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Design a chemically defined/medically approved medium for cell transplantation according to the metabolic characteristics of microencapsulated cells and the process of encapsulation
Lv, Guojun1,2; Sun, Zhijie1,2; Li, Na3; Li, Shuangyue1,2; Zhang, Ying1,2; Xie, Yubing1; Yu, Weiting1,2; Wang, Wei1; Ma, Xiaojun1
关键词Cell Transplantation Microencapsulation Recombinant Cho Cell Chemically Defined Medium Amino Acid
刊名BIOCHEMICAL ENGINEERING JOURNAL
2008-04-01
DOI10.1016/j.bej.2007.08.025
39期:1页:157-163
收录类别SCI
文章类型Article
WOS标题词Science & Technology ; Life Sciences & Biomedicine ; Technology
类目[WOS]Biotechnology & Applied Microbiology ; Engineering, Chemical
研究领域[WOS]Biotechnology & Applied Microbiology ; Engineering
关键词[WOS]MONOCLONAL-ANTIBODY PRODUCTION ; HUMAN SERUM-ALBUMIN ; PROTEIN-FREE MEDIUM ; LYSOPHOSPHATIDIC ACID ; PHOSPHATIDIC-ACID ; HYBRIDOMA GROWTH ; APA MICROCAPSULE ; CULTURE ; ALGINATE ; PROLIFERATION
英文摘要Cell microencapsulation is a promising therapeutic strategy to many diseases; however, the metabolic characteristics of microencapsulated cells and also the optimum microcapsule additives that meet the criteria for long-term functional immunoisolated transplantation have rarely been taken into consideration hitherto. In this work, the optimized amino acid supplements and several chemically defined compounds were applied to cultivation of the microencapsulated CHO cells. The results show that the optimized medium significantly enhances cell survival and, particularly, endostatin secretion of encapsulated CHO cells over a time period of 8 weeks. Furthermore, long-term in vitro stability of alginate-poly-Llysine-alginate (APA) microcapsules underwent no changes, which indicated that this optimized medium might be feasible to maintain high viability of microencapsulated cell for short periods of time before implantation. (C) 2007 Elsevier B.V. All rights reserved.
语种英语
WOS记录号WOS:000254479700019
引用统计
被引频次:1[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://cas-ir.dicp.ac.cn/handle/321008/140827
专题中国科学院大连化学物理研究所
作者单位1.Chinese Acad Sci, Dalian Inst Chem Phys, Lab Biomed Mat Engn, Dalian 116023, Peoples R China
2.Chinese Acad Sci, Grad Sch, Beijing 100049, Peoples R China
3.Jilin Agr Univ, Grad Sch, Changchun 130118, Peoples R China
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Lv, Guojun,Sun, Zhijie,Li, Na,et al. Design a chemically defined/medically approved medium for cell transplantation according to the metabolic characteristics of microencapsulated cells and the process of encapsulation[J]. BIOCHEMICAL ENGINEERING JOURNAL,2008,39(1):157-163.
APA Lv, Guojun.,Sun, Zhijie.,Li, Na.,Li, Shuangyue.,Zhang, Ying.,...&Ma, Xiaojun.(2008).Design a chemically defined/medically approved medium for cell transplantation according to the metabolic characteristics of microencapsulated cells and the process of encapsulation.BIOCHEMICAL ENGINEERING JOURNAL,39(1),157-163.
MLA Lv, Guojun,et al."Design a chemically defined/medically approved medium for cell transplantation according to the metabolic characteristics of microencapsulated cells and the process of encapsulation".BIOCHEMICAL ENGINEERING JOURNAL 39.1(2008):157-163.
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