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题名: Novel two-dimensional reversed-phase liquid chromatography/hydrophilic interaction chromatography, an excellent orthogonal system for practical analysis
作者: Liu, Yanming1;  Xue, Xingya1;  Guo, Zhimou1;  Xu, Qing1;  Zhang, Feifang1;  Liang, Xinmiao1
关键词: Two-dimensional liquid chromatography ;  Cyclodextrin ;  2D-RPLC/HILIC ;  Orthogonality evaluation ;  Low-abundant component ;  Traditional Chinese medicine
刊名: JOURNAL OF CHROMATOGRAPHY A
发表日期: 2008-10-24
DOI: 10.1016/j.chroma.2008.08.079
卷: 1208, 期:1-2, 页:133-140
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology ;  Life Sciences & Biomedicine ;  Physical Sciences
类目[WOS]: Biochemical Research Methods ;  Chemistry, Analytical
研究领域[WOS]: Biochemistry & Molecular Biology ;  Chemistry
英文摘要: A novel two-dimensional reversed-phase liquid chromatography/hydrophilic interaction chromatography (2D-RPLC/HILIC) system is developed with the introduction of the click beta-cyclodextrin (beta-CD) stationary phase. The offline system shows excellent abilities for the separation of polar and medium-polarity components in traditional Chinese medicine (TCM). It facilitates us not only to separate components that cannot be resolved by uni-dimensional chromatography, but also to achieve much more efficient detection of components with low abundance. The orthogonality of this new system is excellent. A simple geometric approach is developed to characterize the practical orthogonality of 2D-LC in the analysis of complex unknown samples. The mathematical characterization results are in good accordance with the experimental findings, and both demonstrate that the proposed 2D-RPLC/HILIC system is a powerful tool for the separation of polar and medium-polarity complex samples. This system may have a great potential for the separation of peptides and metabolomic compounds with similar polarity. (C) 2008 Elsevier B.V. All rights reserved.
关键词[WOS]: HYDROPHILIC-INTERACTION CHROMATOGRAPHY ;  MONOLITHIC SILICA COLUMNS ;  CHIRAL STATIONARY PHASES ;  L. OIL CAKE ;  CARTHAMUS-TINCTORIUS ;  MULTIDIMENSIONAL SEPARATION ;  BETA-CYCLODEXTRIN ;  MASS-SPECTROMETRY ;  POLAR COMPOUNDS ;  PEAK-CAPACITY
语种: 英语
WOS记录号: WOS:000260445900019
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/140860
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Chinese Acad Sci, Dalian Inst Chem Phys, Dalian 116023, Liaoning, Peoples R China

Recommended Citation:
Liu, Yanming,Xue, Xingya,Guo, Zhimou,et al. Novel two-dimensional reversed-phase liquid chromatography/hydrophilic interaction chromatography, an excellent orthogonal system for practical analysis[J]. JOURNAL OF CHROMATOGRAPHY A,2008,1208(1-2):133-140.
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