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题名: Capture of phosphopeptides using alpha-zirconium phosphate nanoplatelets
作者: Xu, Songyun1, 2;  Whitin, John C.1;  Yu, Tom To-Sang1;  Zhou, Houjiang3;  Sun, Dazhi4, 5;  Sue, Hung-Jue4, 5;  Zou, Hanfa3;  Cohen, Harvey J.1;  Zare, Richard N.2
刊名: ANALYTICAL CHEMISTRY
发表日期: 2008-07-15
DOI: 10.1021/ac800577z
卷: 80, 期:14, 页:5542-5549
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology ;  Physical Sciences
类目[WOS]: Chemistry, Analytical
研究领域[WOS]: Chemistry
英文摘要: alpha-Zirconium phosphate nanoplatelets (alpha-ZrPN) were studied as a binding agent for phosphopeptides. Nanoplatelets of alpha-zirconium phosphate were incubated overnight with zirconium oxychloride, followed by centrifugation, and washed twice with water followed by an aqueous solution of 80% acetonitrile to form the binding agent. alpha-ZrPN were able specifically to capture phosphoserine-containing peptides from a tryptic digest of a complex peptide mixture in which its abundance was only 0.05%. alpha-ZrPN also bound peptides containing phosphothreonine and phosphotyrosine. The limit of detection for phosphopeptides is similar to 2 fmol, based on using matrix-assisted laser desorption/ionization mass spectrometry. alpha-ZrPN were applied for the analysis of tryptic digests of mouse liver and leukemia cell phosphoproteomes and succeeded in identifying 158 phosphopeptides (209 phosphorylation sites) from 101 phosphoproteins in mouse liver lysate and 78 phosphopeptides (104 phosphorylation sites) from 59 phosphoproteins in leukemia cell extract. For these two tryptic digests, the a-ZrPN approach is able to capture more phosphopeptides than that obtained from TiO2 particles or from Fe3+-IMAC beads, but each method is able to bind some phosphopeptides that the others do not.
关键词[WOS]: ION AFFINITY-CHROMATOGRAPHY ;  PROTEIN-PHOSPHORYLATION ANALYSIS ;  INORGANIC NANOPOROUS MATERIALS ;  MASS-SPECTROMETRY ;  PHOSPHOPROTEOME ANALYSIS ;  MS ANALYSIS ;  IN-VIVO ;  ENRICHMENT ;  PEPTIDES ;  TYROSINE
语种: 英语
WOS记录号: WOS:000257598100036
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/140802
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Stanford Univ, Sch Med, Dept Pediat, Stanford, CA 94305 USA
2.Stanford Univ, Dept Chem, Stanford, CA 94305 USA
3.Chinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatog R&A Ctr, Dalian 116023, Peoples R China
4.Texas A&M Univ, Dept Mech Engn, College Stn, TX 77843 USA
5.Texas A&M Univ, Polymer Technol Ctr, College Stn, TX 77843 USA

Recommended Citation:
Xu, Songyun,Whitin, John C.,Yu, Tom To-Sang,et al. Capture of phosphopeptides using alpha-zirconium phosphate nanoplatelets[J]. ANALYTICAL CHEMISTRY,2008,80(14):5542-5549.
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