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Glass microfluidic chip-based sodium dodecyl sulfate-nongel sieving electrophoresis for determining molecular weights of immunoglobulin fragments
Huang Huai-Qing; Dai Zhong-Peng; Qin Jian-Hua; Lin Bing-Cheng
关键词Microfluidic Chip Sodium Dodecyl Sulfate-nongel Sieving Electrophoresis Protein Immunoglobulin Molecular Weight
刊名CHINESE JOURNAL OF ANALYTICAL CHEMISTRY
2008-06-01
36期:6页:719-723
收录类别SCI
文章类型Article
WOS标题词Science & Technology ; Physical Sciences
类目[WOS]Chemistry, Analytical
研究领域[WOS]Chemistry
关键词[WOS]CAPILLARY GEL-ELECTROPHORESIS ; MONOCLONAL-ANTIBODY ; MICROCHIP
英文摘要The concentrations of dextran sieving matrix and running buffer were optimized. Under the optimized conditions (10% (w/v) dextran, 0.1% SDS, 10% glycerol, 0.2 mol/L Tris-boric acid, pH 8.3), six standard proteins could be efficiently separated using sodium dodecyl sulfate (SDS)-nongel sieving electrophoresis on home-made glass microfluidic chip. The relative standard deviations (RSDs) for migration time were lower than 0.50%. A good linear relationship (r =0.994) was built between the migration time of each protein and the logarithm of its molecular weight (logMr). Using the chip-based electrophoretic separation system, the molecular weights of different fragments of immunoglobulin G after different treatments were measured, and the results matched the facts.
语种英语
WOS记录号WOS:000257877300001
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被引频次:7[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://cas-ir.dicp.ac.cn/handle/321008/140837
专题中国科学院大连化学物理研究所
作者单位Chinese Acad Sci, Dalian Inst Chem & Phys, Dalian 116023, Peoples R China
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GB/T 7714
Huang Huai-Qing,Dai Zhong-Peng,Qin Jian-Hua,et al. Glass microfluidic chip-based sodium dodecyl sulfate-nongel sieving electrophoresis for determining molecular weights of immunoglobulin fragments[J]. CHINESE JOURNAL OF ANALYTICAL CHEMISTRY,2008,36(6):719-723.
APA Huang Huai-Qing,Dai Zhong-Peng,Qin Jian-Hua,&Lin Bing-Cheng.(2008).Glass microfluidic chip-based sodium dodecyl sulfate-nongel sieving electrophoresis for determining molecular weights of immunoglobulin fragments.CHINESE JOURNAL OF ANALYTICAL CHEMISTRY,36(6),719-723.
MLA Huang Huai-Qing,et al."Glass microfluidic chip-based sodium dodecyl sulfate-nongel sieving electrophoresis for determining molecular weights of immunoglobulin fragments".CHINESE JOURNAL OF ANALYTICAL CHEMISTRY 36.6(2008):719-723.
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