biomedicine ;  Physical Sciences
类目[WOS]: Biochemical Research Methods ;  Chemistry, Analytical
研究领域[WOS]: Biochemistry & Molecular Biology ;  Chemistry
英文摘要: A hydrophilic chiral capillary monolithic column for enantiomer separation in CEC was prepared by coating cellulose tris(3,5-dimethylphenyl-carbamate) (CDMPC) on porous hydrophilic poly(acrylamide-co-N,N'-methylene-bisacrylamide) (poly(AA-co-MBA)) monolithic matrix with confine of a fused-silica capillary. The coating conditions were optimized to obtain a stable and reproducible chiral stationary phase for CEC. The effect of organic modifier of ACN in aqueous mobile phase for the enantiomer separation by CEC was investigated, and the significant influence of ACN on the enantioresolution and electrochromatographic retention was observed. Twelve pairs of enantiomers including acidic, neutral, and basic analytes were tested and nine pairs of them were baseline-enantioresolved with acidic and basic aqueous mobile phases. A good within-column repeatability in retention time (RSD = 2.4%) and resolution (RSD = 3.2%) was obtained by consecutive injections of a neutral compound, benzoin, on a prepared chiral monolithic column, while the between-column repeatability in retention time (RSD = 6.4%) and resolution (RSD = 9.6%) was observed by column-to-column examination. The prepared monolithic stationary phase showed good stability in either acidic or basic mobile phase.
关键词[WOS]: CHIRAL STATIONARY-PHASE ;  PERFORMANCE LIQUID-CHROMATOGRAPHY ;  POLYMERIZATION CONDITIONS ;  POROUS PROPERTIES ;  CONTINUOUS BEDS ;  HUMAN SERUM ;  ENANTIOSEPARATIONS ;  GELS ;  TRIS(3,5-DICHLOROPHENYLCARBAMATE) ;  COPOLYMERIZATION
语种: 英语
WOS记录号: WOS:000253575200021
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/140796
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Chinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatog R&A Ctr, Dalian 116023, Peoples R China
2.Zhejiang Univ, Dept Chem, Hangzhou, Peoples R China

Recommended Citation:
Dong, Xiaoli,Wu, Ren'an,Dong, Jing,et al. Polyacrylamide-based monolithic capillary column with coating of cellulose tris(3,5-dimethylphenyl-carbamate) for enantiomer separation in capillary electrochromatography[J]. ELECTROPHORESIS,2008,29(4):919-927.
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