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题名: Glass microfluidic chip-based sodium dodecyl sulfate-nongel sieving electrophoresis for determining molecular weights of immunoglobulin fragments
作者: Huang Huai-Qing1;  Dai Zhong-Peng1;  Qin Jian-Hua1;  Lin Bing-Cheng1
关键词: microfluidic chip ;  sodium dodecyl sulfate-nongel sieving electrophoresis ;  protein ;  immunoglobulin ;  molecular weight
刊名: CHINESE JOURNAL OF ANALYTICAL CHEMISTRY
发表日期: 2008-06-01
卷: 36, 期:6, 页:719-723
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology ;  Physical Sciences
类目[WOS]: Chemistry, Analytical
研究领域[WOS]: Chemistry
英文摘要: The concentrations of dextran sieving matrix and running buffer were optimized. Under the optimized conditions (10% (w/v) dextran, 0.1% SDS, 10% glycerol, 0.2 mol/L Tris-boric acid, pH 8.3), six standard proteins could be efficiently separated using sodium dodecyl sulfate (SDS)-nongel sieving electrophoresis on home-made glass microfluidic chip. The relative standard deviations (RSDs) for migration time were lower than 0.50%. A good linear relationship (r =0.994) was built between the migration time of each protein and the logarithm of its molecular weight (logMr). Using the chip-based electrophoretic separation system, the molecular weights of different fragments of immunoglobulin G after different treatments were measured, and the results matched the facts.
关键词[WOS]: CAPILLARY GEL-ELECTROPHORESIS ;  MONOCLONAL-ANTIBODY ;  MICROCHIP
语种: 英语
WOS记录号: WOS:000257877300001
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/140837
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Chinese Acad Sci, Dalian Inst Chem & Phys, Dalian 116023, Peoples R China

Recommended Citation:
Huang Huai-Qing,Dai Zhong-Peng,Qin Jian-Hua,et al. Glass microfluidic chip-based sodium dodecyl sulfate-nongel sieving electrophoresis for determining molecular weights of immunoglobulin fragments[J]. CHINESE JOURNAL OF ANALYTICAL CHEMISTRY,2008,36(6):719-723.
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