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Capture of phosphopeptides using alpha-zirconium phosphate nanoplatelets
Xu, Songyun1,2; Whitin, John C.1; Yu, Tom To-Sang1; Zhou, Houjiang3; Sun, Dazhi4,5; Sue, Hung-Jue4,5; Zou, Hanfa3; Cohen, Harvey J.1; Zare, Richard N.2
刊名ANALYTICAL CHEMISTRY
2008-07-15
DOI10.1021/ac800577z
80期:14页:5542-5549
收录类别SCI
文章类型Article
WOS标题词Science & Technology ; Physical Sciences
类目[WOS]Chemistry, Analytical
研究领域[WOS]Chemistry
关键词[WOS]ION AFFINITY-CHROMATOGRAPHY ; PROTEIN-PHOSPHORYLATION ANALYSIS ; INORGANIC NANOPOROUS MATERIALS ; MASS-SPECTROMETRY ; PHOSPHOPROTEOME ANALYSIS ; MS ANALYSIS ; IN-VIVO ; ENRICHMENT ; PEPTIDES ; TYROSINE
英文摘要alpha-Zirconium phosphate nanoplatelets (alpha-ZrPN) were studied as a binding agent for phosphopeptides. Nanoplatelets of alpha-zirconium phosphate were incubated overnight with zirconium oxychloride, followed by centrifugation, and washed twice with water followed by an aqueous solution of 80% acetonitrile to form the binding agent. alpha-ZrPN were able specifically to capture phosphoserine-containing peptides from a tryptic digest of a complex peptide mixture in which its abundance was only 0.05%. alpha-ZrPN also bound peptides containing phosphothreonine and phosphotyrosine. The limit of detection for phosphopeptides is similar to 2 fmol, based on using matrix-assisted laser desorption/ionization mass spectrometry. alpha-ZrPN were applied for the analysis of tryptic digests of mouse liver and leukemia cell phosphoproteomes and succeeded in identifying 158 phosphopeptides (209 phosphorylation sites) from 101 phosphoproteins in mouse liver lysate and 78 phosphopeptides (104 phosphorylation sites) from 59 phosphoproteins in leukemia cell extract. For these two tryptic digests, the a-ZrPN approach is able to capture more phosphopeptides than that obtained from TiO2 particles or from Fe3+-IMAC beads, but each method is able to bind some phosphopeptides that the others do not.
语种英语
WOS记录号WOS:000257598100036
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文献类型期刊论文
条目标识符http://cas-ir.dicp.ac.cn/handle/321008/140802
专题中国科学院大连化学物理研究所
作者单位1.Stanford Univ, Sch Med, Dept Pediat, Stanford, CA 94305 USA
2.Stanford Univ, Dept Chem, Stanford, CA 94305 USA
3.Chinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatog R&A Ctr, Dalian 116023, Peoples R China
4.Texas A&M Univ, Dept Mech Engn, College Stn, TX 77843 USA
5.Texas A&M Univ, Polymer Technol Ctr, College Stn, TX 77843 USA
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Xu, Songyun,Whitin, John C.,Yu, Tom To-Sang,et al. Capture of phosphopeptides using alpha-zirconium phosphate nanoplatelets[J]. ANALYTICAL CHEMISTRY,2008,80(14):5542-5549.
APA Xu, Songyun.,Whitin, John C..,Yu, Tom To-Sang.,Zhou, Houjiang.,Sun, Dazhi.,...&Zare, Richard N..(2008).Capture of phosphopeptides using alpha-zirconium phosphate nanoplatelets.ANALYTICAL CHEMISTRY,80(14),5542-5549.
MLA Xu, Songyun,et al."Capture of phosphopeptides using alpha-zirconium phosphate nanoplatelets".ANALYTICAL CHEMISTRY 80.14(2008):5542-5549.
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