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题名: Two-dimensional LC-MS analysis of components in Swertia franchetiana Smith
作者: Liu, Yanming1;  Xu, Qing1;  Xue, Xingya1;  Zhang, Feifang1;  Liang, Xinmiao1
关键词: mass spectrometry ;  on-column focusing ;  Swertia franchetiana Smith ;  traditional Chinese medicine ;  two-dimensional liquid chromatography
刊名: JOURNAL OF SEPARATION SCIENCE
发表日期: 2008-04-01
DOI: 10.1002/jssc.200700393
卷: 31, 期:6-7, 页:935-944
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology ;  Physical Sciences
类目[WOS]: Chemistry, Analytical
研究领域[WOS]: Chemistry
英文摘要: An off-line 2-D-LC approach was developed for the separation and identification of components in extracts of Swertia franchetiana Smith, which is a traditional Chinese medicine. An octadecylsilica column was used for further separation of fractions from a cyano column with UV and MS detection. In this method, a longer column was used in the second dimension to improve the separation ability, and "on-column focusing" was adopted to reduce sample loss and pollution on concentrating the fractions from the first dimension. More than 118 and 575 components were detected in the chloroform and n-butanol extracts by this method, respectively. In contrast, only 20 and 95 peaks were detected by the one-dimensional method. Most of the additionally detected compounds were of low-abundance components. Many compounds can be preliminarily identified according to their UV and mass spectra in the two-dimensional method. The obtained results not only demonstrated the powerful resolution of 2-D-LC, but also led to in-depth comprehension of the UV and mass spectra of complex samples.
关键词[WOS]: PERFORMANCE LIQUID-CHROMATOGRAPHY ;  TRADITIONAL CHINESE MEDICINES ;  MASS-SPECTROMETRY ;  REVERSED-PHASE ;  GAS-CHROMATOGRAPHY ;  AUTOMATED INSTRUMENTATION ;  COLUMN ;  HPLC ;  SEPARATION ;  ONLINE
语种: 英语
WOS记录号: WOS:000255613800005
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/140839
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Chinese Acad Sci, Dalian Inst Chem Phys, Grad Sch, Dalian 116023, Peoples R China

Recommended Citation:
Liu, Yanming,Xu, Qing,Xue, Xingya,et al. Two-dimensional LC-MS analysis of components in Swertia franchetiana Smith[J]. JOURNAL OF SEPARATION SCIENCE,2008,31(6-7):935-944.
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