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题名: Design a chemically defined/medically approved medium for cell transplantation according to the metabolic characteristics of microencapsulated cells and the process of encapsulation
作者: Lv, Guojun1, 2;  Sun, Zhijie1, 2;  Li, Na3;  Li, Shuangyue1, 2;  Zhang, Ying1, 2;  Xie, Yubing1;  Yu, Weiting1, 2;  Wang, Wei1;  Ma, Xiaojun1
关键词: cell transplantation ;  microencapsulation ;  recombinant CHO cell ;  chemically defined medium ;  amino acid
刊名: BIOCHEMICAL ENGINEERING JOURNAL
发表日期: 2008-04-01
DOI: 10.1016/j.bej.2007.08.025
卷: 39, 期:1, 页:157-163
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology ;  Life Sciences & Biomedicine ;  Technology
类目[WOS]: Biotechnology & Applied Microbiology ;  Engineering, Chemical
研究领域[WOS]: Biotechnology & Applied Microbiology ;  Engineering
英文摘要: Cell microencapsulation is a promising therapeutic strategy to many diseases; however, the metabolic characteristics of microencapsulated cells and also the optimum microcapsule additives that meet the criteria for long-term functional immunoisolated transplantation have rarely been taken into consideration hitherto. In this work, the optimized amino acid supplements and several chemically defined compounds were applied to cultivation of the microencapsulated CHO cells. The results show that the optimized medium significantly enhances cell survival and, particularly, endostatin secretion of encapsulated CHO cells over a time period of 8 weeks. Furthermore, long-term in vitro stability of alginate-poly-Llysine-alginate (APA) microcapsules underwent no changes, which indicated that this optimized medium might be feasible to maintain high viability of microencapsulated cell for short periods of time before implantation. (C) 2007 Elsevier B.V. All rights reserved.
关键词[WOS]: MONOCLONAL-ANTIBODY PRODUCTION ;  HUMAN SERUM-ALBUMIN ;  PROTEIN-FREE MEDIUM ;  LYSOPHOSPHATIDIC ACID ;  PHOSPHATIDIC-ACID ;  HYBRIDOMA GROWTH ;  APA MICROCAPSULE ;  CULTURE ;  ALGINATE ;  PROLIFERATION
语种: 英语
WOS记录号: WOS:000254479700019
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/140827
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Chinese Acad Sci, Dalian Inst Chem Phys, Lab Biomed Mat Engn, Dalian 116023, Peoples R China
2.Chinese Acad Sci, Grad Sch, Beijing 100049, Peoples R China
3.Jilin Agr Univ, Grad Sch, Changchun 130118, Peoples R China

Recommended Citation:
Lv, Guojun,Sun, Zhijie,Li, Na,et al. Design a chemically defined/medically approved medium for cell transplantation according to the metabolic characteristics of microencapsulated cells and the process of encapsulation[J]. BIOCHEMICAL ENGINEERING JOURNAL,2008,39(1):157-163.
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